Apparatus and method for imaging
Apparatus for acquiring an image of a specimen comprising a cassette having an optical portion holding a specimen array on a TIR surface and being removably matable to a processing portion having a polarized light beam source and a processing polarization-sensitive portion to image the spatially distributed charges in polarization of the specimen array. In one form the array optical portion comprises a transparent slide having a bottom surface with first and second gratings located to direct polarized light to the TIR surface and to direct light reflected by that (TIR) surface to an imager, respectively. The apparatus may include a flow cell integral with the optical portion as well as means for selecting the direction and wavelength of the polarized light.
1. An optical assembly for use with an imaging apparatus, the optical assembly comprising:
an optical element including a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a light beam from a light source to the reflective surface, such that a reflection of the light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a locating portion coupled to the optical element, the locating portion engageable with a mating locating element of a detector adapted for providing an image based on spatially distributed polarization changes in the light beam.
2. The optical assembly of claim 1 , wherein the optical element includes a prism.
3. The optical assembly of claim 2 , wherein the optical element includes a transparent slide operably coupled to the prism.
4. The optical assembly of claim 1 , wherein the optical assembly and the light source are configured for a single reflection of the light beam at the reflective surface.
5. The optical assembly of claim 1 , wherein the reflective surface comprises the upper surface of a transparent slide, the slide having a lower surface, the lower surface including at least one grating and the upper surface including an array of receptors thereon.
6. The optical assembly of claim 5 , wherein the at least one grating comprises a first grating positioned to intercept the light beam from the light source and a second grating positioned to intercept the light beam after the light beam has reflected from the upper surface.
7. The optical assembly of claim 5 , wherein the at least one grating is selected from the group consisting of an electrically alterable grating, a Bragg grating, and an electrically alterable hologram.
8. The optical assembly of claim 1 , further comprising a flow cell coupled to the reflective surface, the flow cell including an inlet port and an outlet port for flowing analyte across the specimen array.
9. The optical assembly of claim 1 , wherein the locating portion includes a mount or a frame.
10. The optical assembly of claim 9 , wherein the mount or the frame is operable to locate at least a selected portion of the specimen array in the evanescent field.
11. A cassette for use with an imaging apparatus, the cassette comprising:
an optical element including a prism and a slide having a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a light beam from a light source to the reflective surface, such that a reflection of the light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a locating portion coupled to the optical element, the locating portion engageable with a mating locating element of a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam.
12. The cassette of claim 11 , wherein the cassette and the light source are configured for a single reflection of the light beam at the reflective surface.
13. The cassette of claim 11 , wherein the locating portion includes a mount or a frame.
14. The cassette of claim 13 , wherein the mount or frame is operable to locate at least a selected portion of the specimen array in the evanescent field.
15. An optical assembly for use with an imaging apparatus, the optical assembly comprising:
an optical element comprising a reflective surface to allow formation thereon of a specimen array, to optical element being configured to direct a light beam from a light source to the reflective surface, such that a reflection of the light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and
a locating portion coupled to the optical element, the locating portion engageable with a mating locating element of a processing assembly.
16. The optical assembly of claim 15 , wherein the processing assembly includes a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam.
17. The optical assembly of claim 15 , wherein the processing assembly includes the light source.
18. The optical assembly of claim 15 , wherein the reflective surface comprises the upper surface of a slide, the slide having a lower surface, the lower surface including at least one grating and the upper surface including an array of receptors thereon.
19. The optical assembly of claim 18 , wherein the at least one grating comprises a first grating positioned to intercept the light beam from the light source and a second grating positioned to intercept the light beam after the light beam has reflected from the upper surface.
20. The optical assembly of claim 18 , wherein the at least one grating is selected from the group consisting of an electrically alterable grating, a Bragg grating, and an electrically alterable hologram.
21. The optical assembly of claim 15 , further comprising a flow cell coupled to the reflective surface, the flow cell including an inlet port and an outlet port for flowing analyte across the specimen array.
22. The optical assembly of claim 15 , wherein the locating portion includes a mount or a frame.