Patent
Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.
1. A gene expression cassette comprising a promoter operably linked to a heterologous coding sequence, wherein the promoter comprises a polynucleotide that has at least 98% sequence identity to SEQ ID NO:1, and wherein the promoter drives expression of the heterologous coding sequence.
2. The gene expression cassette of claim 1 , wherein the polynucleotide comprises an intron.
3. The gene expression cassette of claim 1 , wherein the polynucleotide comprises a 5′-untranslated region.
4. A recombinant vector comprising the gene expression cassette of claim 1 .
5. A transgenic cell comprising the gene expression cassette of claim 1 .
6. The transgenic cell of claim 5 , wherein the promoter comprises SEQ ID NO:1.
7. The transgenic cell of claim 5 , wherein the polynucleotide comprises an intron.
8. The transgenic cell of claim 5 , wherein the polynucleotide comprises a 5′-untranslated region.
9. The transgenic cell of claim 5 , wherein the transgenic cell is a transgenic plant cell.
10. A transgenic plant comprising the transgenic cell of claim 9 .
11. The transgenic plant of claim 10 , wherein the plant is selected from the group consisting of an Arabidopsis plant, a tobacco plant, a soybean plant, a canola plant and a cotton plant.
12. A transgenic seed from the transgenic plant of claim 10 .
13. The transgenic cell of claim 5 , wherein the promoter consists of a polynucleotide sequence of nucleotides 1-820 of SEQ ID NO:1.
14. The gene expression cassette of claim 1 , wherein the promoter consists of a polynucleotide sequence of nucleotides 1-820 of SEQ ID NO:1.
15. The gene expression cassette of claim 1 , wherein the heterologous coding sequence is operably linked to a 3′-untranslated region.
16. A recombinant vector comprising the gene expression cassette of claim 15 .
17. A transgenic cell comprising the gene expression cassette of claim 15 .
18. The transgenic cell of claim 17 , wherein the transgenic cell is a transgenic plant cell.
19. A transgenic plant comprising the transgenic plant cell of claim 18 .
20. The transgenic plant of claim 19 , wherein the plant is selected from the group consisting of an Arabidopsis plant, a tobacco plant, a soybean plant, a canola plant and a cotton plant.
21. A transgenic seed from the transgenic plant of claim 19 .