Methods for carbon capture and increasing yield of plants
A method of increasing organic carbon in a soil is disclosed. The method includes inoculating the soil and/or a plant growing in the soil with one or more fungal strains from at least one genus selected from the group consisting of Acrocalymma, Clonostachys, Leptodontidium, Periconia, Phaeosphaeria, Thozetella, Trichoderma , and a combination thereof, wherein the one or more fungal strains are in an amount effective to increase organic carbon in the soil compared to a non-inoculated control soil. Also disclosed is a method of enhancing plant growth, comprising: applying to a plant, a plant part, or the locus surrounding the plant with one or more fungal strains from at least one genus selected from the group consisting of Acrocalymma, Clonostachys, Leptodontidium, Periconia, Phaeosphaeria, Thozetella, Trichoderma , and a combination thereof in an amount effective to enhance the growth of the plant as compared to an untreated control plant.
1. A method of increasing organic carbon in a soil, comprising:
inoculating the soil and/or a plant growing in the soil with one or more fungal strains having a nuclear ribosomal internal transcribed spacer 2 (ITS2) sequence that is at least 90% identical to the nucleotide sequence of SEQ ID No: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11. 12, 13, 14, 15, 16, 17, 18, or 19;
wherein the one or more fungal strains are selected from the group consisting of Acrocalymma vagum DMTR-CTR-11556 (NMI Accession No. V22/006357), Leptodontidium orchidicola DMTR-CTR-4873 (NMI Accession No. V22/003497), Periconia sp. DMTR-CTR-6649 (NMI Accession No. V22/006356), Periconia macrospinosa DMTR-CTR-US-125 (ATCC Accession No. PTA-127300), Periconia macrospinosa DMTR-CTR-1852 (NMI Accession No. V22/006358), Phaeosphaeria luctuosa/vagans DMTR-CTR-3044 (NMI Accession No. V22/006355), Thozetella nivea DMTR-CTR-2359 (NMI Accession No. V22/003496), Trichoderma hamatum DMTR-CTR-US-73 (ATCC Accession No. PTA-127301), Trichoderma longipile/spirale DMTR-CTR-1291 (NMI Accession No. V22/006354), and a combination thereof; and
the one or more fungal strains are inoculated in an amount effective to increase organic carbon in the soil compared to a non-inoculated control soil.
2. The method of claim 1 , further comprising an initial step of identifying the soil as having a soil organic carbon (SOC) level below 5%.
3. The method of claim 1 , wherein the soil and/or plant are non-native to the one or more fungal strains and the non-native plant is selected from the group consisting of sugar cane, wheat, rice, corn (maize), rye, oats, barley, sorghum, millet, flax, hemp, jute, cotton, soybeans, alfalfa, clover, peanuts, lentils, lupins, peas, and chickpea.
4. A method of enhancing plant growth, comprising:
applying to a plant, a plant part, or the locus surrounding the plant, one or more fungal strains selected from the group consisting of Acrocalymma vagum, Leptodontidium, Periconia, Phaeosphaeria, Thozetella, Trichoderma , and a combination thereof;
wherein the one or more fungal strains has a nuclear ribosomal internal transcribed spacer 2 (ITS2) sequence that is at least 90% identical to the nucleotide sequence of SEQ ID No: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or 19; and
the one or more fungal strains are applied in an amount effective to enhance the growth of the plant as compared to an untreated control plant.
5. The method of claim 4 , wherein the one or more fungal strains are from a species selected from the group consisting of Leptodontidium orchidicola, Periconia sp., Periconia macrospinosa, Phaeosphaeria luctuosa, Phaeosphaeria vagans, Thozetella nivea, Trichoderma hamatum, Trichoderma longipile, Trichoderma spirale , and a combination thereof.
6. The method of claim 5 , wherein the one or more fungal strains are selected from the group consisting of Acrocalymma vagum DMTR-CTR-11556 (NMI Accession No. V22/006357), Leptodontidium orchidicola DMTR-CTR- 4873 (NMI Accession No. V22/003497), Periconia sp. DMTR-CTR-6649 (NMI Accession No. V22/006356), Periconia macrospinosa DMTR-CTR-US-125 (ATCC Accession No. PTA-127300), Periconia macrospinosa DMTR-CTR-1852 (NMI Accession No. V22/006358), Phaeosphaeria luctuosa/vagans DMTR-CTR-3044 (NMI Accession No. V22/006355), Thozetella nivea DMTR-CTR-2359 (NMI Accession No. V22/003496), Trichoderma hamatum DMTR-CTR-US-73 (ATCC Accession No. PTA-127301), Trichoderma longipile/spirale DMTR-CTR-1291 (NMI Accession No. V22/006354), and a combination thereof.
7. The method of claim 4 , wherein the plant is non-native to the one or more fungal strains and the non-native plant is selected from the group consisting of sugar cane, wheat, rice, corn (maize), rye, oats, barley, sorghum, millet, flax, hemp, jute, cotton, soybeans, alfalfa, clover, peanuts, lentils, lupins, peas, and chickpea.
8. A method for sequestering atmospheric carbon for storage as organic carbon in a soil, comprising:
inoculating the soil and/or a plant growing in the soil with one or more fungal strains selected from the group consisting of Acrocalymma vagum, Clonostachys, Leptodontidium, Periconia, Phaeosphaeria, Thozetella, Trichoderma , and a combination thereof,
wherein the one or more fungal strains have a nuclear ribosomal internal transcribed spacer 2 (ITS2) sequence that is at least 90% identical to the nucleotide sequence of SEQ ID No: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or 19; and
the one or more fungal strains are inoculated in an amount effective to increase sequestered atmospheric carbon in the soil compared to a non-inoculated control soil.
9. The method of claim 8 , wherein the one or more fungal strains are selected from a species selected from the group consisting of Acrocalymma vagum, Clonostachys rosea, Leptodontidium orchidicola, Periconia sp., Periconia macrospinosa, Phaeosphaeria luctuosa, Phaeosphaeria vagans, Thozetella nivea, Trichoderma hamatum, Trichoderma longipile, Trichoderma spirale , and a combination thereof.
10. The method of claim 9 , wherein the one or more fungal strains are selected from the group consisting of Acrocalymma vagum DMTR-CTR-11556 (NMI Accession No. V22/006357), Leptodontidium orchidicola DMTR-CTR-4873 (NMI Accession No. V22/003497), Periconia sp. DMTR-CTR-6649 (NMI Accession No. V22/006356), Periconia macrospinosa DMTR-CTR-US-125 (ATCC Accession No. PTA-127300), Periconia macrospinosa DMTR-CTR-1852 (NMI Accession No. V22/006358), Phaeosphaeria luctuosa/vagans DMTR-CTR-3044 (NMI Accession No. V22/006355), Thozetella nivea DMTR-CTR-2359 (NMI Accession No. V22/003496), Trichoderma hamatum DMTR-CTR-US-73 (ATCC Accession No. PTA-127301), Trichoderma longipile/spirale DMTR-CTR-1291 (NMI Accession No. V22/006354), and a combination thereof.
11. The method of claim 10 , wherein the plant is a non- native plant selected from the group consisting of sugar cane, wheat, rice, corn (maize), rye, oats, barley, sorghum, millet, flax, hemp, jute, cotton, soybeans, alfalfa, clover, peanuts, lentils, lupins, peas, and chickpea.
12. A plant, plant part or plant seed associated with a composition comprising:
one or more isolated fungal strains from at least one genus selected from the group consisting of Acrocalymma vagum, Clonostachys, Leptodontidium, Periconia, Phaeosphaeria, Thozetella, Trichoderma , and a combination thereof; wherein the one or more isolated fungal strains has a nuclear ribosomal internal transcribed spacer 2 (ITS2) sequence that is at least 90% identical to the nucleotide sequence of SEQ ID No: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or 19; and
an agriculturally acceptable carrier;
wherein the composition is applied or coated on at least a portion of an outer surface of the plant, plant part or plant seed.
13. The plant, plant part or plant seed of claim 12 , wherein the plant is a non-native plant selected from the group consisting of sugar cane, wheat, rice, corn (maize), rye, oats, barley, sorghum, millet, flax, hemp, jute, cotton, soybeans, alfalfa, clover, peanuts, lentils, lupins, peas, and chickpea.
14. The plant, plant part or plant seed of claim 12 , wherein the agriculturally acceptable carrier is a mineral earth, a cellulose, or a starch.