Granted Patent
B2
US 12,708,554 · App. 17/924,091 · Granted Aug 18, 2026
Biomaterial compositions and methods of delivery
Inventors:
Kevin Eisenfrats (Charlottesville, VA); Gregory Grover (Houston, TX); Rucha Bhat (Manassas, VA); Tyler Chiartas (Charlottesville, VA); Nicholas Matsumoto (Charlottesville, VA)
Assignee:
Contraline, Inc.
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Abstract
Delivery systems, compositions, and methods for forming and delivering biomaterials from two components are described. Specifically, a composition includes a first component and a second component that are each formulated to be crosslinked with the other to form a hydrogel. The first component and the second component are formulated to have an initial storage modulus (initial G′) and an initial loss modulus (initial G″) when initially combined such that a ratio of the initial G″ to the initial G′ is between about 5 and about 100. The first component and the second component are formulated to have a gelation storage modulus (gelation G′) and a gelation loss modulus (gelation G″) at a gelation time after the first component and the second component are combined such that a ratio of the gelation G″ to the gelation G′ is less than about 1. The gelation time is less than about 120 seconds.
Claims (32)
1 . A composition, comprising:
a first component and a second component, the first component formulated to be crosslinked with the second component to form a hydrogel;
the first component and the second component being formulated to have an initial storage modulus (initial G′) and an initial loss modulus (initial G″) when the first component and the second component are initially combined, a ratio of the initial G″ to the initial G′ being between about 5 and about 100; and
the first component and the second component being formulated to have a gelation storage modulus (gelation G′) and a gelation loss modulus (gelation G″) at a gelation time after the first component and the second component are combined, a ratio of the gelation G″ to the gelation G′ being less than about 0.1, the gelation time being less than about 120 seconds.
2 . The composition of claim 1 , wherein the gelation time is less than about 60 seconds.
3 . The composition of claim 1 , wherein:
the first component comprises a multi-arm polyethylene glycol terminated with one or more thiol; and
the second component comprises a multi-arm polyethylene glycol terminated with one or more maleimide.
4 . The composition of claim 1 , wherein at least one of the first component or the second component is dissolved in a solvent and has a weight percentage within the solvent of between about 1% and 30%.
5 . The composition of claim 1 , wherein at least one of the first component or the second component is dissolved in a solvent having a pH of between about 4.5 and 8.
6 . The composition of claim 1 , wherein the gelation time is less than about 30 seconds.
7 . The composition of claim 1 , wherein the gelation time is between about 1 second and 60 seconds.
8 . The composition of claim 4 , wherein the weight percentage is between about 1% and 20%.
9 . The composition of claim 8 , wherein the weight percentage is about 20%.
10 . The composition of claim 5 , wherein the pH is between about 4.5 and 5.25.
11 . The composition of claim 1 , wherein the first component and the second component are capable of crosslinking and forming an implantable network by way of a bio-orthogonal reaction.
12 . The composition of claim 1 , wherein the first component and the second component are formulated such that the hydrogel is substantially formed before being conveyed out of a delivery member.
13 . The composition of claim 1 , wherein the first component and the second component are formulated such that a viscoelastic substance is conveyed out of a delivery member.
14 . A composition, comprising:
a first component and a second component, the first component formulated to be crosslinked with the second component to form a hydrogel;
the first component and the second component being formulated to have an initial storage modulus (initial G′) and an initial loss modulus (initial G″) when the first component and the second component are initially combined, a ratio of the initial G″ to the initial G′ being between about 5 and about 100; and
the first component and the second component being formulated to have a gelation storage modulus (gelation G′) and a gelation loss modulus (gelation G″) at a gelation time after the first component and the second component are combined, a ratio of the gelation G″ to the gelation G′ being less than about 0.2, the gelation time being between about 1 second and 60 seconds.
15 . The composition of claim 14 , wherein the ratio of the gelation G″ to the gelation G′ is about 0.1.
16 . The composition of claim 14 , wherein the gelation time is less than about 30 seconds.
17 . The composition of claim 14 , wherein at least one of the first component or the second component is dissolved in a solvent having a pH of between about 4.5 and 8.
18 . The composition of claim 14 , wherein at least one of the first component or the second component is dissolved in a solvent and has a weight percentage within the solvent of between about 1% and 20%.
19 . The composition of claim 14 , wherein:
the first component comprises a multi-arm polyethylene glycol terminated with one or more amine; and
the second component comprises a multi-arm polyethylene glycol terminated with one or more activated ester.
20 . The composition of claim 1 , wherein:
the first component comprises a multi-arm polyethylene glycol terminated with one or more amine; and
the second component comprises a multi-arm polyethylene glycol terminated with one or more activated ester.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 8, 2022
From: EISENFRATS, KEVIN; GROVER, GREGORY; BHAT, RUCHA; CHIARTAS, TYLER; MATSUMOTO, NICHOLAS
To: CONTRALINE, INC.
Reel/Frame 061699/0340 →
Continuity (3)
Provisional Application
63056124
· Jul 24, 2020
Provisional Application
63024628
· May 14, 2020
Related Publication
20230190515A1
· Jun 22, 2023
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