Patent
The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.
1 . A composition comprising Bacillus velezensis M173, wherein the Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434, wherein the composition further comprises an agriculturally or horticulturally acceptable diluent, filler, solvent, spontaneous promoter, carrier, emulsifier, dispersant, preservative, antifreezing agent, thickener, adjuvant, or any combination thereof, and wherein the Bacillus velezensis M173 is freeze-dried.
2 . The composition of claim 1 , wherein the composition further comprises one or more additional biological control agents, one or more chemical agents, or any combination thereof.
3 . The composition according to claim 2 , wherein the one or more additional biological control agents is a bacterium selected from the group consisting of Bacillus, Lactobacillus, Bifidobacterium, Propionibacterium, Streptococcus, Lactococcus, Pediococcus, Enterococcus, Staphylococcus , or any combination thereof; and
the bacterium of the Bacillus is selected from the group consisting of Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus coagulans , or any combination thereof.
4 . The composition according to claim 1 , wherein application of the composition improves the survival rate of a plant at seedling stage compared to a plant at seedling stage where the composition is not applied, through one or more features selected from: (1) improving survival rate of the plant when transplanted at seedling stage; (2) promoting plant growth; (3) increasing the yield of plant fruits; (4) promoting the growth of a plant organ; (5) improving the resistance of the plant to a stress environment; (6) improving the resistance of the plant to a pathogen; and (7) any combination of (1)-(6).
5 . The composition of claim 3 , wherein the one or more additional biological control agents is Bacillus pumilus M101, wherein the Bacillus pumilus M101 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61962.
6 . The composition of claim 5 , wherein the Bacillus velezensis M173 and Bacillus pumilus M101 are mixed to a concentration of 1:5 to 5:1 v/v.
7 . The composition of claim 5 , wherein the composition has an ability to form a biofilm.
8 . A method for preventing and/or treating a plant disease caused by a pathogen, or preventing and/or alleviating plant damage or necrosis caused by a non-pathogen condition, comprising: applying to a plant, plant tissue or plant organ the composition according to claim 1 , wherein the plant organ comprises root, stem, leaf, flower, fruit, and seed.
9 . A method for preventing and/or treating a plant disease caused by a pathogen, or preventing and/or alleviating plant damage or necrosis caused by a non-pathogen condition, comprising: applying to a plant, plant tissue or plant organ the composition according to claim 2 , wherein the plant organ comprises root, stem, leaf, flower, fruit, and seed.
10 . A method for preventing and/or treating a plant disease caused by a pathogen, or preventing and/or alleviating plant damage or necrosis caused by a non-pathogen condition, comprising: applying to a plant, plant tissue or plant organ the composition according to claim 3 , wherein the plant organ comprises root, stem, leaf, flower, fruit, and seed.
11 . The method according to claim 8 , wherein the applying comprises root soaking, foliar spraying, mist spraying, composting, seed soaking, coating, field flooding, drop irrigation of the plant or plant organ, smearing the plant or plant organ, or dripping the plant or plant organ.
12 . The method according to claim 8 , wherein the plant tissue comprises meristem, protective tissue, ground tissue, and conducting tissue.
13 . The method according to claim 8 , wherein the pathogen is selected from Colletotrichum capsici, Botrytis cinerea, Ralstonia solanacearum, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum, Athelia rolfsii, Streptomyces scabies, Sclerotium rolfsii, Sclerotinia sclerotiorum, Fusarium oxysporum sp. cucumebrium Owen, Gaeumannomyces critici, Fusarium graminearum, Valsa mali, Glomerella cingulata, Rhizoctonia solani, Pyricularia grisea, Alternaria solani, Exserohilum turcicum, Bipolaria maydis, Phytophthora capsici, Phytophthora nicotianae, Pseudomonas syringae, Xanthomonas campestris, Erwinia carotorora, Xanthomonas campestris, Erwinia carotovora, Botrytis -cirerea cinerea, Phytophthora infestans, Exserohilum turcicum, Bipolaria maydis, Fusarium oxysporum f. sp. niveum, Verticillium dahliae, Fusarium oxysporum f. sp. vasinfectum, Phytophthora capsici, Phytophthora nicotianae , or any combination thereof.
14 . A method for improving the resistance of a plant to a pathogen or stress condition, or promoting plant growth, comprising: applying to the plant, plant tissue or plant organ the composition according to claim 1 , wherein the plant organ comprises root, stem, leaf, flower, fruit, and seed.
15 . A method for improving the resistance of a plant to a pathogen or stress condition, or promoting plant growth, comprising: applying to the plant, plant tissue or plant organ the composition according to claim 2 , wherein the plant organ comprises root, stem, leaf, flower, fruit, and seed.
16 . A method for improving the resistance of a plant to a pathogen or stress condition, or promoting plant growth, comprising: applying to the plant, plant tissue or plant organ the composition according to claim 3 , wherein the plant organ comprises root, stem, leaf, flower, fruit, and seed.
17 . The method according to claim 14 , wherein the pathogen is selected from a pathogenic bacterium, pathogenic fungus or virus.
18 . The method according to claim 14 , wherein the pathogen is selected from the group consisting of Colletotrichum capsici, Botrytis cinerea, Ralstonia solanacearum, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum, Athelia rolfsii, Streptomyces scabies, Sclerotium rolfsii, Sclerotinia sclerotiorum, Fusarium oxysporum sp. cucumebrium owen, Gaeumannomyces critici, Fusarium graminearum, Valsa mali, Glomerella cingulata, Rhizoctonia solani, Pyricularia grisea, Alternaria solani, Exserohilum turcicum, Bipolaria maydis, Phytophthora capsici, Phytophthora nicotianae, Pseudomonas syringae, Xanthomonas campestris, Erwinia carotorora, Xanthomonas campestris, Erwinia carotovora, Botrytis -cirerea cinerea, Phytophthora infestans, Exserohilum turcicum, Bipolaria maydis, Fusarium oxysporum f. sp. niveum, Verticillium dahliae, Fusarium oxysporum f. sp. vasinfectum, Phytophthora capsici, Phytophthora nicotianae , and any combination thereof.
19 . The method according to claim 14 , wherein the stress condition is selected from the group consisting of temperature, drought, soil salt content, drug-induced damage, soil hardening, acidic soil, alkaline soil, insufficient soil fertility, and any combination thereof.
20 . The method according to claim 14 , wherein the plant disease caused by the pathogen is selected from the group consisting of bacterial wilt of plants, soft rot of plants, stem rot of plants, or gray mold of plants.