IP Library › Granted Patent US 12,215,366
Granted Patent B2
US 12,215,366 · App. 17/471,935 · Granted Feb 4, 2025

Compositions and methods for epigenome editing

Inventors: Charles A. Gersbach (Chapel Hill, NC); Isaac Hilton (Houston, TX)
Assignee: Duke University
C12N9/22C12N9/1029C12N15/11C12N15/63C12N15/85C12Y203/01048C07K2319/80C12N2310/20
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,215,366
App. No.
17/471,935
Granted
Feb 4, 2025
Kind
B2
Abstract

Disclosed herein are CRISPR/Cas9-based gene activation systems that include a fusion protein of a Cas9 protein and a protein having histone acetyltransferase activity, and methods of using said systems.

Claims (14)

1. A DNA targeting system comprising:

(1) a fusion protein comprising a first polypeptide domain and a second polypeptide domain, wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein comprising the sequence of SEQ ID NO: 1 and the second polypeptide domain comprises a peptide having histone acetyltransferase activity comprising the sequence of SEQ ID NO: 3, wherein the fusion protein activates transcription of a target gene; and

(2) at least one guide RNA (gRNA).

2. The DNA targeting system of claim 1 , wherein the at least one gRNA targets a target region of the target gene.

3. The DNA targeting system of claim 2 , wherein the target region comprises a target enhancer, target regulatory element, a cis-regulatory region of a target gene, or a trans-regulatory region of the target gene.

4. The DNA targeting system of claim 2 , wherein the target region is a distal or proximal cis-regulatory region of the target gene.

5. The DNA targeting system of claim 2 , wherein the target region is located on the same chromosome as the target gene.

6. The DNA targeting system of claim 5 , wherein the target region is located about 1 base pair to about 100,000 base pairs upstream of a transcription start site of the target gene.

7. The DNA targeting system of claim 2 , wherein the target region is located on a different chromosome as the target gene.

8. The DNA targeting system of claim 1 , wherein the target gene is selected from the group consisting of IL1RN, MYOD1, OCT4, HBE, HBG, HBD, HBB, MYOCD, PAX7, FGF1A, FGF1B, and FGF1C.

9. The DNA targeting system of claim 1 , wherein the gRNA comprises a polynucleotide encoded by a sequence selected from SEQ ID NO: 23-27.

10. A method of activating gene expression of a target gene in an isolated cell, the method comprising contacting the isolated cell with one or more polynucleotides encoding the DNA targeting system of claim 1 .

11. The method of claim 10 , wherein the DNA targeting system is delivered to the isolated cell virally or non-virally.

12. The method of claim 10 , wherein the isolated cell is a mammalian cell.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 14, 2021
From: GERSBACH, CHARLES A.; HILTON, ISAAC
To: DUKE UNIVERSITY
Reel/Frame 057473/0817 →
Continuity (4)
Division 16865151 · May 1, 2020
Division 15549842
Provisional Application 62113569 · Feb 9, 2015
Related Publication 20220098561A1 · Mar 31, 2022
References Cited (400)
US 3687808A · Merigan, Jr. · 1972 [cited by applicant]
US 4469863A · Ts'o et al. · 1984 [cited by applicant]
US 4476301A · Imbach et al. · 1984 [cited by applicant]
US 4501729A · Boucher et al. · 1985 [cited by applicant]
US 4554101A · Hopp · 1985 [cited by applicant]
US 4587044A · Miller et al. · 1986 [cited by applicant]
US 4605735A · Miyoshi et al. · 1986 [cited by applicant]
US 4667025A · Miyoshi et al. · 1987 [cited by applicant]
US 4737323A · Martin et al. · 1988 [cited by applicant]
US 4762779A · Snitman · 1988 [cited by applicant]
US 4789737A · Miyoshi et al. · 1988 [cited by applicant]
US 4824941A · Gordon et al. · 1989 [cited by applicant]
US 4828979A · Klevan et al. · 1989 [cited by applicant]
US 4835263A · Nguyen et al. · 1989 [cited by applicant]
US 4845205A · Huynh et al. · 1989 [cited by applicant]
US 4876335A · Yamane et al. · 1989 [cited by applicant]
US 4904582A · Tullis · 1990 [cited by applicant]
US 4948882A · Ruth · 1990 [cited by applicant]
US 4958013A · Letsinger · 1990 [cited by applicant]
US 5013830A · Ohsuka et al. · 1991 [cited by applicant]
US 5023243A · Tullis · 1991 [cited by applicant]
US 5034506A · Summerton et al. · 1991 [cited by applicant]
US 5082830A · Brakel et al. · 1992 [cited by applicant]
US 5109124A · Ramachandran et al. · 1992 [cited by applicant]
US 5112963A · Pieles et al. · 1992 [cited by applicant]
US 5118802A · Smith et al. · 1992 [cited by applicant]
US 5130302A · Spielvogel et al. · 1992 [cited by applicant]
US 5134066A · Rogers et al. · 1992 [cited by applicant]
US 5138045A · Cook et al. · 1992 [cited by applicant]
US 5139941A · Muzyczka et al. · 1992 [cited by applicant]
US 5149797A · Pederson et al. · 1992 [cited by applicant]
US 5166315A · Summerton et al. · 1992 [cited by applicant]
US 5175273A · Bischofberger et al. · 1992 [cited by applicant]
US 5177196A · Meyer et al. · 1993 [cited by applicant]
US 5185444A · Summerton et al. · 1993 [cited by applicant]
US 5188897A · Suhadolnik et al. · 1993 [cited by applicant]
US 5214134A · Weis et al. · 1993 [cited by applicant]
US 5214136A · Lin et al. · 1993 [cited by applicant]
US 5216141A · Benner · 1993 [cited by applicant]
US 5218105A · Cook et al. · 1993 [cited by applicant]
US 5219740A · Miller et al. · 1993 [cited by applicant]
US 5220007A · Pederson et al. · 1993 [cited by applicant]
US 5235033A · Summerton et al. · 1993 [cited by applicant]
US 5245022A · Weis et al. · 1993 [cited by applicant]
US 5254469A · Warren, III · 1993 [cited by applicant]
US 5256775A · Froehler · 1993 [cited by applicant]
US 5258506A · Urdea et al. · 1993 [cited by applicant]
US 5262536A · Hobbs, Jr. · 1993 [cited by applicant]
US 5264423A · Cohen et al. · 1993 [cited by applicant]
US 5264562A · Matteucci · 1993 [cited by applicant]
US 5264564A · Matteucci · 1993 [cited by applicant]
US 5272250A · Spielvogel et al. · 1993 [cited by applicant]
US 5276019A · Cohen et al. · 1994 [cited by applicant]
US 5278302A · Caruthers et al. · 1994 [cited by applicant]
US 5286717A · Cohen et al. · 1994 [cited by applicant]
US 5292873A · Rokita et al. · 1994 [cited by applicant]
US 5317098A · Shizuya et al. · 1994 [cited by applicant]
US 5321131A · Agrawal et al. · 1994 [cited by applicant]
US 5366878A · Pederson et al. · 1994 [cited by applicant]
US 5367066A · Urdea et al. · 1994 [cited by applicant]
US 5371241A · Brush · 1994 [cited by applicant]
US 5391723A · Priest · 1995 [cited by applicant]
US 5399676A · Froehler · 1995 [cited by applicant]
US 5403711A · Walder et al. · 1995 [cited by applicant]
US 5405938A · Summerton et al. · 1995 [cited by applicant]
US 5405939A · Suhadolnik et al. · 1995 [cited by applicant]
US 5414077A · Lin et al. · 1995 [cited by applicant]
US 5416203A · Letsinger · 1995 [cited by applicant]
US 5432272A · Benner · 1995 [cited by applicant]
US 5434257A · Matteucci et al. · 1995 [cited by applicant]
US 5451463A · Nelson et al. · 1995 [cited by applicant]
US 5453496A · Caruthers et al. · 1995 [cited by applicant]
US 5455233A · Spielvogel et al. · 1995 [cited by applicant]
US 5457187A · Gmeiner et al. · 1995 [cited by applicant]
US 5459255A · Cook et al. · 1995 [cited by applicant]
US 5466677A · Baxter et al. · 1995 [cited by applicant]
US 5470967A · Huie et al. · 1995 [cited by applicant]
US 5476925A · Letsinger et al. · 1995 [cited by applicant]
US 5478745A · Samulski et al. · 1995 [cited by applicant]
US 5484908A · Froehler et al. · 1996 [cited by applicant]
US 5486603A · Buhr · 1996 [cited by applicant]
US 5489677A · Sanghvi et al. · 1996 [cited by applicant]
US 5491133A · Walder et al. · 1996 [cited by applicant]
US 5502177A · Matteucci et al. · 1996 [cited by applicant]
US 5510473A · Camerini-otero et al. · 1996 [cited by applicant]
US 5510475A · Agrawal et al. · 1996 [cited by applicant]
US 5512439A · Horner et al. · 1996 [cited by applicant]
US 5512667A · Reed et al. · 1996 [cited by applicant]
US 5514785A · Van Ness et al. · 1996 [cited by applicant]
US 5519126A · Hecht · 1996 [cited by applicant]
US 5525465A · Haralambidis et al. · 1996 [cited by applicant]
US 5525711A · Hawkins et al. · 1996 [cited by applicant]
US 5536821A · Agrawal et al. · 1996 [cited by applicant]
US 5541306A · Agrawal et al. · 1996 [cited by applicant]
US 5541307A · Cook et al. · 1996 [cited by applicant]
US 5541313A · Ruth · 1996 [cited by applicant]
US 5545730A · Urdea et al. · 1996 [cited by applicant]
US 5550111A · Suhadolnik et al. · 1996 [cited by applicant]
US 5552538A · Urdea et al. · 1996 [cited by applicant]
US 5552540A · Haralambidis · 1996 [cited by applicant]
US 5561225A · Maddry et al. · 1996 [cited by applicant]
US 5563253A · Agrawal et al. · 1996 [cited by applicant]
US 5565350A · Kmiec · 1996 [cited by applicant]
US 5565552A · Magda et al. · 1996 [cited by applicant]
US 5567810A · Weis et al. · 1996 [cited by applicant]
US 5571799A · Tkachuk et al. · 1996 [cited by applicant]
US 5574142A · Meyer et al. · 1996 [cited by applicant]
US 5578717A · Urdea et al. · 1996 [cited by applicant]
US 5578718A · Cook et al. · 1996 [cited by applicant]
US 5580731A · Chang et al. · 1996 [cited by applicant]
US 5585481A · Arnold, Jr. et al. · 1996 [cited by applicant]
US 5587361A · Cook et al. · 1996 [cited by applicant]
US 5587371A · Sessler et al. · 1996 [cited by applicant]
US 5587469A · Cook et al. · 1996 [cited by applicant]
US 5591584A · Chang et al. · 1997 [cited by applicant]
US 5593972A · Weiner et al. · 1997 [cited by applicant]
US 5595726A · Magda et al. · 1997 [cited by applicant]
US 5596086A · Matteucci et al. · 1997 [cited by applicant]
US 5596091A · Switzer · 1997 [cited by applicant]
US 5597696A · Linn et al. · 1997 [cited by applicant]
US 5599923A · Sessler et al. · 1997 [cited by applicant]
US 5599928A · Hemmi et al. · 1997 [cited by applicant]
US 5602240A · De Mesmaeker et al. · 1997 [cited by applicant]
US 5608046A · Cook et al. · 1997 [cited by applicant]
US 5610289A · Cook et al. · 1997 [cited by applicant]
US 5614617A · Cook et al. · 1997 [cited by applicant]
US 5618704A · Sanghvi et al. · 1997 [cited by applicant]
US 5623065A · Cook et al. · 1997 [cited by applicant]
US 5623070A · Cook et al. · 1997 [cited by applicant]
US 5625050A · Beaton et al. · 1997 [cited by applicant]
US 5633360A · Bischofberger et al. · 1997 [cited by applicant]
US 5652355A · Metelev et al. · 1997 [cited by applicant]
US 5652356A · Agrawal · 1997 [cited by applicant]
US 5658784A · Eckner et al. · 1997 [cited by applicant]
US 5663312A · Chaturvedula et al. · 1997 [cited by applicant]
US 5677437A · Teng et al. · 1997 [cited by applicant]
US 5677439A · Weis et al. · 1997 [cited by applicant]
US 5681941A · Cook et al. · 1997 [cited by applicant]
US 5688941A · Cook et al. · 1997 [cited by applicant]
US 5700922A · Cook et al. · 1997 [cited by applicant]
US 5714331A · Buchardt et al. · 1998 [cited by applicant]
US 5719262A · Buchardt et al. · 1998 [cited by applicant]
US 5741683A · Zhou et al. · 1998 [cited by applicant]
US 5750692A · Cook et al. · 1998 [cited by applicant]
US 5773700A · Van Grinsven et al. · 1998 [cited by applicant]
US 5962428A · Carrano et al. · 1999 [cited by applicant]
US 6057152A · Samulski et al. · 2000 [cited by applicant]
US 6140081A · Barbas · 2000 [cited by applicant]
US 6204059B1 · Samulski et al. · 2001 [cited by applicant]
US 6207453B1 · Maass et al. · 2001 [cited by applicant]
US 6268213B1 · Samulski et al. · 2001 [cited by applicant]
US 6268490B1 · Imanishi et al. · 2001 [cited by applicant]
US 6287860B1 · Monia et al. · 2001 [cited by applicant]
US 6453242B1 · Eisenberg et al. · 2002 [cited by applicant]
US 6462254B1 · Vernachio et al. · 2002 [cited by applicant]
US 6491907B1 · Rabinowitz et al. · 2002 [cited by applicant]
US 6534261B1 · Cox, III et al. · 2003 [cited by applicant]
US 6596535B1 · Carter · 2003 [cited by applicant]
US 6660514B1 · Zolotukhin et al. · 2003 [cited by applicant]
US 6734291B2 · Kochkine et al. · 2004 [cited by applicant]
US 6770748B2 · Imanishi et al. · 2004 [cited by applicant]
US 6794499B2 · Wengel et al. · 2004 [cited by applicant]
US 6951753B2 · Shenk et al. · 2005 [cited by applicant]
US 7034133B2 · Wengel et al. · 2006 [cited by applicant]
US 7053207B2 · Wengel · 2006 [cited by applicant]
US 7060809B2 · Wengel et al. · 2006 [cited by applicant]
US 7074596B2 · Darzynkiewicz et al. · 2006 [cited by applicant]
US 7084125B2 · Wengel · 2006 [cited by applicant]
US 7094604B2 · Snyder et al. · 2006 [cited by applicant]
US 7125717B2 · Carter · 2006 [cited by applicant]
US 7172893B2 · Rabinowitz et al. · 2007 [cited by applicant]
US 7201898B2 · Monahan et al. · 2007 [cited by applicant]
US 7229823B2 · Samulski et al. · 2007 [cited by applicant]
US 7282199B2 · Gao et al. · 2007 [cited by applicant]
US 7439065B2 · Ferrari et al. · 2008 [cited by applicant]
US 7449561B1 · Sommer et al. · 2008 [cited by applicant]
US 7456683B2 · Takano et al. · 2008 [cited by applicant]
US 7572582B2 · Wengel et al. · 2009 [cited by applicant]
US 7588772B2 · Kay et al. · 2009 [cited by applicant]
US 7728118B2 · Wood et al. · 2010 [cited by applicant]
US 7745651B2 · Heyes et al. · 2010 [cited by applicant]
US 7790449B2 · Gao et al. · 2010 [cited by applicant]
US 7799565B2 · Maclachlan et al. · 2010 [cited by applicant]
US 7906111B2 · Wilson et al. · 2011 [cited by applicant]
US 8278036B2 · Kariko et al. · 2012 [cited by applicant]
US 8450107B1 · Zhang et al. · 2013 [cited by applicant]
US 8586526B2 · Gregory et al. · 2013 [cited by applicant]
US 8697359B1 · Zhang et al. · 2014 [cited by applicant]
US 8889356B2 · Zhang · 2014 [cited by applicant]
US 8993233B2 · Zhang et al. · 2015 [cited by applicant]
US 9139554B2 · Hope et al. · 2015 [cited by applicant]
US 9458205B2 · Gregory et al. · 2016 [cited by applicant]
US 9738879B2 · Gersbach et al. · 2017 [cited by applicant]
US 9828582B2 · Perez-Pinera et al. · 2017 [cited by applicant]
US 9834791B2 · Zhang et al. · 2017 [cited by applicant]
US 9890364B2 · Joung et al. · 2018 [cited by applicant]
US 10011850B2 · Joung et al. · 2018 [cited by applicant]
US 10190106B2 · Wolfe et al. · 2019 [cited by applicant]
US 10266850B2 · Doudna et al. · 2019 [cited by applicant]
US 10676726B2 · Gersbach et al. · 2020 [cited by applicant]
US 10676735B2 · Gersbach et al. · 2020 [cited by applicant]
US 10704060B2 · Gersbach et al. · 2020 [cited by applicant]
US 10711256B2 · Gersbach et al. · 2020 [cited by applicant]
US 10745714B2 · Gersbach et al. · 2020 [cited by applicant]
US 11155796B2 · Gersbach et al. · 2021 [cited by applicant]
US 11421251B2 · Gersbach et al. · 2022 [cited by applicant]
US 11427817B2 · Josephs et al. · 2022 [cited by applicant]
US 20020160940A1 · Case et al. · 2002 [cited by applicant]
US 20040142025A1 · Maclachlan et al. · 2004 [cited by applicant]
US 20040175727A1 · Draghia-Akli et al. · 2004 [cited by applicant]
US 20040192593A1 · Draghia-Akli et al. · 2004 [cited by applicant]
US 20040204345A1 · Case et al. · 2004 [cited by applicant]
US 20060068395A1 · Wood et al. · 2006 [cited by applicant]
US 20060211647A1 · Khan · 2006 [cited by applicant]
US 20070042031A1 · Maclachlan et al. · 2007 [cited by applicant]
US 20070042462A1 · Hildinger · 2007 [cited by applicant]
US 20070059795A1 · Moore et al. · 2007 [cited by applicant]
US 20070185042A1 · Tsai et al. · 2007 [cited by applicant]
US 20070192880A1 · Muyan et al. · 2007 [cited by applicant]
US 20080070299A1 · Wood et al. · 2008 [cited by applicant]
US 20080090291A1 · Wood et al. · 2008 [cited by applicant]
US 20080200409A1 · Wilson et al. · 2008 [cited by applicant]
US 20090018031A1 · Trinklein et al. · 2009 [cited by applicant]
US 20100035968A1 · Rasmussen et al. · 2010 [cited by applicant]
US 20100076057A1 · Sontheimer et al. · 2010 [cited by applicant]
US 20100261175A1 · Rasmussen et al. · 2010 [cited by applicant]
US 20100267018A1 · Wengel et al. · 2010 [cited by applicant]
US 20110145940A1 · Voytas et al. · 2011 [cited by applicant]
US 20110197290A1 · Fahrenkrug et al. · 2011 [cited by applicant]
US 20110236353A1 · Wilson et al. · 2011 [cited by applicant]
US 20110263682A1 · De Kimpe et al. · 2011 [cited by applicant]
US 20110286957A1 · Prieve et al. · 2011 [cited by applicant]
US 20110301073A1 · Gregory et al. · 2011 [cited by applicant]
US 20110301218A1 · Bozzoni et al. · 2011 [cited by applicant]
US 20120195917A1 · Sahin et al. · 2012 [cited by applicant]
US 20120207744A1 · Mendlein et al. · 2012 [cited by applicant]
US 20130274129A1 · Katzen et al. · 2013 [cited by applicant]
US 20130323001A1 · Ueki et al. · 2013 [cited by applicant]
US 20140068797A1 · Doudna et al. · 2014 [cited by applicant]
US 20140140969A1 · Beausejour et al. · 2014 [cited by applicant]
US 20140170753A1 · Zhang · 2014 [cited by applicant]
US 20140179006A1 · Zhang · 2014 [cited by applicant]
US 20140186958A1 · Zhang et al. · 2014 [cited by applicant]
US 20140234975A1 · Silva et al. · 2014 [cited by applicant]
US 20140295557A1 · Joung et al. · 2014 [cited by applicant]
US 20140309177A1 · Perez-Pinera et al. · 2014 [cited by applicant]
US 20140315862A1 · Kaye · 2014 [cited by applicant]
US 20140356956A1 · Church et al. · 2014 [cited by applicant]
US 20140357530A1 · Zhang et al. · 2014 [cited by applicant]
US 20140377868A1 · Joung et al. · 2014 [cited by applicant]
US 20150024499A1 · Brouns et al. · 2015 [cited by applicant]
US 20150031089A1 · Lindstrom · 2015 [cited by applicant]
US 20150044772A1 · Zhao · 2015 [cited by applicant]
US 20150045413A1 · De Visser et al. · 2015 [cited by applicant]
US 20150056705A1 · Conway et al. · 2015 [cited by applicant]
US 20150079064A1 · Gersbach et al. · 2015 [cited by applicant]
US 20150159178A1 · Green et al. · 2015 [cited by applicant]
US 20150166980A1 · Liu et al. · 2015 [cited by applicant]
US 20150225717A1 · Lee et al. · 2015 [cited by applicant]
US 20150252358A1 · Maeder et al. · 2015 [cited by applicant]
US 20160002634A1 · Sazani et al. · 2016 [cited by applicant]
US 20160040189A1 · Kennedy et al. · 2016 [cited by applicant]
US 20160058889A1 · Olson et al. · 2016 [cited by applicant]
US 20160177278A1 · Wolfe et al. · 2016 [cited by applicant]
US 20160199419A1 · Miura · 2016 [cited by applicant]
US 20160201089A1 · Gersbach et al. · 2016 [cited by applicant]
US 20160281166A1 · Bhattacharjee et al. · 2016 [cited by applicant]
US 20160354487A1 · Zhang et al. · 2016 [cited by applicant]
US 20170002316A1 · Gascón Jiménez et al. · 2017 [cited by applicant]
US 20170198308A1 · Qi et al. · 2017 [cited by applicant]
US 20170204407A1 · Gilbert et al. · 2017 [cited by applicant]
US 20170283831A1 · Zhang et al. · 2017 [cited by applicant]
US 20170298331A1 · Gersbach et al. · 2017 [cited by applicant]
US 20170327806A1 · Joung et al. · 2017 [cited by applicant]
US 20170362635A1 · Chamberlain et al. · 2017 [cited by applicant]
US 20180023064A1 · Gersbach et al. · 2018 [cited by applicant]
US 20180073012A1 · Liu et al. · 2018 [cited by applicant]
US 20180094238A1 · Perez-Pinera et al. · 2018 [cited by applicant]
US 20180127780A1 · Liu et al. · 2018 [cited by applicant]
US 20180135109A1 · Jayaram et al. · 2018 [cited by applicant]
US 20180201951A1 · Guilak et al. · 2018 [cited by applicant]
US 20180237771A1 · Kim et al. · 2018 [cited by applicant]
US 20180251735A1 · Ko · 2018 [cited by applicant]
US 20180271069A1 · Min et al. · 2018 [cited by applicant]
US 20180280539A1 · Debs et al. · 2018 [cited by applicant]
US 20180291370A1 · Gersbach et al. · 2018 [cited by applicant]
US 20180305689A1 · Sætrom et al. · 2018 [cited by applicant]
US 20180305704A1 · Zhang · 2018 [cited by applicant]
US 20180305719A1 · Perez-Pinera et al. · 2018 [cited by applicant]
US 20180319850A1 · Payne et al. · 2018 [cited by applicant]
US 20180320197A1 · Gersbach et al. · 2018 [cited by applicant]
US 20180327740A1 · Gifford et al. · 2018 [cited by applicant]
US 20180334685A1 · Yeo et al. · 2018 [cited by applicant]
US 20180334688A1 · Gersbach et al. · 2018 [cited by applicant]
US 20180353615A1 · Gersbach et al. · 2018 [cited by applicant]
US 20190032049A1 · Naldini et al. · 2019 [cited by applicant]
US 20190038776A1 · Pyle et al. · 2019 [cited by applicant]
US 20190048337A1 · Hsu et al. · 2019 [cited by applicant]
US 20190062790A1 · Doudna et al. · 2019 [cited by applicant]
US 20190078119A1 · Wilson et al. · 2019 [cited by applicant]
US 20190106710A1 · Zhang et al. · 2019 [cited by applicant]
US 20190127713A1 · Gersbach et al. · 2019 [cited by applicant]
US 20190134221A1 · Bumcrot et al. · 2019 [cited by applicant]
US 20190136229A1 · Josephs et al. · 2019 [cited by applicant]
US 20190151476A1 · Gersbach et al. · 2019 [cited by applicant]
US 20190183932A1 · Mackall et al. · 2019 [cited by applicant]
US 20190194633A1 · Gersbach et al. · 2019 [cited by applicant]
US 20190201402A1 · Jiang et al. · 2019 [cited by applicant]
US 20190248854A1 · Tremblay et al. · 2019 [cited by applicant]
US 20190264232A1 · Hou et al. · 2019 [cited by applicant]
US 20190351074A1 · Ahituv et al. · 2019 [cited by applicant]
US 20190359959A1 · Jaenisch et al. · 2019 [cited by applicant]
US 20190374655A1 · Kabadi et al. · 2019 [cited by applicant]
US 20200002731A1 · Frendewey et al. · 2020 [cited by applicant]
US 20200056206A1 · Tremblay et al. · 2020 [cited by applicant]
US 20200080108A1 · Jaskula-Ranga et al. · 2020 [cited by applicant]
US 20200109406A1 · Miller et al. · 2020 [cited by applicant]
US 20200123533A1 · Wang et al. · 2020 [cited by applicant]
US 20200216549A1 · Fukumura et al. · 2020 [cited by applicant]
US 20200216810A1 · Metelitsa et al. · 2020 [cited by applicant]
US 20200260698A1 · Kyrychenko et al. · 2020 [cited by applicant]
US 20200275641A1 · Min et al. · 2020 [cited by applicant]
US 20200318139A1 · Gersbach et al. · 2020 [cited by applicant]
US 20200332307A1 · Hummel et al. · 2020 [cited by applicant]
US 20200347105A1 · Gersbach et al. · 2020 [cited by applicant]
US 20200385695A1 · Gersbach et al. · 2020 [cited by applicant]
US 20210002665A1 · Gersbach et al. · 2021 [cited by applicant]
US 20210032654A1 · Gersbach et al. · 2021 [cited by applicant]
US 20210040460A1 · Gersbach et al. · 2021 [cited by applicant]
US 20210322577A1 · Lande et al. · 2021 [cited by applicant]
US 20220177879A1 · Gersbach et al. · 2022 [cited by applicant]
US 20220184229A1 · Gersbach et al. · 2022 [cited by applicant]
US 20220195406A1 · Gersbach et al. · 2022 [cited by applicant]
US 20220305141A1 · Gersbach et al. · 2022 [cited by applicant]
US 20220307015A1 · Gersbach et al. · 2022 [cited by applicant]
US 20220364124A1 · Gersbach et al. · 2022 [cited by applicant]
US 20220396790A1 · Gersbach et al. · 2022 [cited by applicant]
US 20230032846A1 · Gersbach et al. · 2023 [cited by applicant]
US 20230047669A1 · Josephs et al. · 2023 [cited by applicant]
US 20240052328A1 · Kwon et al. · 2024 [cited by applicant]
US 20240058425A1 · Gersbach et al. · 2024 [cited by applicant]
US 20240067968A1 · Cosgrove et al. · 2024 [cited by applicant]
AU 2022318664A1 · 2024 [cited by applicant]
CA 2749305A1 · 2010 [cited by applicant]
CA 2981508A1 · 2016 [cited by applicant]
CA 3086885A1 · 2019 [cited by applicant]
CA 3101477A1 · 2019 [cited by applicant]
EP 2620161A1 · 2013 [cited by applicant]
EP 3009511A2 · 2016 [cited by applicant]
EP 3199632A1 · 2017 [cited by applicant]
EP 3712272A1 · 2020 [cited by applicant]
EP 3209783B1 · 2021 [cited by applicant]
EP 3995584A1 · 2022 [cited by applicant]
JP 2013509159A · 2013 [cited by applicant]
JP 2015534817A · 2015 [cited by applicant]
JP 2016521452A · 2016 [cited by applicant]
JP 2016521452A2 · 2016 [cited by applicant]
JP 2016521975A · 2016 [cited by applicant]
JP 2016523082A · 2016 [cited by applicant]
KR 20190134673A · 2019 [cited by applicant]
WO WO199118114A1 · 1991 [cited by applicant]
WO WO1992000387A1 · 1992 [cited by applicant]
WO WO1993007883A1 · 1993 [cited by applicant]
WO WO1993024640A2 · 1993 [cited by applicant]
WO WO1994016737A1 · 1994 [cited by applicant]
WO WO1998053058A1 · 1998 [cited by applicant]
WO WO1998053059A1 · 1998 [cited by applicant]
WO WO1998053060A1 · 1998 [cited by applicant]
WO WO2000028004A1 · 2000 [cited by applicant]
WO 2001083793A2 · 2001 [cited by applicant]
WO WO2001083783A2 · 2001 [cited by applicant]
WO WO2001092551A2 · 2001 [cited by applicant]
WO WO2002016536A1 · 2002 [cited by applicant]
WO WO2003016496A2 · 2003 [cited by applicant]
WO WO2003042397A2 · 2003 [cited by applicant]
WO 2003072788A1 · 2003 [cited by applicant]
WO WO2005033321A2 · 2005 [cited by applicant]
WO WO2006110689A2 · 2006 [cited by applicant]
WO WO2007019301A2 · 2007 [cited by applicant]
WO WO2008006028A2 · 2008 [cited by applicant]
WO WO2008070859A2 · 2008 [cited by applicant]
WO WO2010053572A2 · 2010 [cited by applicant]
WO WO2010075424A2 · 2010 [cited by applicant]
WO 2010144740A1 · 2010 [cited by applicant]
WO WO2011036640A2 · 2011 [cited by applicant]
WO WO2011126808A2 · 2011 [cited by applicant]
WO WO2011141820A1 · 2011 [cited by applicant]
WO WO2011154427A1 · 2011 [cited by applicant]
WO WO2012136476A1 · 2012 [cited by applicant]
WO WO2012170930A1 · 2012 [cited by applicant]
WO WO2013049493A1 · 2013 [cited by applicant]
WO WO2013098244A1 · 2013 [cited by applicant]
WO WO2013143555A1 · 2013 [cited by applicant]
WO WO2013163628A2 · 2013 [cited by applicant]
WO WO2013176772A1 · 2013 [cited by applicant]
WO WO2013182683A1 · 2013 [cited by applicant]
WO WO2014018423A2 · 2014 [cited by applicant]
WO WO2014059255A1 · 2014 [cited by applicant]
WO WO2014065596A1 · 2014 [cited by applicant]
WO WO2014081855A1 · 2014 [cited by applicant]
Cited By (2)
US 12,428,631 US 12,629,428