Inhibitors of HIV membrane fusion
View Patent ↗Inhibitors of HIV membrane fusion and a method of identifying drugs or agents which inhibit binding of the N-helix coiled-coil and the C helix of HIV gp41 envelope protein.
1. A D-peptide which is a soluble, trimeric peptide model of the HIV gp41 hydrophobic pocket, wherein the D-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise the sequence: LLXLTVWGXKXLQXRXX (SEQ ID NO: 42), wherein L, T, V, W, O, K, Q and R are amino acid residues represented by the single letter amino acid code and X is any D-amino acid residue.
2. The D-peptide of claim 1 wherein the sequence which comprises 17 amino acid residues is selected from the group consisting of: SEQ ID NO: 20; SEQ ID NO: 26; and SEQ ID NO: 27.
3. An L-peptide which is a soluble, trimeric model of the HIV gp41 hydrophobic pocket, wherein the L-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise the sequence: LLXLTVWGXKXLQXRXX (SEQ ID NO: 42) wherein L, T, V, W, G, K, Q and R are amino acid residues represented by the single letter amino acid code and X is any L-amino acid residue.
4. The L-peptide of claim 3 wherein the sequence which comprises 17 amino acid residues is selected from the group consisting of: SEQ ID NO: 20; SEQ ID NO: 26; and SEQ ID NO: 27.
5. A D-peptide which is a soluble, trimeric peptide model of the HIV gp41 hydrophobic pocket, wherein the D-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise SEQ ID NO: 20.
6. A D-peptide which is a soluble, trimeric peptide model of the HIV gp41 hydrophobic pocket, wherein the D-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise SEQ ID NO: 26.
7. A D-peptide which is a soluble, trimeric peptide model of the HIV gp41hydrophobic pocket, wherein the D-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise SEQ ID NO: 27.
8. An L-peptide which is a soluble, trimeric model of the HIV gp41 hydrophobic pocket, wherein the L-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise SEQ ID NO: 20.
9. An L-peptide which is a soluble, trimeric model of the HIV gp41 hydrophobic pocket, wherein the L-peptide comprises SEQ ID NO: 25 and a sequenc which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise SEQ ID NO: 26.
10. An L-peptide which is a soluble, trimeric model of the HIV gp4 hydrophobic pocket, wherein the L-peptide comprises SEQ ID NO: 25 and a sequence which comprises 17 amino acid residues, wherein the 17 amino acid residues comprise SEQ ID NO: 27.