Peptide-based immunotherapeutic agent
A peptide-based immunotherapeutic agent effective for every allergy patient is provided. A reagent for typing HLA class II molecules of the patient to be used in selecting a peptide-based immunotherapeutic agent effective for every allergy patient is also provided. The peptide-based immunotherapeutic agent enables the optimal peptide-based immunotherapy for each patient, so that a marked improvement in peptide-immunotherapy can be expected. The peptide-based immunotherapeutic agent is also effective for patients who cannot be treated by peptide-based immunotherapy using major antigen peptide recognized in a particular patient population. Furthermore, the peptide-based immunotherapeutic agent enables simple and easy typing of HLA class II molecules of allergy patients.
1. A method for treating Japanese cedar pollen allergy in a subject in need thereof, the method comprising:
(a) identifying an HLA class II molecule expressed by the subject;
(b) selecting an antigenic peptide derived from Japanese cedar pollen allergen Cry j 1 or Cry j 2, wherein the antigenic peptide binds to HLA class II molecule and wherein:
(1) when the HLA class II molecule identified in step (a) is DQA1*0102-DQB1*0602, the antigenic peptide is selected from the group consisting of SEQ ID NO: 1, 5, 7, 9, 10, 21, 23, and 142;
(2) when the HLA class II molecule identified in step (a) is DPA1*0101-DPB1*0501, the antigenic peptide is selected from the group consisting of SEQ ID NO: 2, 8, and 15;
(3) when the HLA class II molecule identified in step (a) is DPA1*0101-DPB1*0201, the antigenic peptide is SEQ ID NO: 17;
(4) when the HLA class II molecule identified in step (a) is DPA1*0202-DPB1*0501, the antigenic peptide is SEQ ID NO: 22;
(5) when the HLA class II molecule identified in step (a) is DRB5*0101, the antigenic peptide is selected from the group consisting of SEQ ID NO: 3, 4, 14, and 19;
(6) when the HLA class II molecule identified in step (a) is DRB1*0901, the antigenic peptide is selected from the group consisting of SEQ ID NO: 6, 7, 12, 16, and 20;
(7) when the HLA class II molecule identified in step (a) is DRB4*0101, the antigenic peptide is selected from the group consisting of SEQ ID NO: 7, 12, and 18; and
(8) when the HLA class II molecule identified in step (a) is DRB1*0501, the antigenic peptide is selected from the group consisting of SEQ ID NO: 13 and 19; and
(c) administering the selected antigenic peptide to the subject.
2. A customized pharmaceutical composition for treating a subject suffering from a Japanese cedar pollen allergy, the composition comprising:
(a) an effective amount of an antigenic peptide derived from Japanese cedar pollen allergen Cry j 1 or Cry j 2, wherein the antigenic peptide binds to an HLA class II molecule expressed by the subject and wherein:
(1) when the subject expresses the HLA class II molecule DQA1*0102-DQB1*0602, the antigenic peptide is selected from the group consisting of SEQ ID NO: 1, 5, 7, 9, 10, 21, 23, and 142;
(2) when the subject expresses the HLA class II molecule DPA1*0101-DPB1*0501, the antigenic peptide is selected from the group consisting of SEQ ID NO: 2, 8, and 15;
(3) when the subject expresses the HLA class II molecule DPA1*0101-DPB1*0201, the antigenic peptide is SEQ ID NO: 17;
(4) when the subject expresses the HLA class II molecule DPA1*0202-DPB1*0501, the antigenic peptide is SEQ ID NO: 22;
(5) when the subject expresses the HLA class II molecule DRB5*0101, the antigenic peptide is selected from the group consisting of SEQ ID NO: 3, 4, 14, and 19;
(6) when subject expresses the HLA class II molecule DRB1*0901, the antigenic peptide is selected from the group consisting of SEQ ID NO: 6, 7, 12, 16, and 20;
(7) when the subject expresses the HLA class II molecule DRB4*0101, the antigenic peptide is selected from the group consisting of SEQ ID NO: 7, 12, and 18; and
(8) when the subject expresses the HLA class II molecule DRB1*0501, the antigenic peptide is selected from the group consisting of SEQ ID NO: 13 and 19; and
(b) a pharmaceutically acceptable diluent or carrier.