IP Library Granted Patent US 12,507,697
Granted Patent B2
US 12,507,697 · App. 18/631,626 · Granted Dec 30, 2025

Population genetics approach to biological control of mycotoxin production

Inventor: Ignazio Carbone (Raleigh, NC)
Assignee: NORTH CAROLINA STATE UNIVERSITY
A01N63/34A01N63/30
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Quick Facts
Patent No.
US 12,507,697
App. No.
18/631,626
Granted
Dec 30, 2025
Kind
B2
Abstract

The invention relates to methods for controlling mycotoxin production in field populations of heterothallic and homothallic filamentous fungi through application of biocontrol compositions comprising selected strains of the heterothallic and homothallic filamentous fungi, the selected strains having a lineage of low mycotoxin production or no mycotoxin production and having opposite mating types (heterothallic) or both mating types (homothallic) (e.g., MAT1-1, MAT1-2).

Claims (18)

1 . A biocontrol method for reducing mycotoxin production in a field population of one or more heterothallic filamentous fungi, the method comprising:

applying a biocontrol composition to a plant, a plant part and/or a soil, the biocontrol composition comprising an effective amount of propagules of at least two strains of the one or more heterothallic filamentous fungi,

wherein the at least two strains comprise a first strain and a second strain of the one or more heterothallic filamentous fungi, each strain comprising a lineage of low mycotoxin production or no mycotoxin production,

wherein the first strain comprises a MAT1-1 mating type and the propagules are sclerotia and the second strain comprises a MAT1-2 mating type and the propagules are conidia, or wherein the first strain comprises a MAT1-1 mating type and the propagules are conidia and the second strain comprises a MAT1-2 mating type and the propagules are sclerotia, and

wherein the amount of propagules of the at least two strains of the one or more heterothallic filamentous fungi is effective to promote mating between compatible mating partners in the field population and the at least two strains, thereby increasing the ratio of strains having the lineage of no or low mycotoxin production in the field population of the one or more heterothallic filamentous fungi and reducing mycotoxin production by the field population of the one or more heterothallic filamentous fungi.

2 . The biocontrol method of claim 1 , wherein the at least two strains of the one or more heterothallic filamentous fungi comprises four strains, a first strain, a second strain, a third strain and a fourth strain, each strain comprising a lineage of low mycotoxin production or no mycotoxin production,

wherein the first and second strains are from the same species and the third and fourth strains are from the same species, and the first and third strains are each from the same genus/species or a different genus/species, and the second and fourth strains are each from the same genus/species or a different genus/species,

wherein the first and third strains comprise a MAT1-1 mating type and the propagules are sclerotia, and the second and fourth strains comprise a MAT1-2 mating type and the propagules are conidia, or wherein the first and third strains comprise a MAT1-1 mating type and the propagules are conidia, and the second and fourth strains comprise a MAT1-2 mating type and the propagules are sclerotia, and

wherein the amount of propagules of the first strain, second strain, third strain and fourth strain is effective to promote mating between compatible mating partners in the field population and the four strains, thereby increasing the ratio of strains having the lineage of no mycotoxin production or low mycotoxin production in the field population of the heterothallic filamentous fungi and reducing mycotoxin production by the field population of the heterothallic filamentous fungi.

3 . The biocontrol method of claim 1 , wherein the one or more heterothallic filamentous fungi is from the genus Fusarium , and/or the genus Aspergillus.

4 . The biocontrol method of claim 1 , wherein at least one of the at least two strains of the one or more heterothallic filamentous fungi is a native strain that is a lineage of low mycotoxin production or no mycotoxin production.

5 . The biocontrol method of claim 1 , wherein the mycotoxin is aflatoxin.

6 . The biocontrol method of claim 5 , wherein the aflatoxin production in the field population is reduced to about 1 ppb to about 30 ppb, as measured in a plant sample.

7 . The biocontrol method of claim 1 , wherein the effective amount of propagules of each strain in the biocontrol composition is about 1×10 4 cfu per ml to about 5×10 10 cfu per ml of the biocontrol composition.

8 . The biocontrol method of claim 1 , wherein the ratio of the low mycotoxin producing lineage to a high mycotoxin producing lineage in the field population of heterothallic filamentous fungi is increased as compared to a control.

9 . The biocontrol method of claim 1 , wherein the reduction in mycotoxin production is sustained over a period of time of at least about 2 years.

10 . The biocontrol method of claim 1 , wherein the plant or plant part is corn, peanut, cotton, wheat, triticale, barley, oat, rice, sorghum, coffee, grape, or a tree nut.

11 . The biocontrol method of claim 1 , wherein the low aflatoxin production is less than about 10 μg/mL aflatoxin.

Continuity (3)
Continuation 17050751
Provisional Application 62664421 · Apr 30, 2018
Related Publication 20240276988A1 · Aug 22, 2024
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