IP Library Granted Patent US 12,357,571
Granted Patent B2
US 12,357,571 · App. 18/045,727 · Granted Jul 15, 2025

Loading of extracellular vesicles through imparting of mechanical shear

Inventors: Aaron R. Noyes (Melrose, MA); Michael P. Mercaldi (Wilmington, MA); Kathryn E. Golden (Braintree, MA); Raymond W. Bourdeau (Watertown, MA); Michael F. Doherty (Somerville, MA); Konstantin Konstantinov (Waban, MA); Douglas E. Williams (Boston, MA)
Assignee: LONZA SALES AG
A61K9/1278A61K9/1275C12N15/111C12N15/113C12N2310/14C12N2310/3515C12N2320/32
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Quick Facts
Patent No.
US 12,357,571
App. No.
18/045,727
Granted
Jul 15, 2025
Kind
B2
Abstract

Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.

Claims (18)

1. A method for producing an isolated exosome for delivery of a payload molecule, the method comprising:

modifying an exosome with the payload molecule via homogenization,

isolating the modified exosome containing the payload molecule, and

optionally formulating the isolated modified exosome into a pharmaceutical composition,

wherein the homogenization is microfluidization,

wherein the microfluidization comprises multiple passes,

wherein each of the multiple passes is performed at between 10,000 and 30,000 psi, and

wherein the exosome is in buffered solution and the buffered solution comprises 0.5-5% w/v sucrose.

2. A pharmaceutical composition comprising a plurality of exosomes produced according to the method of claim 1 , and a pharmaceutically-acceptable excipient or carrier in a form suitable for administration to a subject.

3. The method of claim 1 , wherein the microfluidization comprises three or more passes at between 20,000 and 30,000 psi.

4. The method of claim 1 , wherein the microfluidization comprises three or more passes at 30,000 psi.

5. The method of claim 1 , wherein the microfluidization comprises a first pass at 10,000 psi and a second pass at 30,000 psi.

6. The method of claim 1 , wherein the microfluidization comprises a first pass at 20,000 psi and a second pass at 30,000 psi.

7. The method of claim 1 , wherein the microfluidization comprises five or more passes.

8. The method of claim 1 , wherein the microfluidization comprises five or more passes at 30,000 psi.

9. The method of claim 1 , wherein the payload molecule is siRNA, miRNA, antisense RNA, DNA, a plasmid, mRNA, tRNA, a protein, a carbohydrate, a lipid, a small molecule drug, a toxin, an antibody, a recombinant protein, a viral vector, or a vaccine.

10. The method of claim 9 , wherein the payload molecule is siRNA.

11. The method of claim 9 , wherein the payload molecule is a modified siRNA or miRNA that is modified to additionally comprise one or more cholesterol molecules.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 31, 2023
From: NOYES, AARON R.; MERCALDI, MICHAEL P.; GOLDEN, KATHRYN E.; BOURDEAU, RAYMOND W.; DOHERTY, MICHAEL F.; KONSTANTINOV, KONSTANTIN; WILLIAMS, DOUGLAS E.
To: CODIAK BIOSCIENCES, INC.
Reel/Frame 064765/0550 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 11, 2023
From: CODIAK BIOSCIENCES, INC.
To: LONZA SALES AG
Reel/Frame 064251/0794 →
Continuity (4)
Continuation 17014961 · Sep 8, 2020
Continuation 16190058 · Nov 13, 2018
Provisional Application 62588143 · Nov 17, 2017
Related Publication 20230301917A1 · Sep 28, 2023
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