IP Library › Granted Patent US 12,275,993
Granted Patent B2
US 12,275,993 · App. 18/929,514 · Granted Apr 15, 2025

Analysis of nucleic acid sequences

Inventors: Solongo B. Ziraldo (Pleasanton, CA); Geoffrey McDermott (Livermore, CA); Shea Thompson Lance (Livermore, CA)
Assignee: 10X GENOMICS, INC.
C12Q1/6883C12Q1/6827G16B30/00G16B30/10G16B30/20C12Q2535/122C12Q2600/156
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Quick Facts
Patent No.
US 12,275,993
App. No.
18/929,514
Filed
Oct 28, 2024
Granted
Apr 15, 2025
Kind
B2
Art Unit
1684
USPC
506/4
Abstract

The present disclosure relates to methods, compositions and systems for droplet processing. For example, methods can include (a) providing a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules; (b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and (c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.

Claims (41)

1. A method comprising:

(a) providing:

a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and

a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules;

(b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and

(c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.

2. The method of claim 1 , wherein (b) comprises contacting the first emulsion with the second emulsion to generate a combined emulsion.

3. The method of claim 2 , wherein the lysis reagent is transferred from the first droplet population to the second droplet population via the micelles.

4. The method of claim 3 , wherein the micelles are derived from the first droplet population.

5. The method of claim 1 , wherein the first emulsion comprises a first fluid fraction comprising the micelles, and wherein (b) comprises contacting the second emulsion with the first fluid fraction comprising the micelles.

6. The method of claim 5 , wherein (b) comprises isolating the first fluid fraction comprising the micelles and contacting the second emulsion with the isolated first fluid fraction comprising the micelles.

7. The method of claim 1 , wherein (a) comprises generating the first emulsion or the second emulsion.

8. The method of claim 7 , wherein generating the first emulsion comprises providing a first container comprising i) a first fluid comprising the lysis reagent, and ii) a partitioning fluid that is immiscible with the first fluid; and agitating the first container.

9. The method of claim 8 , wherein the agitating comprises vortexing.

10. The method of claim 7 , wherein generating the second emulsion comprises providing a second container comprising: i) a second fluid comprising a population of cells or nuclei, and a population of particles each coupled to a plurality of nucleic acid barcode molecules, and ii) a partitioning fluid that is immiscible with the second fluid; and agitating the second container.

11. The method of claim 10 , wherein the agitating comprises vortexing.

12. The method of claim 1 , wherein the lysis reagent is a chemical lysis agent.

13. The method of claim 1 , wherein the lysis reagent comprises a surfactant.

14. The method of claim 1 , wherein the lysis reagent comprises n-dodecyl-beta-D-maltoside (DBDM), (N-lauroylsarcosine), sodium dodecyl sulfate (SDS), 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethan-1-ol, or 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate.

15. The method of claim 1 , wherein the lysis reagent comprises a lysis enzyme.

16. The method of claim 1 , wherein the lysis reagent comprises a chemical lysis agent and a lysis enzyme.

17. The method of claim 1 , wherein the first emulsion is a water-in-oil emulsion, the second emulsion is a water-in-oil emulsion, and the micelles are water-in-oil micelles.

18. The method of claim 17 , wherein the micelles are water-in-fluorinated oil micelles.

19. The method of claim 1 , wherein the plurality of nucleic acid barcode molecules is coupled to a particle.

20. The method of claim 1 , wherein the cell or the nucleus comprises a target nucleic acid, and wherein lysing the cell or the nucleus releases the target nucleic acid from the cell or the nucleus.

21. The method of claim 20 , wherein the method further comprises (d) using the target nucleic acid and a nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules to generate a barcoded nucleic acid molecule, wherein the barcoded nucleic acid molecule comprises a sequence of the target nucleic acid or a complement thereof, and a sequence of the nucleic acid barcode molecule or a complement thereof.

22. The method of claim 21 , wherein generating the nucleic acid molecule comprises hybridizing the nucleic acid barcode molecule to the target nucleic acid within the droplet of the second droplet population; and extending the barcoded nucleic acid barcode molecule in a reverse transcription reaction using the target nucleic acid as template.

23. The method of claim 22 , wherein the method further comprises sequencing the barcoded nucleic acid molecule or a derivative thereof.

24. The method of claim 1 , wherein the second droplet population is monodisperse.

25. A method comprising:

(a) generating: 1) a first emulsion comprising a first droplet population and micelles, wherein droplets of the first droplet population and the micelles comprise a chemical lysis agent;

and 2) a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises: i) a cell or a nucleus comprising a target nucleic acid, and ii) a particle coupled to a plurality of nucleic acid barcode molecules;

(b) contacting the first emulsion with the second emulsion, and subjecting the first emulsion and second emulsion to conditions sufficient to transfer the chemical lysis agent to the second droplet population via the micelles comprising the chemical lysis agent; and

(c) lysing the cell or the nucleus within the droplet of the second droplet population with the chemical lysis agent, thereby releasing the target nucleic acid from the cell or the nucleus in the droplet of the second droplet population.

26. The method of claim 25 , wherein the method further comprises, (d) hybridizing the target nucleic acid in the droplet of the second droplet population to a nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules within the droplet of the second droplet population; and (e) using the target nucleic acid hybridized to the nucleic acid barcode molecule to generate a barcoded nucleic acid molecule comprising a sequence of the target nucleic acid or a complement thereof and a sequence of the nucleic acid barcode molecule or a complement thereof.

27. The method of claim 26 , wherein the method comprises sequencing the barcoded nucleic acid molecule or a derivative thereof.

28. The method of claim 25 , wherein:

generating the first emulsion comprises providing a first container comprising i) a first fluid comprising the chemical lysis agent, and ii) a partitioning fluid that is immiscible with the first fluid; and agitating the first container; and

generating the second emulsion comprises providing a second container comprising: i) a second fluid comprising a population of cells or nuclei, and a population of particles each coupled to a plurality of nucleic acid barcode molecules, and ii) a partitioning fluid that is immiscible with the second fluid; and agitating the second container.

29. The method of claim 25 , wherein the chemical lysis agent comprises a surfactant.

30. The method of claim 25 , wherein the chemical lysis agent is n-dodecyl-beta-D-maltoside (DBDM), (N-lauroylsarcosine), sodium dodecyl sulfate (SDS), 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethan-1-ol, or 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 3, 2025
From: ZIRALDO, SOLONGO B.; MCDERMOTT, GEOFFREY; LANCE, SHEA THOMPSON
To: 10X GENOMICS, INC.
Reel/Frame 070383/0992 →
Continuity (5)
Continuation In Part 17397775 · Aug 9, 2021
Continuation PCTUS2020017785 · Feb 11, 2020
Provisional Application 62934256 · Nov 12, 2019
Provisional Application 62804633 · Feb 12, 2019
Related Publication 20250051847A1 · Feb 13, 2025
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