Centrifuge
Centrifuges are useful to, among other things, remove red blood cells from whole blood and retain platelets and other factors in a reduced volume of plasma. Platelet rich plasma (PRP) and or platelet poor plasma (PPP) can be obtained rapidly and is ready for immediate injection into the host. Embodiments may include valves, operated manually or automatically, to open ports that discharge the excess red blood cells and the excess plasma into separate receivers while retaining the platelets and other factors in the centrifuge chamber. High speeds used allow simple and small embodiments to be used at the patient's side during surgical procedures. The embodiments can also be used for the separation of liquids or slurries in other fields such as, for example, the separation of pigments or lubricants.
1. A method of isolating and concentrating a fraction of a biologic liquid mixture, comprising the steps of:
a. providing a centrifuge comprising:
i. a chamber having a longitudinal axis, a first end, a second end, and a sidewall extending between said first end and said second end, wherein at least a portion of said sidewall is transparent;
ii. a first port provided in said chamber at a first radial distance from said longitudinal axis, and in fluid communication with a first valve;
iii. a second port provided in said chamber at a second radial distance from said longitudinal axis, and in fluid communication with a second valve, said second radial distance being less than said first radial distance; and
iv. a motor configured to rotate said chamber about said longitudinal axis and thereby produce a centrifugal field;
wherein the sidewall is configured to direct toward the first port a biologic liquid mixture that is present in the chamber and experiencing a centrifugal field;
b. introducing into the chamber a biologic liquid mixture that is separable into at least a first fraction and a second fraction under centrifugal field;
c. rotating the chamber about the longitudinal axis, thereby separating the biologic mixture into the first fraction and the second fraction;
d. ejecting at least a portion of the first fraction from the chamber through the first port and leaving a residual of the biologic liquid mixture in the chamber; and
e. ejecting at least a portion of the second fraction from the chamber through the second port after performing step d.
2. The method of claim 1 , wherein said biologic liquid mixture is blood and said separation of said fractions results in one or more of: at least a 6.3 platelet concentration factor; at least 87% platelet recovery; and at least 92% of red blood cells removed from said biologic liquid mixture.
3. The method of claim 1 , further comprising the step of:
f. collecting at least a portion of said biologic liquid mixture remaining within said chamber.
4. The method of claim 1 , further comprising between step d and step e, the step of monitoring an interface between said first fraction and said second fraction through said sidewall and executing step e in response to the position of the interface.
5. The method of claim 1 , further comprising between step d and step e, the step of detecting an interface occurring between separated constituents of said sample by at least one automatic detector and executing step e in response to a signal produced by the automatic detector.
6. The method of claim 1 , wherein step d comprises selectively opening the first valve.
7. The method of claim 1 , wherein step e comprises selectively opening the second valve.
8. The method of claim 7 , further comprising between step d and step e, the step of closing said first valve.
9. The method of claim 1 , wherein the sidewall extends from the first end to the second end in a first direction that is away from the longitudinal axis.
10. The method of claim 1 , wherein the sidewall extends in a first direction from the first end to the second end that is away from the longitudinal axis, and the sidewall extends in a second direction from the first end to the second end that is toward the longitudinal axis.
11. The method of claim 10 , wherein the first radial distance is between the portion of the sidewall that extends in the first direction and the portion of the sidewall that extends in the second direction.
12. The method of claim 1 , wherein the chamber is disk-shaped.
13. The method of claim 1 , wherein the first port comprises an annular passageway.
14. The method of claim 1 , wherein the first port is in fluid communication with a first reservoir and the second port is in fluid communication with a second reservoir.
15. A method of isolating and concentrating a fraction of a biologic liquid mixture, comprising the steps of:
a. providing a centrifuge comprising:
i. a chamber having a longitudinal axis, a first end, a second end, and a sidewall extending between said first end and said second end, wherein at least a portion of said sidewall is transparent;
ii. a first port provided in said chamber at a first radial distance from said longitudinal axis, and in fluid communication with a first valve;
iii. a second port provided in said chamber at a second radial distance from said longitudinal axis, and in fluid communication with a second valve, said second radial distance being less than said first radial distance; and
iv. a motor configured to rotate said chamber about said longitudinal axis and thereby produce a centrifugal field;
wherein the sidewall is configured to direct toward the first port a biologic liquid mixture that is present in the chamber and experiencing a centrifugal field;
b. introducing into the chamber a biologic liquid mixture that is separable into at least a first fraction and a second fraction under centrifugal field;
c. rotating the chamber about the longitudinal axis, thereby separating the biologic mixture into the first fraction and the second fraction;
d. ejecting at least a portion of the first fraction from the chamber through the first port by opening the first valve;
e. visually monitoring a position of an interface between said first fraction and said second fraction through said sidewall position of the interface, and
f. after the interface reaches a predetermined location, ejecting at least a portion of the second fraction from the chamber through the second port by opening the second valve.
16. The method of claim 15 , wherein the biologic liquid mixture is blood.
17. The method of claim 15 , wherein step e further comprises closing the first valve when the position of the interface reaches the predetermined location.
18. The method of claim 15 , wherein step f results in a portion of the biologic liquid mixture remaining in the chamber.
19. The method of claim 18 , wherein the portion of the biologic liquid mixture remaining in the chamber does not comprise either the first fraction or the second fraction.
20. The method of claim 18 , further comprising the step of:
g. collecting at least a portion of said sample remaining within said chamber.