Multi-diagnostic kit for mosquito-borne diseases and method for the diagnosis of the diseases
View Patent ↗A composition includes a first primer set consisting of the nucleotide sequences of SEQ ID Nos.1 to 6, and a second primer set consisting of the nucleotide sequences of SEQ ID Nos. 7 to 11. A multi-diagnostic kit for mosquito-borne diseases includes the composition. The multi-diagnostic kit enables rapid and accurate detection of dengue virus and malaria parasites by using specific primer sets. The multi-diagnostic kit is applicable in regions with high incidences of mosquito-borne disease pandemics including Africa and Latin America since it exhibits excellent thermal stability even in harsh environments.
1 . A composition comprising:
a first primer set consisting of the nucleotide sequences of SEQ ID Nos.1 to 6; and
a second primer set consisting of the nucleotide sequences of SEQ ID Nos. 7 to 11.
2 . The composition according to claim 1 , further comprising a third primer set consisting of the nucleotide sequences of SEQ ID Nos. 12 to 16.
3 . The composition according to claim 1 , wherein the composition further comprises hydroxy naphthol blue (HNB).
4 . The composition according to claim 1 , wherein the composition further comprises betaine.
5 . A multi-diagnostic kit for dengue and malaria, comprising the composition of claim 1 .
6 . The multi-diagnostic kit according to claim 5 , wherein the composition further comprises a third primer set consisting of the nucleotide sequences of SEQ ID Nos. 12 to 16.
7 . The multi-diagnostic kit according to claim 5 , wherein the composition further comprises hydroxy naphthol blue (HNB).
8 . The multi-diagnostic kit according to claim 5 , wherein the composition further comprises betaine.
9 . The multi-diagnostic kit according to claim 5 , wherein the composition comprises a first reagent composition comprising the first primer set and a second reagent composition comprising the second primer set.
10 . A method for diagnosing dengue and malaria, the method comprising:
adding DNA extracted from a sample to the composition according to claim 1 , wherein the sample is blood, saliva, urine, or tissue; and
performing a loop-mediated isothermal amplification of the DNA.
11 . The method according to claim 10 , wherein the amplification is carried out at 60 to 65° C. for 0.5 to 1 hour.
12 . The method according to claim 10 , wherein the composition further comprises a third primer set consisting of the nucleotide sequences of SEQ ID Nos. 12 to 16, and an infection status of Dengue virus, Plasmodium falciparum, and Plasmodium vivax is individually indicated.
13 . The method according to claim 10 , wherein the composition further comprises a third primer set consisting of the nucleotide sequences of SEQ ID Nos. 12 to 16.
14 . The method according to claim 10 , wherein the composition further comprises hydroxy naphthol blue (HNB).
15 . The method according to claim 10 , wherein the composition further comprises betaine.