Granted Patent
B2
US 6,906,194 · App. 10/681,427 · Granted Jun 14, 2005
Fluorescence assay for kinase activity
Assignee:
Massachusetts Instititue of Technology
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Abstract
The present invention provides a sensor and methods for determining kinase activity.
Claims (12)
1. A compound of the formula (I):
where at least one R group is —SO 2 X, where X is —OR″ or —NR″R″′;
R′ is hydroxy, amino, or thiol;
R″ is C 1-6 alkyl;
R″′ is hydrogen or alkyl; and
n is 1, 2 or 3; and
wherein compound (I) undergoes chelation-enhanced fluorescence (CHEF) upon binding to Mg 2+ .
2. The compound of claim 1 , which is of the formula (II):
where X is —OR″ or —NR″R″′;
R″ is C 1-6 alkyl; and
R″′ is hydrogen or C 1-6 alkyl.
3. The compound of claim 1 , which is of the formula (III):
Assignments (4)
CONFIRMATORY LICENSE
Recorded May 12, 2011
From: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 026268/0984 →
CONFIRMATORY LICENSE
Recorded Sep 8, 2008
From: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 021495/0138 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 20, 2004
From: DAY, RICHARD A.; RENTZ, CHARLES THOMAS; HUANG, GREGORY SHANON; PATEL, AMIT B.; IQBAL, AMJAD M.; GRETZ, PAUL M.; SHEN, XIANGZHONG; CAO, XUEFEI
To: CINCINNATI, UNIVERSITY OF
Reel/Frame 015694/0142 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 8, 2003
From: IMPERIALI, BARBARA; SHULTS, MELISSA D.
To: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Reel/Frame 014605/0438 →
Continuity (1)