Compositions and methods for the detection of a chromosomal translocation in
Provided are compositions and methods for the detection of a chromosomal location in Brassica napus . This disclosure is generally related to the field of plant molecular biology, and in particular embodiments, to the field of detecting a chromosomal translocation within plants. In certain embodiments, the chromosomal translocation occurs between the N7 and N16 chromosomes of Brassica napus . In other embodiments, the chromosomal translocation is a homoeologous chromosomal reciprocal translocation. Accordingly, this disclosure provides compositions and methods for the identification, detection and utilization of such a chromosomal translocation.
1 . A method of breeding a canola plant comprising a N7/N16 homoeologous chromosomal reciprocal translocation comprising SEQ ID NO: 2, the method comprising:
(a) amplifying nucleic acid from a canola plant, tissue, or cell with a primer pair consisting of SEQ ID NO: 13 and SEQ ID NO: 15 or the complements thereof under very high stringency conditions to generate am amplicon,
(b) hybridizing a labeled probe comprising SEQ ID NO: 511 with the amplicon to detect the N7/N16 homoeologous chromosomal reciprocal translocation of SEQ ID NO:2,
(b) selecting the canola plant, tissue, or cell with the N7/N16 homoeologous chromosomal reciprocal translocation of SEQ ID NO:2; and
(c) breeding the canola plant, to obtain a progeny of the canola plant, tissue, or cell comprising the N7/N16 homoeologous chromosomal reciprocal translocation comprising SEQ ID NO:2.
2 . The method of claim 1 , the method further comprising:
(a) quantitating the amplicon produced by an amplification reaction.
3 . The method of claim 2 , wherein quantitating the results of the amplification reaction comprises producing a signature profile.
4 . The signature profile of claim 3 , wherein the signature profile is selected from the group consisting of a melting temperature curve signature profile and a fluorescence signature profile.
5 . The method of claim 4 , wherein the signature profile is produced from an intercalating DNA dye, a cyanine dye, or a fluorescent dye.
6 . The method of claim 1 , the method further comprising determining the presence or absence of the N7/N16 homoeologous chromosomal reciprocal translocation by determining the nucleotide sequence of said nucleic acid.
7 . The method of claim 1 , the method further comprising determining the presence or absence of the N7/N16 homoeologous chromosomal reciprocal translocation by determining the size of said nucleic acid.
8 . The method of claim 7 , wherein said determining the size comprises HPLC or electrophoresis.