Method and apparatus for isolating a component from a fluid mixture

1. A method for isolating a proportion of a fluid mixture from the fluid mixture as a whole, said method comprising:

allowing the fluid mixture to flow through a measuring chamber;

applying an electrical signal at one or more frequencies to the proportion of the fluid mixture in the measuring chamber;

measuring an impedance quantity characteristics of the proportion of the fluid mixture in the measuring chamber at the one or more frequencies; and

causing the proportion of the fluid mixture to flow to a unique destination remote from the remainder of the fluid mixture when the impedance quantity measured at the one or more frequencies attains a target impedance quantity.

2. A method as claimed in claim 1 wherein the target impedance quantity is a calibrated impedance quantity.

3. A method as claimed in claim 1 wherein the measuring chamber is a microchamber.

4. A method as claimed in claim 1 wherein the proportion of the fluid mixture is a discrete component selected from the group consisting of cells and particles.

5. A method as claimed in claim 1 wherein the one or more frequencies at which the impedance quantity is measured includes a resonant frequency.

6. A method as claimed in claim 1 wherein the impedance quantity is the dissipation factor.

7. A method as claimed in claim 1 wherein the electrical signal is applied at each of a plurality of frequencies in a frequency range and the impedance quantity is measured at each of the plurality of frequencies in the frequency range.

8. A method as claimed in claim 7 wherein the plurality of frequencies includes a resonant frequency.

9. A method as claimed in claim 8 wherein the plurality of frequencies in the range are sufficient in number to generate an impedance spectrum characteristic of the proportion of the fluid mixture in the measuring chamber.

10. A method as claimed in claim 1 wherein the electrical signal is applied at a single frequency and the impedance quantity is measured at that frequency.

11. A method as claimed in claim 10 wherein the single frequency is at or near to the resonant frequency of the measuring chamber containing the proportion of the fluid mixture.

12. A method as claimed in claim 4 wherein the volume of the proportion of fluid mixture in the measuring chamber is less than 10 −6 liters.

13. A method as claimed in claim 12 wherein the fluid mixture is a binary system.

14. A method as claimed in claim 12 wherein the fluid mixture comprises two or more liquids.

15. A method as claimed in claim 13 wherein the fluid mixture comprises a liquid (or mixture of liquids) with one or more dissolved or suspended components.

16. A method as claimed in claim 15 wherein the fluid mixture comprises particles or cells suspended in a liquid medium.

17. A method as claimed in claim 16 wherein the fluid mixture is a blood extract consisting essentially of blood cells and liquid medium.

18. A method as claimed in claim 12 wherein the fluid mixture is a solution.

19. A method as claimed in claim 18 wherein the solution is a polymer containing solution.

20. A method as claimed in claim 2 further comprising the initial steps of:

allowing a calibrant fluid mixture to flow through a measuring chamber,

applying an electrical signal at one or more frequencies to the proportion of the calibrant fluid mixture in the measuring chamber;

measuring the calibrated impedance quantity of the proportion of the calibrant fluid mixture in the measuring chamber at the one or more frequencies; and

correlating the calibrated impedance quantity of the proportion of the calibrant fluid mixture in the measuring chamber with a characteristic of the proportion of the calibrant fluid mixture in the measuring chamber, wherein the characteristic of the proportion of the calibrant fluid mixture in the measuring chamber is known.

21. A method as claimed in claim 20 further comprising:

comparing the impedance quantity of the proportion of the fluid mixture measured in the measuring chamber with the calibrated impedance quantity.

22. A method as claimed in claim 21 comprising:

causing the proportion of the fluid mixture to flow to a unique destination when the impedance quantity measured at the one or more frequencies is substantially the same as the target impedance quantity at the one or more frequencies.

23. A method as claimed in claim 21 comprising:

causing the proportion of the fluid mixture to flow to a unique destination when the impedance quantity measured at the one or more frequencies is above the target impedance quantity.

24. A method as claimed in claim 21 further comprising:

sending an appropriate signal to a separating means in or adjacent to the flow path when the target impedance quantity is attained.

25. A method as claimed in claim 20 wherein the electrical signal is a time varying electrical signal.

26. An apparatus for isolating a proportion of a fluid mixture from the fluid mixture as a whole, said apparatus comprising:

an electrical impedance measuring device adapted to permit the fluid mixture to flow through a measuring chamber, wherein said device is capable of measuring an impedance quantity characteristic of the proportion of the fluid mixture in the measuring chamber;

separating means in or adjacent to the flow path of the fluid mixture at or near to the exit end of the measuring chamber; and

means for sending a signal to the separating means when the impedance quantity attains a target impedance quantity whereby to cause the proportion of the fluid mixture to flow to a unique destination remote from the remainder of the fluid mixture.

27. An apparatus as claimed in claim 26 wherein the separating means is a deflecting means in the flow path.

28. An apparatus as claimed in claim 26 wherein the electrical impedance measuring device comprises:

an electrical signal applying means adapted to apply a time varying electrical signal to the measuring chamber at one or more frequencies in a frequency range; and

measuring means for measuring an impedance quantity characteristic of the proportion of the fluid mixture in a measuring chamber at the one or more frequencies in the frequency range.

29. An apparatus as claimed in claim 28 wherein the electrical signal applying means comprises at least two microelectrodes.

30. An apparatus as claimed in claim 26 wherein the measuring chamber is configured so that measurement of an impedance quantity is conducted in an effective 1-dimensional electric field.

31. An apparatus as claimed in claim 30 wherein the measuring chamber is a microchamber or microchannel.