IP Library Granted Patent US 12,371,654
Granted Patent B2
US 12,371,654 · App. 18/133,402 · Granted Jul 29, 2025

Biological and algae harvesting and cultivation systems and methods

Inventors: David A. Hazlebeck (El Cajon, CA); William Rickman (Lebanon, TN)
Assignee: Global Algae Technology, LLC
C12N1/12B01D61/22B01D63/02B01D63/04B01D63/046B01D65/02C12M21/02C12M29/04C12M29/16C12M29/18C12M29/20C12M33/14C12M41/32C12M41/44C12M41/48C12M45/00C12M47/02C12N1/02B01D2311/04B01D2311/06B01D2311/2626B01D2311/2688B01D2313/18B01D2313/26B01D2313/501B01D2315/06B01D2315/08B01D2317/02B01D2317/022B01D2321/04B01D2321/18B01D2321/185B01D2321/40
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,371,654
App. No.
18/133,402
Granted
Jul 29, 2025
Kind
B2
Abstract

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

Claims (38)

1. An algae harvesting and cultivation system comprising:

a first algae cultivator defining a first cultivation stage, the first cultivation stage including a first at least one cultivation device having a first algae cultivation media for cultivating algae;

a first separation system in fluid communication with the first cultivation stage such that the first separation system receives at least a portion of the algae cultivated from the first cultivation stage, the first separation system configured to separate the algae received from the first cultivation stage so as to produce a first algae slurry and a first substantially algae-free media;

a first carbon dioxide adder configured to add carbon dioxide only from air to the first substantially algae-free media prior to the first substantially algae-free media flowing to the first cultivation stage;

wherein the system is constructed and arranged such that the first substantially algae-free media flows back to the first cultivation stage;

a second algae cultivator defining a second cultivation stage, the second cultivation stage in fluid communication with the first separation system such that the second cultivation stage receives the first algae slurry, the second cultivation stage including a second at least one cultivation device having a second media for cultivating additional algae using the first algae slurry;

a second separation system in fluid communication with the second cultivation stage such that the second separation system receives at least a portion of said additional algae cultivated in the second cultivation stage, the second separation system configured to separate the additional algae received from the second cultivation stage so as to produce a second substantially algae-free media and a second algae slurry; and

a second carbon dioxide adder configured to add carbon dioxide only from air to the second substantially algae-free media prior to the second substantially algae-free media flowing to the second cultivation stage;

wherein the system is further constructed and arranged such that the second substantially algae-free media flows back to the second cultivation stage.

2. The algae harvesting and cultivation system of claim 1 , wherein (i) the first carbon dioxide adder includes a first gas-liquid contactor that enables the first substantially algae-free media to absorb carbon dioxide from the air and convert carbonate ions in the first substantially algae-free media to bicarbonate ions; and (ii) the second carbon dioxide adder includes a second gas-liquid contactor that enables the second substantially algae-free media to absorb carbon dioxide from the air and convert carbonate ions in the second substantially algae-free media to bicarbonate ions.

3. The algae harvesting and cultivation system of claim 2 , wherein at least one of the first gas-liquid contactor or the second gas-liquid contactor is a carbon dioxide absorber.

4. The algae harvesting and cultivation system of claim 2 , wherein the first gas-liquid contactor is in fluid communication with a first holding reservoir so as to receive the first substantially algae-free media from the first holding reservoir prior to the first substantially algae-free media flowing back to the first cultivation stage.

5. The algae harvesting and cultivation system of claim 4 , wherein the second gas-liquid contactor is in fluid communication with a second holding reservoir so as to receive the second substantially algae-free media from the second holding reservoir prior to the second substantially algae-free media flowing back to the second cultivation stage.

6. The algae harvesting and cultivation system of claim 5 , wherein the first gas-liquid contactor includes a gas stream inlet for receiving the air, and wherein the second gas-liquid contactor includes a gas stream inlet for receiving the air.

7. The algae harvesting and cultivation system of claim 5 , further comprising:

a third holding reservoir constructed and arranged to receive the first substantially algae-free media from the first gas-liquid contactor, and arranged such that the first substantially algae-free media flows from the third holding reservoir back to the first cultivation stage.

8. The algae harvesting and cultivation system of claim 7 , wherein the third holding reservoir includes a carbon dioxide retaining cover.

9. The algae harvesting and cultivation system of claim 7 , further comprising:

a fourth holding reservoir constructed and arranged to receive the second substantially algae-free media from the second gas-liquid contactor, wherein the system is constructed and arranged such that the second substantially algae-free media can flow from the fourth holding reservoir back to the second cultivation stage.

10. The algae harvesting and cultivation system of claim 2 further comprising: a first holding reservoir constructed and arranged to receive the first substantially algae-free media, arranged such that the first gas-liquid contactor is connected to or held within the first holding reservoir, and arranged such that the first substantially algae-free media flows from the first holding reservoir back to the first cultivation stage.

11. The algae harvesting and cultivation system of claim 10 further comprising: a second holding reservoir constructed and arranged to receive the second substantially algae-free media, arranged such that the second gas-liquid contactor is connected to or held within the second holding reservoir, and arranged such that the second substantially algae-free media flows from the second holding reservoir back to the second cultivation stage.

12. The algae harvesting and cultivation system of claim 1 , wherein the first separation system includes hollow fiber membranes to filter the first algae slurry from the first algae cultivation media.

13. An algae harvesting and cultivation system comprising:

a first algae cultivator including a first at least one cultivation device having a first algae cultivation media for cultivating algae;

a first separation system in fluid communication with the first at least one cultivation device such that the first separation system receives at least a portion of the algae cultivated from the first at least one cultivation device, the first separation system configured to separate the algae received from the first at least one cultivation device so as to produce a first algae slurry and a first substantially algae-free media; and

a first carbon dioxide adder configured to add carbon dioxide only from air to the first substantially algae-free media prior to the first substantially algae-free media flowing to the first at least one cultivation device;

wherein the system is constructed and arranged such that the first substantially algae-free media flows back to the first at least one cultivation device.

14. The algae harvesting and cultivation system of claim 13 , wherein the first carbon dioxide adder includes a first gas-liquid contactor that enables the first substantially algae-free media to absorb carbon dioxide from the air and convert carbonate ions in the first substantially algae-free media to bicarbonate ions.

15. The algae harvesting and cultivation system of claim 14 , wherein the first gas-liquid contactor is a first carbon dioxide absorber.

16. The algae harvesting and cultivation system of claim 15 , wherein the first gas-liquid contactor is in fluid communication with a first holding reservoir so as to receive the first substantially algae-free media from the first holding reservoir prior to the first substantially algae-free media flowing back to the first at least one cultivation device.

17. The algae harvesting and cultivation system of claim 16 , further comprising:

an additional holding reservoir constructed and arranged to receive the first substantially algae-free media from the first gas-liquid contactor, wherein the system is constructed and arranged such that the first substantially algae-free media flows from the additional holding reservoir back to the first at least one cultivation device.

18. The algae harvesting and cultivation system of claim 14 further comprising: a first holding reservoir constructed and arranged to receive the first substantially algae-free media, arranged such that the first gas-liquid contactor is connected to or held within the first holding reservoir, and arranged such that the first substantially algae-free media flows from the first holding reservoir back to the first at least one cultivation device.

19. The algae harvesting and cultivation system of claim 13 , wherein the first algae cultivator defines a first cultivation stage, the system further comprising:

a second algae cultivator defining a second cultivation stage, the second cultivation stage in fluid communication with the first separation system such that the second cultivation stage receives the first algae slurry, the second cultivation stage including a second at least one cultivation device having a second media for cultivating additional algae using the first algae slurry;

a second separation system in fluid communication with the second cultivation stage such that the second separation system receives at least a portion of said additional algae cultivated in the second cultivation stage, the second separation system configured to separate the additional algae received from the second cultivation stage so as to produce a second substantially algae-free media and a second algae slurry; and

a second carbon dioxide adder configured to add carbon dioxide only from air to the second substantially algae-free media prior to the second substantially algae-free media flowing to the second cultivation stage, the second carbon dioxide adder including a second carbon dioxide absorber that enables the second substantially algae-free media to absorb carbon dioxide only from air and convert carbonate ions in the second substantially algae-free media to bicarbonate ions and a second packed bed absorption column in fluid communication with a second holding reservoir.

20. The algae harvesting and cultivation system of claim 13 , wherein the first separation system includes hollow fiber membranes to filter the first algae slurry from the first algae cultivation media.

Continuity (10)
Continuation 16853549 · Apr 20, 2020
Division 15273552 · Sep 22, 2016
Provisional Application 62333674 · May 9, 2016
Provisional Application 62333681 · May 9, 2016
Provisional Application 62333688 · May 9, 2016
Provisional Application 62333691 · May 9, 2016
Provisional Application 62333696 · May 9, 2016
Provisional Application 62333702 · May 9, 2016
Provisional Application 62333705 · May 9, 2016
Related Publication 20230242865A1 · Aug 3, 2023
References Cited (148)
US 2658310A · Cook · 1953 [cited by applicant]
US 2732661A · Spoehr et al. · 1956 [cited by applicant]
US 3780471A · Ort · 1973 [cited by applicant]
US 3958364A · Schenck et al. · 1976 [cited by applicant]
US 3969844A · Fogel et al. · 1976 [cited by applicant]
US 4087936A · Savins et al. · 1978 [cited by applicant]
US 4115949A · Avron et al. · 1978 [cited by applicant]
US 4199895A · Avron et al. · 1980 [cited by applicant]
US 4236349A · Ramus · 1980 [cited by applicant]
US 4253271A · Raymond · 1981 [cited by applicant]
US 4267038A · Thompson · 1981 [cited by applicant]
US 4324067A · Kessler · 1982 [cited by applicant]
US 4341038A · Bloch et al. · 1982 [cited by applicant]
US 4438591A · Kessler · 1984 [cited by applicant]
US 4473970A · Hills · 1984 [cited by applicant]
US 4767539A · Ford · 1988 [cited by applicant]
US 4876006A · Ohkubo et al. · 1989 [cited by applicant]
US 5151191A · Sunaoka et al. · 1992 [cited by applicant]
US 5166067A · Ishida et al. · 1992 [cited by applicant]
US 5248424A · Cote et al. · 1993 [cited by applicant]
US 5288399A · Schulz · 1994 [cited by applicant]
US 5393433A · Espenan et al. · 1995 [cited by applicant]
US 5403479A · Smith et al. · 1995 [cited by applicant]
US 5480533A · Yoshida · 1996 [cited by applicant]
US 5541056A · Huntley et al. · 1996 [cited by applicant]
US 5639373A · Mahendran et al. · 1997 [cited by applicant]
US 5783083A · Henshaw et al. · 1998 [cited by applicant]
US 5944997A · Pedersen et al. · 1999 [cited by applicant]
US 5958243A · Lawrence et al. · 1999 [cited by applicant]
US 6027649A · Benedek et al. · 2000 [cited by applicant]
US 6120688A · Daly et al. · 2000 [cited by applicant]
US 6156200A · Zha et al. · 2000 [cited by applicant]
US 6193890B1 · Pedersen et al. · 2001 [cited by applicant]
US 6214231B1 · Cote et al. · 2001 [cited by applicant]
US 6245239B1 · Cote et al. · 2001 [cited by applicant]
US 6303035B1 · Cote et al. · 2001 [cited by applicant]
US 6319411B1 · Cote · 2001 [cited by applicant]
US 6375848B1 · Cote et al. · 2002 [cited by applicant]
US 6547968B1 · Rabie et al. · 2003 [cited by applicant]
US 6550747B2 · Rabie et al. · 2003 [cited by applicant]
US 6616843B1 · Behmann et al. · 2003 [cited by applicant]
US 6682652B2 · Mahendran et al. · 2004 [cited by applicant]
US 6706189B2 · Rabie et al. · 2004 [cited by applicant]
US 6814861B2 · Husain et al. · 2004 [cited by applicant]
US 6881343B2 · Rabie et al. · 2005 [cited by applicant]
US 6899812B2 · Cote et al. · 2005 [cited by applicant]
US 6964741B2 · Mahendran et al. · 2005 [cited by applicant]
US 7014173B2 · Rabie et al. · 2006 [cited by applicant]
US 7025885B2 · Cote et al. · 2006 [cited by applicant]
US 7063788B2 · Mahendran et al. · 2006 [cited by applicant]
US 7122121B1 · Ji · 2006 [cited by applicant]
US 7186343B2 · Rabie et al. · 2007 [cited by applicant]
US 7198721B2 · Cote et al. · 2007 [cited by applicant]
US 7378024B2 · Bartels et al. · 2008 [cited by applicant]
US 7476322B2 · Dimitriou et al. · 2009 [cited by applicant]
US 7625157B2 · Prichard et al. · 2009 [cited by applicant]
US 7687261B2 · Hazlebeck et al. · 2010 [cited by applicant]
US 7820050B2 · Cote et al. · 2010 [cited by applicant]
US 7879229B2 · Phagoo et al. · 2011 [cited by applicant]
US 7922910B2 · Cote et al. · 2011 [cited by applicant]
US 8114293B2 · Phagoo et al. · 2012 [cited by applicant]
US 8652331B2 · Zha et al. · 2014 [cited by applicant]
US 8926844B2 · Parsheh et al. · 2015 [cited by applicant]
US 9181523B1 · Ganuza et al. · 2015 [cited by applicant]
US 9894856B2 · Javan et al. · 2018 [cited by applicant]
US 10123495B2 · Ordway et al. · 2018 [cited by applicant]
US 10501721B2 · Hazlebeck et al. · 2019 [cited by applicant]
US 20020034817A1 · Henry et al. · 2002 [cited by applicant]
US 20030042184A1 · McGowan · 2003 [cited by applicant]
US 20050061725A1 · Liu et al. · 2005 [cited by applicant]
US 20050082227A1 · Cote et al. · 2005 [cited by applicant]
US 20050161388A1 · Williams et al. · 2005 [cited by applicant]
US 20060008865A1 · Cote · 2006 [cited by applicant]
US 20070039888A1 · Ginzburg et al. · 2007 [cited by applicant]
US 20070048859A1 · Sears · 2007 [cited by applicant]
US 20070075021A1 · Johnson · 2007 [cited by applicant]
US 20070138070A1 · Dimitriou et al. · 2007 [cited by applicant]
US 20080009055A1 · Lewnard · 2008 [cited by applicant]
US 20080086937A1 · Hazlebeck et al. · 2008 [cited by applicant]
US 20080086938A1 · Hazlebeck et al. · 2008 [cited by applicant]
US 20080160593A1 · Oyler · 2008 [cited by applicant]
US 20090104098A1 · Singh · 2009 [cited by applicant]
US 20090166276A1 · Abe et al. · 2009 [cited by applicant]
US 20090298159A1 · Wu et al. · 2009 [cited by applicant]
US 20100162620A1 · McCaffrey et al. · 2010 [cited by applicant]
US 20100190227A1 · Dauth et al. · 2010 [cited by applicant]
US 20100236137A1 · Wu et al. · 2010 [cited by applicant]
US 20110049038A1 · Aerts et al. · 2011 [cited by applicant]
US 20110139715A1 · Zha et al. · 2011 [cited by applicant]
US 20110247977A1 · Song et al. · 2011 [cited by applicant]
US 20110309038A1 · Inoue · 2011 [cited by applicant]
US 20120094361A1 · Hu et al. · 2012 [cited by applicant]
US 20120125846A1 · Suzumura et al. · 2012 [cited by applicant]
US 20120231528A1 · Muller-Feuga et al. · 2012 [cited by applicant]
US 20130146548A1 · Cote · 2013 [cited by applicant]
US 20130213887A1 · Morikawa et al. · 2013 [cited by applicant]
US 20130217082A1 · Hazlebeck · 2013 [cited by applicant]
US 20130228227A1 · Kempson et al. · 2013 [cited by applicant]
US 20140042074A1 · Noh et al. · 2014 [cited by applicant]
US 20140065701A1 · Kabakian · 2014 [cited by applicant]
US 20140206072A1 · Severino Do Rosario De Quintanilha Dos Santos et al. · 2014 [cited by applicant]
US 20140259896A1 · Oney · 2014 [cited by applicant]
US 20150315534A1 · Vargas, Jr. et al. · 2015 [cited by applicant]
US 20150353396A1 · Takabatake et al. · 2015 [cited by applicant]
US 20180093908A1 · Chidambaran et al. · 2018 [cited by applicant]
US 20180221825A1 · Imamura · 2018 [cited by applicant]
CN 103789195A · 2014 [cited by applicant]
EP 0336966A1 · 1989 [cited by applicant]
JP H0623245A · 1994 [cited by applicant]
JP 2013027378A · 2013 [cited by applicant]
WO 2014159439A1 · 2014 [cited by applicant]
M.R. Bilad et al. / Bioresource Technology 155 (2014) 410-417. (Year: 2014). [cited by examiner]
M.R. Bilad et al. / Biotechnology Advances 32 (2014) 1283-1300. (Year: 2014). [cited by examiner]
Extended European Search Report, EP16901893.4, dated Feb. 11, 2020, 4 pages. [cited by applicant]
International Search Report and Written Opinion, International Application No. PCT/US2016/053203, dated Jan. 30, 2017. [cited by applicant]
International Searching Authority Invitation to Pay Additional Fees for related International Application No. PCT/US2016/053203 dated Nov. 16, 2016. [cited by applicant]
Akhondi et al., Evaluation of Fouling Deposition, Fouling Reversibility and Energy Consumption of Submerged Hollow Fiber Membrane Systems with Periodic Backwash, J. Membr. Sci. 452 (2014) pp. 319-333. [cited by applicant]
Albasi et al., Filtration of Biological Sludge by Immersed Hollow-Fiber Membranes: Influence of Initial Permeability Choice of Operating Conditions, Desalination 146 (2002) pp. 427-431. [cited by applicant]
Bhave et al., Membrane-Based Energy Efficient Dewatering of Microalgae in Biofuels Production and Recovery of Value Added Co-Products, Environ. Sci. Technol. 46 (2012) pp. 5599-5606. [cited by applicant]
Bohutskyi et al. Mineral and non-carbon nutrient utilization and recovery during sequential phototrophic-heterotrophic growth of lipid-rich algae. Appl Microbial Biotechnol (2014) 98:5261-5273. (Year: 2014). [cited by applicant]
Buzatu et al., Permeability and Clogging in an Immersed Hollow Fibre Membrane Bioreactor, J. Membr. Sci. 421-422 2012) pp. 342-348. [cited by applicant]
Current Environmental Issues and Challenges; G. Cao; Roberto Orru, Dept. of Mechanical, Chemical and Materials Engineering, University of Cagliari, Caliari, Italy; Springer Science+Business Media Dordrecht (2014). [cited by applicant]
Danquah et al., Microalgal Growth Characteristics and Subsequent Influence on Dewatering Efficiency, Chem. Eng. J. 151 (2009) pp. 73-78. [cited by applicant]
Entech, Control Valve Dynamic Specification, Ver. 3.0, Nov. 1998. [cited by applicant]
Hendricks, Waler Treatment Unit Processes, CRC Press, Boca Ralon (2011) pp. 539, 560. [cited by applicant]
Hillis et al., Effects of Backwash Conditions on Out-lo-in Membrane Microfillralion, Desalination 118 (1998) pp. 197-204. [cited by applicant]
Itokowa et al., Design and Operating Experiences of Municipal MBRS in Europe, Waler Sci. Technol. 58(12) (2008) p. 2319-2327. [cited by applicant]
Ivanovic et al., Impact of Aeration Rates on Particle Colloidal Fraction in the Biofilm Membrane Bioreactor (BF-MBR), Desalination 231 (2008) pp. 182-190. [cited by applicant]
Jiang et al., Optimising the Operation of a MBR Pilot Plant by Quantitative Analysis of the Membrane Fouling Mechanism, Waler Sci. Technol. 51 No. 6-7 (2005) pp. 19-25. [cited by applicant]
Katuri et al., A Novel Anaerobic Electrochemical Membrane Bioreactor (AnEMBR) With Conductive Hollow-fiber Membrane for Treatment of Low-Organic Strength Solutions, Environ. Sci. Technol. 48 (2014) pp. 12833-12841. [cited by applicant]
Khirani et al., Effect of Periodic Backwash in the Submerged Membrane Adsorption Hybrid System (SMAHS) for Wastewater Treatment, Desalination 191 (2006) pp. 27-34. [cited by applicant]
Lee et al., Chilosan Coagulalion—Membrane Filtration of Chlorella vulgaris, Inl'l J. Hydrogen Energy 37 (2012) pp. 15643-15647. [cited by applicant]
Pinnekamp et al., Design and Operation of Membrane Bioreactors in Europe (2012). [cited by applicant]
Prasenjit Mondall; Ajay K. Dalai; Sustainable Utilization of Natural Resources; (2017) Taylor & Francis Group, LLC, Boca Raton, Florida, US. [cited by applicant]
Raffin et al., Influence of Backwashing, Flux and Temperature on Microfiltration for Wastewater Reuse, Sep. Purif. Technol. 96 (2012) pp. 147-153. [cited by applicant]
Schoeberl et al., Optimization of Operational Parameters for a Submerged Membrane Bioreactor Treating Dyehouse Wastewater, Sep. Purif.Technol. 44 (2005) pp. 61-68. [cited by applicant]
Serra et al., Use of Air Sparging to Improve Backwash Efficiency in Hollow-Fiber Modules, J. Membr. Sci. 161 (1999) pp. 95-113. [cited by applicant]
Smith et al., Design of a Genetic Control System for Optimising Back Flush Durations in a Submerged Membrane Hybrid Reactor, J. Membr. Sci. (2005) pp. 99-106. [cited by applicant]
Smith et al., A New Approach to Backwash Initiation in Membrane Systems, J. Membr. Sci. 278 (2006) pp. 381-389. [cited by applicant]
Wu et al., Effects of Relaxation and Backwashing Conditions on Fouling in Membrane Bioreactor, J. Membr. Sci. 324 (2008) pp. 26-32. [cited by applicant]
Wu et al., Novel Filtration Mode for Fouling Limitation in Membrane Bioreactors, Waler Res. 42 (2008) pp. 3677-3684. [cited by applicant]
Yigit et al., Effects of Various Backwash Scenarios on Membrane Fouling in a Membrane Bioreactor, Desalination 237 (2009) pp. 346-356. [cited by applicant]
Zsirai et al., Efficacy of Relation, Backflushing, Chemical Cleaning and Clogging Removal for an Immersed Hollow Fibre Membrane Bioreactor, Waler Res. 46 (2012) pp. 4499-4507. [cited by applicant]
Extended European Search Report of EP Application No. 21202229.7, mailed Feb. 2, 2022, 8 pages. [cited by applicant]
International Search Report of International Application No. PCT/US21/36608, mailed Sep. 15, 2021, 2 pages. [cited by applicant]
Written Opinion of International Application No. PCT/US21/36608, mailed Sep. 15, 2021, 3 pages. [cited by applicant]
English language machine translation of CN103789195, 4 pages, no date. [cited by applicant]
Srijaroonrat et al., “Unstable secondary oil/water emulsion treatment using ultrfiltrationL fouling control by backflushing”, Journal of Membrane Science, 159 (1999) 11-20. [cited by applicant]
Cited By (1)
US 12,544,693