Method of stimulating growth of melanocyte cells by administering stem cell factor
Novel stem cell factors, oligonucleotides encoding the same, and methods of production, are disclosed. Pharmaceutical compositions and methods of treating disorders involving blood cells are also disclosed.
1. A method of stimulating proliferation of melanocyte cells in a human, the method comprising the step of administering to the human, a therapeutically effective amount of stem cell factor (SCF) polypeptide and optionally a pharmaceutically acceptable carrier.
2. The method of claim 1 wherein stem cell factor polypeptide selected is selected from the group consisting of amino acids 1-162, 1-164, and 1-165 as set out in SEQ ID NO: 46, said polypeptide optionally consisting of an N-terminal methionine.
3. The method of claim 1 wherein the stem cell factor polypeptide is selected from the group consisting of amino acids 1-130, 1-133, 1-137, 1-141, 1-145, 1-148, 1-152, 1-156, 1-157, 1-158, 1-159, 1-160, 1-161, 1-163, 1-166, 1-168, 1-173, 1-178, 2-164, 2-165, 5-164, 11-164, 1-180, 1-183, 1-185, 1-188, 1-189, 1-220, and 1-248 as set out in SEQ ID NO: 61, said polypeptide optionally consisting of an N-terminal methionine.
4. The method of claim 1 wherein the stem cell factor polypeptide is selected from the group consisting of amino acids 1-152, 1-157, 1-160, 1-161, and 1-220 as set out in SEQ ID NO: 63, said polypeptide optionally consisting of an N-terminal methionine.
5. The method of claim 1 wherein the stem cell factor is covalently conjugated to a water soluble polymer.
6. The method of claim 5 wherein the water soluble polymer is polyethylene glycol.
7. The method of claim 1 wherein the stem cell factor is co administered with at least one other cytokine.
8. The method of claim 5 wherein the stem cell factor is co administered with at least one other cytokine.
9. The method of claim 7 wherein one or more cytokines are selected from a group consisting of Interleukin-1 (IL-1), Interleukin-2 (IL-2), Interleukin-3 (IL-3), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-6 (IL-6), Interleukin-7 (IL-7), Interleukin-8 (IL-8), Interleukin-9 (IL-9), Interleukin-10 (IL-10), Interleukin-11 (IL-11), Interleukin-12 (IL-12), erythropoietin (EPO), Granulocyte Colony-stimulating Growth Factor (G-CSF), Macrophage Colony-Stimulating Factor (M-CSF), Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Insulin-like Growth Factor-1 (IGF-1), and Leukemic Inhibitory Factor (LIF).
10. The method of claim 8 wherein one or more cytokines are selected from a group consisting of IL-1, IL-2, IL-3, IL-4, IL5, IL-6, IL-7, IL 8, IL-9, IL-10, IL-11, IL-12, EPO, G-CSF, M-CSF, GM-CSF, IGF-1, and LIF.
11. The method of claim 1 wherein the pharmaceutically acceptable carrier is suitable for topical delivery.
12. The method of claim 1 wherein the pharmaceutically acceptable carrier is suitable for oral delivery.
13. The method of claim 1 wherein the pharmaceutically acceptable carrier is suitable for parenteral delivery.
14. The method of claim 1 wherein the pharmaceutically acceptable carrier is suitable for pulmonary delivery.
15. The method of claim 1 wherein the pharmaceutically acceptable carrier is suitable for nasal delivery.
16. A method of stimulating proliferation of melanocyte cells in a human, the method comprising the step of administering to the human, a therapeutically effective amount of a stem cell factor (SCF) polypeptide having the amino acid sequence of SEQ ID NO: 44, SEQ ID NO: 46, or SEQ ID NO: 63, or biologically active fragments thereof that stimulate growth of melanocyte cells, and optionally a pharmaceutically acceptable carrier.
17. The method of claim 16 wherein the stem cell factor is covalently conjugated to a water soluble polymer.
18. The method of claim 17 wherein the water soluble polymer is polyethylene glycol.
19. The method of claim 16 wherein the stem cell factor is co administered with at least one other cytokine.
20. The method of claim 17 wherein the stem cell factor is co administered with at least one other cytokine.
21. The method of claim 19 wherein one or more cytokines are selected from a group consisting of Interleukin-1 (IL-1), Interleukin-2 (IL-2), Interleukin-3 (IL-3), Interleukin-4 (IL4), Interleukin-5 (IL-5), Interleukin-6 (IL-6), Interleukin-7 (IL-7), Interleukin-8 (IL-8), Interleukin-9 (IL-9), Interleukin-10 (IL-10), Interleukin-11 (IL-11), Interleukin-12 (IL-12), erythropoietin (EPO), Granulocyte Colony-stimulating Growth Factor (G-CSF), Macrophage Colony-Stimulating Factor (M-CSF), Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Insulin-like Growth Factor-1 (IGF-1), and Leukemic Inhibitory Factor (LIF).
22. The method of claim 20 wherein one or more cytokines are selected from a group consisting of IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, EPO, G-CSF, M-CSF, GM-CSF, IGF-1, and LIF.
23. The method of claim 16 wherein the pharmaceutically acceptable carrier is suitable for topical delivery.
24. The method of claim 16 wherein the pharmaceutically acceptable carrier is suitable for oral delivery.
25. The method of claim 16 wherein the pharmaceutically acceptable carrier is suitable for parenteral delivery.
26. The method of claim 16 wherein the pharmaceutically acceptable carrier is suitable for pulmonary delivery.
27. The method of claim 16 wherein the pharmaceutically acceptable carrier is suitable for nasal delivery.