Granted Patent
B2
US 12,404,306 · App. 16/978,573 · Granted Sep 2, 2025
Human papillomavirus vaccines and uses of the same
Inventors:
Douglas E. Brough (Germantown, MD); Cheryl G. Bolinger (Germantown, MD); Ramya Yarlagadda (Blacksburg, VA); Vinodhbabu Kurella (Blacksburg, VA); Prabakaran Ponraj (Blacksburg, VA); Simon Metenou (Germantown, MD); Kuan-Fu Ding (Blacksburg, VA)
Assignee:
Precigen, Inc.
C07K14/005A61K39/12A61K39/292A61K48/0066C07K14/02C12N7/00C12N15/861C07K2319/33C12N2710/20022C12N2710/20034
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Abstract
Provided herein are engineered human papilloma virus (HPV) molecular vaccine constructs. Vaccine constructs can also include ligand-inducible engineered gene switch systems for modulating expression of heterologous genes, such as a cytokines, in host cells.
Claims (12)
1. A polynucleotide encoding a polypeptide construct comprising the amino acid sequence of SEQ ID NO: 52 or 54.
2. A vector comprising the polynucleotide of claim 1 .
3. The vector of claim 2 , wherein the vector is an adenoviral vector.
4. The polynucleotide of claim 1 , wherein the polypeptide construct comprises the amino acid sequence of SEQ ID NO: 54.
5. The polynucleotide of claim 1 , wherein the polypeptide construct comprises the amino acid sequence of SEQ ID NO: 52.
6. The vector of claim 3 , wherein the adenoviral vector is a gorilla adenoviral vector.
7. The vector of claim 6 , wherein the gorilla adenoviral vector does not comprise an E1 region.
8. The vector of claim 6 , wherein the gorilla adenoviral vector does not comprise an E4 region.
9. The vector of claim 6 , wherein the gorilla adenoviral vector does not comprise an E1 region or an E4 region.
10. The vector of claim 6 , wherein the gorilla adenoviral vector is a GC46 vector.
11. The vector of claim 6 , wherein the polynucleotide is under the control of an immediate early cytomegalovirus (CMV) promoter.
12. A method of vaccinating a subject against human papilloma virus, the method comprising administering to the subject the vector of claim 6 .
Assignments (3)
PATENT SECURITY AGREEMENT
Recorded Sep 3, 2025
From: PRECIGEN, INC.; GENVEC LLC; PRECIGEN ACTOBIO, INC.; EXEMPLAR GENETICS, LLC
To: BIOPHARMA CREDIT PLC, AS COLLATERAL AGENT
Reel/Frame 072828/0564 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 11, 2022
From: PGEN THERAPEUTICS, INC.
To: PRECIGEN, INC.
Reel/Frame 061733/0843 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 16, 2021
From: BROUGH, DOUGLAS E.; BOLINGER, CHERYL G.; YARLAGADDA, RAMYA; KURELLA, VINODHBABU; PONRAJ, PRABAKARAN; METENOU, SIMON; DING, KUAN-FU
To: PRECIGEN, INC.
Reel/Frame 058119/0766 →
Continuity (2)
Provisional Application
62639354
· Mar 6, 2018
References Cited (233)
US 4985461A
· Hsu et al.
· 1991
[cited by applicant]
US 5117057A
· Hsu et al.
· 1992
[cited by applicant]
US 5122464A
· Wilson et al.
· 1992
[cited by applicant]
US 5225443A
· Murphy et al.
· 1993
[cited by applicant]
US 5283173A
· Fields et al.
· 1994
[cited by applicant]
US 5350674A
· Boenisch et al.
· 1994
[cited by applicant]
US 5378726A
· Yanagi et al.
· 1995
[cited by applicant]
US 5530028A
· Lidert et al.
· 1996
[cited by applicant]
US 5585362A
· Wilson et al.
· 1996
[cited by applicant]
US 5770359A
· Wilson et al.
· 1998
[cited by applicant]
US 5837511A
· Falck-Pedersen et al.
· 1998
[cited by applicant]
US 5851806A
· Kovesdi et al.
· 1998
[cited by applicant]
US 5880333A
· Goff et al.
· 1999
[cited by applicant]
US 5885827A
· Wabl et al.
· 1999
[cited by applicant]
US 5994106A
· Kovesdi et al.
· 1999
[cited by applicant]
US 5994128A
· Fallaux et al.
· 1999
[cited by applicant]
US 5998205A
· Hallenbeck et al.
· 1999
[cited by applicant]
US 6013836A
· Hsu et al.
· 2000
[cited by applicant]
US 6033908A
· Bout et al.
· 2000
[cited by applicant]
US 6127175A
· Vigne et al.
· 2000
[cited by applicant]
US 6225289B1
· Kovesdi et al.
· 2001
[cited by applicant]
US 6265173B1
· Evans et al.
· 2001
[cited by applicant]
US 6482616B1
· Kovesdi et al.
· 2002
[cited by applicant]
US 6489458B2
· Hackett et al.
· 2002
[cited by applicant]
US 6514943B2
· Kovesdi et al.
· 2003
[cited by applicant]
US 6613752B2
· Kay et al.
· 2003
[cited by applicant]
US 6677156B2
· Brough et al.
· 2004
[cited by applicant]
US 6682929B2
· Brough et al.
· 2004
[cited by applicant]
US 7091038B2
· Palli et al.
· 2006
[cited by applicant]
US 7148203B2
· Hackett et al.
· 2006
[cited by applicant]
US 7195896B2
· Kovesdi et al.
· 2007
[cited by applicant]
US 7531326B2
· Kapitskaya et al.
· 2009
[cited by applicant]
US 7563879B2
· Palli
· 2009
[cited by applicant]
US 7601508B2
· Palli et al.
· 2009
[cited by applicant]
US 7776587B2
· Palli et al.
· 2010
[cited by applicant]
US 7807417B2
· Palli et al.
· 2010
[cited by applicant]
US 7829676B2
· Zhang et al.
· 2010
[cited by applicant]
US 7919269B2
· Zhang et al.
· 2011
[cited by applicant]
US 7935510B2
· Palli et al.
· 2011
[cited by applicant]
US 7985739B2
· Kay et al.
· 2011
[cited by applicant]
US 8021878B2
· Palli
· 2011
[cited by applicant]
US 8030067B2
· Zhang et al.
· 2011
[cited by applicant]
US 8076454B2
· Palli et al.
· 2011
[cited by applicant]
US 8076517B2
· Hormann et al.
· 2011
[cited by applicant]
US 8105825B2
· Dhadialla et al.
· 2012
[cited by applicant]
US 8168426B2
· Dhadialla et al.
· 2012
[cited by applicant]
US 8202718B2
· Palli et al.
· 2012
[cited by applicant]
US 8227432B2
· Hackett et al.
· 2012
[cited by applicant]
US 8236556B2
· Kapitskaya et al.
· 2012
[cited by applicant]
US 8497093B2
· Palli
· 2013
[cited by applicant]
US 8598409B2
· Kapitskaya et al.
· 2013
[cited by applicant]
US 8603950B2
· Bowers et al.
· 2013
[cited by applicant]
US 8715959B2
· Palli et al.
· 2014
[cited by applicant]
US 9228180B2
· Izsvak et al.
· 2016
[cited by applicant]
US 9249207B2
· Palli et al.
· 2016
[cited by applicant]
US 9402919B2
· Roeth et al.
· 2016
[cited by applicant]
US 9492482B2
· Beech et al.
· 2016
[cited by applicant]
US 9493540B2
· Palli et al.
· 2016
[cited by applicant]
US 20050287161A1
· Dubin et al.
· 2005
[cited by applicant]
US 20070203326A1
· Dedhar et al.
· 2007
[cited by applicant]
US 20080233650A1
· Gall et al.
· 2008
[cited by applicant]
US 20090123441A1
· Braughler et al.
· 2009
[cited by applicant]
US 20090136465A1
· Merenick et al.
· 2009
[cited by applicant]
US 20130195800A1
· Roeth et al.
· 2013
[cited by applicant]
US 20160208285A1
· Roeth et al.
· 2016
[cited by applicant]
EP 0234994B1
· 1991
[cited by applicant]
EP 0461809B1
· 1994
[cited by applicant]
WO WO9208796A1
· 1992
[cited by applicant]
WO WO9428143A1
· 1994
[cited by applicant]
WO WO9428152A1
· 1994
[cited by applicant]
WO WO9502697A1
· 1995
[cited by applicant]
WO WO9516772A1
· 1995
[cited by applicant]
WO WO9534671A1
· 1995
[cited by applicant]
WO WO9622378A1
· 1996
[cited by applicant]
WO WO9700326A1
· 1997
[cited by applicant]
WO 1997005164A1
· 1997
[cited by applicant]
WO WO9712986A2
· 1997
[cited by applicant]
WO WO9721826A2
· 1997
[cited by applicant]
WO WO9738117A1
· 1997
[cited by applicant]
WO WO9902683A1
· 1999
[cited by applicant]
WO WO9958155A1
· 1999
[cited by applicant]
WO WO0000628B1
· 2000
[cited by applicant]
WO WO0034444A2
· 2000
[cited by applicant]
WO WO0151528A1
· 2001
[cited by applicant]
WO WO0170816A2
· 2001
[cited by applicant]
WO WO0198333A2
· 2001
[cited by applicant]
WO WO0229075A2
· 2002
[cited by applicant]
WO WO02066612A2
· 2002
[cited by applicant]
WO WO02066613A2
· 2002
[cited by applicant]
WO WO02066614A2
· 2002
[cited by applicant]
WO WO02066615A2
· 2002
[cited by applicant]
WO WO03020879A2
· 2003
[cited by applicant]
WO WO03022311A1
· 2003
[cited by applicant]
WO WO03027266A2
· 2003
[cited by applicant]
WO WO03027289A1
· 2003
[cited by applicant]
WO WO2005108617A2
· 2005
[cited by applicant]
WO WO2008145745A1
· 2008
[cited by applicant]
WO WO2008153801A1
· 2008
[cited by applicant]
WO WO2009045370A2
· 2009
[cited by applicant]
WO WO2009048560A1
· 2009
[cited by applicant]
WO WO2010042189A2
· 2010
[cited by applicant]
WO WO2011119773A1
· 2011
[cited by applicant]
WO WO2012122025A2
· 2012
[cited by applicant]
WO 2013053008A2
· 2013
[cited by applicant]
WO WO2013052799A2
· 2013
[cited by applicant]
WO WO2013052811A2
· 2013
[cited by applicant]
WO WO2013052832A2
· 2013
[cited by applicant]
WO 2013112549A1
· 2013
[cited by applicant]
WO WO2015095249A1
· 2015
[cited by applicant]
WO 2015106281A1
· 2015
[cited by applicant]
WO 2016024255A1
· 2016
[cited by applicant]
WO WO2016048903A1
· 2016
[cited by applicant]
WO WO2016145146A1
· 2016
[cited by applicant]
WO WO2017037280A1
· 2017
[cited by applicant]
WO WO2017062953A1
· 2017
[cited by applicant]
WO WO2017070284A1
· 2017
[cited by applicant]
WO WO2017083750A1
· 2017
[cited by applicant]
WO WO2017096432A1
· 2017
[cited by applicant]
WO WO2017132547A1
· 2017
[cited by examiner]
WO 2017210649A1
· 2017
[cited by applicant]
WO WO2019173463A1
· 2019
[cited by applicant]
WO WO2019173465A1
· 2019
[cited by applicant]
Khatun, Sabera, et al. “Safety and immunogenicity profile of human papillomavirus-16/18 AS04 adjuvant cervical cancer vaccine: a randomized controlled trial in healthy adolescent girls of Bangladesh.” Japanese journal o…
[cited by examiner]
NCBI NIH ANKRD49 gene listing, https://www.ncbi.nlm.nih.gov/gene/54851. (Year: 2024).
[cited by examiner]
Tsang, Kwong Y., et al. “Identification and characterization of enhancer agonist human cytotoxic T-cell epitopes of the human papillomavirus type 16 (HPV16) E6/E7.” Vaccine 35.19 (2017): 2605-2611. (Year: 2017).
[cited by examiner]
Paolini, Francesca, et al. “HPV 16 E5 oncoprotein is expressed in early stage carcinogenesis and can be a target of immunotherapy.” Human Vaccines & Immunotherapeutics 13.2 (2017): 291-297. (Year: 2017).
[cited by examiner]
Cordeiro et al, Human Vaccine & Immunotherapeutics (2015), 11:45-52.
[cited by applicant]
Martinez-Zapien et al, Nature (2016), 529:541-545.
[cited by applicant]
Altschul, S.F., et al., “Basic Local Alignment Search Tool,” Journal of Molecular Biology 215(3):403-410, Elsevier, United Kingdom (Oct. 1990).
[cited by applicant]
Altschul, S.F., et al., “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs,” Nucleic Acids Research 25(17):3389-3402, Oxford University Press, United Kingdom (Sep. 1997).
[cited by applicant]
Bai, M., et al., “Mutations That Alter an Arg-gly-asp (Rgd) Sequence in the Adenovirus Type 2 Penton Base Protein Abolish Its Cell-rounding Activity and Delay Virus Reproduction in Flat Cells,” Journal of Virology 67(9)…
[cited by applicant]
Biegert, A., and Soding, J., “Sequence Context-specific Profiles for Homology Searching,” Proc Natl Acad Sci USA 106(10):3770-3775, National Academy of Sciences, United States (Mar. 2009).
[cited by applicant]
Bird, R.E., et al., “Single-chain Antigen-binding Proteins,” Science 242(4877):423-426, Association for the Advancement of Science, United States (Oct. 1988).
[cited by applicant]
Boulanger, P., et al., “Characterization of Adenovirus Protein Ix,” The Journal of General Virology 44(3):783-800, Press for the Society for General Microbiology, United Kingdom (Sep. 1979).
[cited by applicant]
Brash, D.E., et al., “Strontium Phosphate Transfection of Human Cells in Primary Culture: Stable Expression of the Simian Virus 40 Large-T-Antigen Gene in Primary Human Bronchial Epithelial Cells,” Molecular and Cellula…
[cited by applicant]
Brough, D.E., et al., “Activation of Transgene Expression by Early Region 4 Is Responsible for a High Level of Persistent Transgene Expression From Adenovirus Vectors in Vivo,” Journal of Virology 71(12):9206-9213, Amer…
[cited by applicant]
Chen, H.H., et al., “Persistence in Muscle of an Adenoviral Vector that Lacks all Viral Genes,” Proc Natl Acad Sci USA 94(5):1645-1650, National Academy of Sciences, United States (Mar. 1997).
[cited by applicant]
Chen, Y.F., et al., “Cytotoxic-t-lymphocyte Human Papillomavirus Type 16 E5 Peptide With Cpg-oligodeoxynucleotide Can Eliminate Tumor Growth in C57b1/6 Mice,” Journal of Virology 78(3):1333-1343, American Society for Mi…
[cited by applicant]
Christopherson, K.S., et al., “Ecdysteroid-dependent Regulation of Genes in Mammalian Cells by a
[cited by applicant]
Chroboczek, J., et al., “The Sequence of the Genome of Adenovirus Type 5 and Its Comparison With the Genome of Adenovirus Type 2,” Virology 186(1):280-285, Academic Press, United States (Jan. 1992).
[cited by applicant]
Colbere-Garapin, F., et al., “A New Dominant Hybrid Selective Marker for Higher Eukaryotic Cells,” Journal of Molecular Biology 150(1):1-14, Academic Press, United Kingdom (Jul. 1981).
[cited by applicant]
Conese, M., et al., “Gene therapy progress and prospects: episomally maintained self-replicating systems,” Gene Therapy 11(24):1735-41, Nature Publishing Group, United Kingdom (2004).
[cited by applicant]
Corpet, F, “Multiple Sequence Alignment With Hierarchical Clustering,” Nucleic Acids Research 16(22):10881-10890, Oxford University Press, United Kingdom (Nov. 1988).
[cited by applicant]
Crawford-Miksza, L.C., and Schnurr, D.P., “Analysis of 15 Adenovirus Hexon Proteins Reveals the Location and Structure of Seven Hypervariable Regions Containing Serotype-specific Residues,” Journal of Virology 70(3): 18…
[cited by applicant]
Curiel, D.T., et al., “High-efficiency gene transfer mediated by adenovirus coupled to DNA-polylysine complexes,”
[cited by applicant]
Devaux, C., et al., “Structure of Adenovirus Fibre. I. Analysis of Crystals of Fibre From Adenovirus Serotypes 2 and 5 by Electron Microscopy and X-ray Crystallography,” Journal of Molecular Biology 215(4):567-588, Acad…
[cited by applicant]
Field, J., et al., “Properties of the Adenovirus DNA Polymerase,” The Journal of Biological Chemistry 259(15):9487-9495, American Society for Biochemistry and Molecular Biology, United States (Aug. 1984).
[cited by applicant]
Funston, G.M., et al., “Expression of Heterologous Genes in Oncolytic Adenoviruses Using Picornaviral 2a Sequences That Trigger Ribosome Skipping,” The Journal of General Virology 89(Pt 2):389-396, Cambridge Univ. Press…
[cited by applicant]
Gall, J.G.D., et al., “Construction and Characterization of Hexon-chimeric Adenoviruses: Specification of Adenovirus Serotype,” Journal of Virology 72(12):10260-10264, American Society for Microbiology, United States (S…
[cited by applicant]
Genbank, “E3 14.7K [Human adenovirus 5],” Accession No. AP_000224.1, accessed at https://www.ncbi.nlm.nih.gov/protein/AP_000224, accessed on Dec. 10, 2020, 1 page.
[cited by applicant]
GenBank, “E3 12.5K [Human adenovirus 5],” Accession No. AP_000218.1, accessed at https://www.ncbi.nlm.nih.gov/protein/56160551/, accessed on Dec. 10, 2020, 1 page.
[cited by applicant]
GenBank, “Hepatitis B virus, complete genome,” Accession No. Y07587.1, accessed at https://www.ncbi.nlm.nih.gov/nuccore/Y07587 on Dec. 15, 2020.
[cited by applicant]
Ghosh-Choudhury, G., et al., “Protein Ix, A Minor Component of the Human Adenovirus Capsid, Is Essential for the Packaging of Full Length Genomes,” The EMBO Journal 6(6):1733-1739, Wiley Blackwell, United Kingdom (Jun. …
[cited by applicant]
Ginsberg, H.S., et al., “A Proposed Terminology for the Adenovirus Antigens and Virion Morphological Subunits,” Virology 28(4):782-783, Academic Press, United States (Apr. 1966).
[cited by applicant]
Graham, F.L., et al., “Characteristics of a Human Cell Line Transformed by Dna From Human Adenovirus Type,” The Journal of General Virology 36(1):59-74, Microbiology Society, United Kingdom (Jul. 1977).
[cited by applicant]
Green, N.M., et al., “Evidence for a Repeating Cross Sheet Structure in the Adenovirus Fibre,” The EMBO Journal 2(8):1357-1365, IRL Press Limited, United Kingdom (Jun. 1983).
[cited by applicant]
Henikoff, S. and Henikoff, J.G., “Amino Acid Substitution Matrices from Protein Blocks,” Proc Natl Acad Sci USA 89(22):10915-10919, National Academy of Sciences, United States (Nov. 1992).
[cited by applicant]
Henry, L.J., et al., “Characterization of the Knob Domain of the Adenovirus Type 5 Fiber Protein Expressed in
[cited by applicant]
Higgins, D.G and Sharp, P.M, “Clustal: A Package for Performing Multiple Sequence Alignment on a Microcomputer,” Gene 73(1):237-244, Elsevier, Netherlands (Dec. 1988).
[cited by applicant]
Higgins, D.G., and Sharp, P.M., “Fast and Sensitive Multiple Sequence Alignments on a Microcomputer,” Computer Applications in the Biosciences 5(2):151-153, Oxford University Press, United Kingdom (Apr. 1989).
[cited by applicant]
Holliger, P, and Hudson, P.J., “Engineered Antibody Fragments and the Rise of Single Domains,” Nature Biotechnology 23(9):1126-1136, Nature Publishing Group, United Kingdom (2005).
[cited by applicant]
Horwitz, M.S., “Adenoviridae and their replication” in
[cited by applicant]
Huang, X., et al., “Parallelization of a Local Similarity Algorithm,” Computer Applications in the Biosciences 8(2):155-165, Oxford University Press, United Kingdom (Apr. 1992).
[cited by applicant]
Hurton, L.V., et al., “Tethered Il-15 Augments Antitumor Activity and Promotes a Stem-cell Memory Subset in Tumor-specific T Cells,” Proc Natl Acad Sci USA 113(48): E7788-E7791, National Academy of Sciences, United Stat…
[cited by applicant]
Huston, J.S., et al., “Protein Engineering of Antibody Binding Sites: Recovery of Specific Activity in an Anti-digoxin Single-chain Fv Analogue Produced in
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2019/020930, ISA/US, Alexandria, VA, mailed on Jul. 22, 2019, 12 pages.
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2019/020933, ISA/US, Alexandria, VA, mailed on Jul. 23, 2019, 13 pages.
[cited by applicant]
Ivics, Z., et al., “Molecular Reconstruction of Sleeping Beauty, a Tc1-like Transposon From Fish, and Its Transposition in Human Cells,” Cell 91(4):501-510, MIT Press, United States (Nov. 1997).
[cited by applicant]
Jin, Z., et al., “The Hyperactive Sleeping Beauty Transposase Sb100x Improves the Genetic Modification of T Cells to Express a Chimeric Antigen Receptor,” Gene Therapy 18(9):849-856, Macmillan Press Ltd, United Kingdom …
[cited by applicant]
Johnston, S.A., “Biolistic Transformation: Microbes to Mice,” Nature 346(6286):776-777, Nature Publishing Group, United Kingdom (Aug. 1990).
[cited by applicant]
Jornvall, H., et al., “The Adenovirus Hexon Protein. The Primary Structure of the Polypeptide and Its Correlation With the Hexon Gene,” The Journal of Biological Chemistry 256(12):6181-6186, American Society for Biochem…
[cited by applicant]
Kent, R.B., et al., “Ouabain resistance conferred by expression of the cDNA for a murine Na+, K+-ATPase alpha subunit,” Science 237(4817):901-903, American Association for the Advancement of Science, United States (Aug.…
[cited by applicant]
Kochanek, S., “High-capacity Adenoviral Vectors for Gene Transfer and Somatic Gene Therapy,” Human Gene Therapy 10(15):2451-2459, Mary Ann Liebert Inc., United States (Oct. 1999).
[cited by applicant]
Lowy, I., et al., “Isolation of Transforming DNA: Cloning the Hamster aprt Gene,” Cell 22(3):817-823, Cell Press, United States (Dec. 1980).
[cited by applicant]
Lutz, P., et al., “The Product of the Adenovirus Intermediate Gene Ix Is a Transcriptional Activator,” Journal of Virology 71(7): 5102-5109, American Society For Microbiology, United States (Jul. 1997).
[cited by applicant]
Martinez-Zapien, D.M., et al., “Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53,” Nature 529(7587):541-545, Nature Publishing Group, United Kingdom (Jan. 2016).
[cited by applicant]
Mates, L., et al., “Molecular Evolution of a Novel Hyperactive Sleeping Beauty Transposase Enables Robust Stable Gene Transfer in Vertebrates,” Nature Genetics 41(6):753-761, Nature Publishing Group, United Kingdom (Jun…
[cited by applicant]
Mattila, P.S.,et al., “The Actions of Cyclosporin A and FK506 Suggest a Novel Step in the Activation of T Lymphocytes,” EMBO J 9(13):4425-4433, Wiley Blackwell, United Kingdom (Dec. 1990).
[cited by applicant]
Mitra, R., et al., “Functional Characterization of Piggybat from the Bat
[cited by applicant]
Morsy, M.A., et al., “An adenoviral vector deleted for all viral coding sequences results in enhanced safety and extended expression of a leptin transgene,” Proc Natl Acad Sci USA 95(14):7866-71, National Academy of Sci…
[cited by applicant]
Mountford, P.S and Smith, A.G., “Internal Ribosome Entry Sites and Dicistronic Rnas In Mammalian Transgenesis,” Trends in Genetics 11(5): 179-184, Elsevier Trends Journals, United Kingdom (May 1995).
[cited by applicant]
Mulligan, R.C. and Berg, P., “Selection for Animal Cells that Express the
[cited by applicant]
Mumtaz, S., et al., “Design of Liposomes for Circumventing the Reticuloendothelial Cells,” Glycobiology 1(5):505-510, IRL Press at Oxford University Press, United Kingdom (Nov. 1991).
[cited by applicant]
Needleman S.B., et al., “A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins,” Journal of Molecular Biology 48(3):443-453, Academic Press Inc., United States (Mar. 1970).
[cited by applicant]
Neumann, R., et al., “Determination of the Nucleotide Sequence for The Penton-Base Gene of Human Adenovirus Type 5,” Gene 69(1): 153-157, Elsevier B.V., Netherlands (Sep. 1988).
[cited by applicant]
No., D., et al., “Ecdysone-inducible Gene Expression in Mammalian Cells and Transgenic Mice,” Proc Natl Acad Sci USA 93(8):3346-3351, National Academy of Sciences, United States (Apr. 1996).
[cited by applicant]
Novelli, A., and Boulanger, P.A., “Deletion analysis of functional domains in baculovirus-expressed adenovirus type 2 fiber,” Virology 185(1):365-76, Elsevier, Netherlands (1991).
[cited by applicant]
Nuclear Receptors Nomenclature Committee, “A Unified Nomenclature System for the Nuclear Receptor Superfamily,” Cell 97(2):161-163, Cell Press, United States (Apr. 1999).
[cited by applicant]
O'Hare, K., et al., “Transformation of Mouse Fibroblasts to Methotrexate Resistance by a Recombinant Plasmid Expressing a Prokaryotic Dihydrofolate Reductase,” Proc Natl Acad Sci USA 78(3):1527-1531, National Academy of…
[cited by applicant]
Osbourn, J.K., et al., “Directed Selection of Mip-1 Alpha Neutralizing Ccr5 Antibodies from a Phage Display Human Antibody Library,” Nature Biotechnology 16(8):778-781, Nature Publishing Group, United Kingdom (Aug. 1998…
[cited by applicant]
Paul, W. E., ed., “Fundamental Immunology,” 3rd Edition, pp. 353-363, Raven Press, United States (1993).
[cited by applicant]
Pearson, W.R. and Lipman, D.J., “Improved Tools for Biological Sequence Comparison,” Proc Natl Acad Sci USA 85(8):2444-2448, National Academy of Sciences, United States (Apr. 1988).
[cited by applicant]
Pearson, W.R., “Using the FASTA Program to Search Protein and DNA Sequence Databases,” Methods in Molecular Biology 24:307-331, Humana Press, United States (Feb. 1994).
[cited by applicant]
Roberts, M.M., et al., “Three-Dimensional Structure of the Adenovirus Major Coat Protein Hexon,” Science 232(4754):1148-1151, American Association for the Advancement of Science, United States (May 1986).
[cited by applicant]
Rux, J.J., et al., “Structural and Phylogenetic Analysis of Adenovirus Hexons by Use of High-Resolution X-Ray Crystallographic, Molecular Modeling, and Sequence-Based Methods,” Journal of Virology 77(17): 9553-9566, Ame…
[cited by applicant]
Santerre, R.F., et al., “Expression of Prokaryotic Genes for Hygromycin B and G418 Resistance as Dominant-selection Markers in Mouse L Cells,” Gene 30(1-3):147-156, Elsevier, Netherlands (Oct. 1984).
[cited by applicant]
Schirmbeck, R., et al., “Targeting Murine Immune Responses To Selected T Cell- or Antibody-Defined Determinants of the Hepatitis B Surface Antigen By Plasmid DNA Vaccines Encoding Chimeric Antigen,” Journal of Immunolog…
[cited by applicant]
Signas, C., et al., “Adenovirus 3 Fiber Polypeptide Gene: Implications for the Structure of the Fiber Protein,” Journal of Virology 53(2):672-678, American Society For Microbiology, United States (Feb. 1985).
[cited by applicant]
Smith, T.F., and Waterman, M.S., “Comparison of biosequences,”
[cited by applicant]
Soding, J., “Protein Homology Detection by Hmm-hmm Comparison,” Bioinformatics 21(7):951-960, Oxford University Press, United Kingdom (Apr. 2005).
[cited by applicant]
Soleimanjahi, H., et al., “Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine,” Iranian Journal of Immunology 14(3):180-191, Shiraz University of Medical Sciences, Iran (Sep. 2017).
[cited by applicant]
Stewart , P.L., et al., “Image Reconstruction Reveals the Complex Molecular Organization of Adenovirus,” Cell 67(1):145-154, Cell Press, United States (Oct. 1991).
[cited by applicant]
Stewart, P.L., et al., “Difference Imaging of Adenovirus: Bridging the Resolution Gap between X-Ray Crystallography and Electron Microscopy, ” EMBO Journal 12(7):2589-2599, Wiley Blackwell, United Kingdom (Jul. 1993).
[cited by applicant]
Suhr, S.T., et al., “High Level Transactivation by a Modified Bombyx Ecdysone Receptor in Mammalian Cells Without Exogenous Retinoid X Receptor,” Proc Natl Acad Sci USA 95(14):7999-8004, National Academy of Sciences, Un…
[cited by applicant]
Szybalska, E.H. and Szybalski, W., “Genetics of Human Cess Line IV DNA-mediated Heritable Transformation of A Biochemical Trait,” Proc Natl Acad Sci USA 48:2026-2034, National Academy of Sciences, United States (Dec. 19…
[cited by applicant]
Tsang, K.Y., et al., “Identification and characterization of enhancer agonist human cytotoxic T-cell epitopes of the human papillomavirus type 16 (HPV16) E6/E7,”
[cited by applicant]
Ui-Tei, K., et al., “Sensitive Assay of RNA Interference in
[cited by applicant]
Van Oostrum, J., et al., “Molecular Composition of the Adenovirus Type 2 Virion,” Journal of Virology 56(2):439-448, American Society for Microbiology, United States (Nov. 1985).
[cited by applicant]
Wieking, B.G., et al., “A non-oncogenic HPV 16 E6/E7 vaccine enhances treatment of HPV expressing tumors,” Cancer Gene Therapy 19(10):667-674, Nature Publishing Group, United Kingdom (Oct. 2012).
[cited by applicant]
Wigler, M., et al., “Transfer of Purified Herpes Virus Thymidine Kinase Gene to Cultured Mouse Cells,” Cell 11(1):223-232, Cell Press, United States (May 1977).
[cited by applicant]
Wigler, M., et al., “Transformation of Mammalian Cells with an Amplifiable Dominant-Acting Gene,” Proc Natl Acad Sci USA 77(6):3567-3570, National Academy of Sciences, United States (Jun. 1980).
[cited by applicant]
Wilson, M.H., et al., “Piggybac Transposon-Mediated Gene Transfer in Human Cells,” Molecular Therapy 15(1):139-145, Cell Press, United States (Jan. 2007).
[cited by applicant]
Yan, J., et al., “Induction of antitumor immunity in vivo following delivery of a novel HPV-16 DNA vaccine encoding an E6/E7 fusion antigen,” Vaccine 27(3):431-440, Elsevier Science, Netherlands (2009).
[cited by applicant]
Yeh, H.Y., et al., “Human adenovirus type 41 contains two fibers,” Virus Res 33(2):179-98, Elsevier, Netherlands (Aug. 1994).
[cited by applicant]
Co-pending Application, U.S. Appl. No. 16/978,570, inventors Brough, D.E., et al., international filing date: Mar. 6, 2019 (Not Published).
[cited by applicant]
Kaczkowski B. et al., “Integrative analyses reveal novel strategies in HPV11,-16 and -45 early infection”, Sci. Rep., 2:515, Jul. 17, 2012.
[cited by applicant]
Bin L. et al., “Ankyrin repeat domain 1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum”, J. Allergy Clin. Immunol., 141(6), pp. 2085-2093, Published online Jan. 3…
[cited by applicant]
Kaczkowski et al., Sci. Rep., (2012), 2:515.
[cited by applicant]
Bin et al., J. Allergy Clin. Immunol. (2018), 141:2085-2093.
[cited by applicant]
Duncan et al., Virology (2013), 444:119-123.
[cited by applicant]