Methods for screening for compounds that modulate pd-1 signaling
View Patent ↗Disclosed are screening assays for identifying compounds which modulate the activity of, or signaling via, B7-4, or PD-1, especially which modulate the binding of B7-4 or PD-1 to a target molecule Further disclosed are a vaccine and also other compositions which contain agents that modulate signaling via PD-1.
1. A method for screening for compounds which modulate PD-1 signaling comprising:
a) contacting a T cell activated in the presence of PD-L1 with a test compound;
b) contacting a T cell activated in the absence of PD-L1 with the test compound; and
c) determining the ability of the test compound to modulate phosphorylation of ERK 1 or ERK 2;
wherein modulation of phosphorylation of ERK 1 or ERK 2 in the presence of PD-L1 and not in the absence of PD-L1 indicates modulation of PD-1 signaling, thereby identifying a compound which modulates PD-1 signaling.
2. The method of claim 1 wherein the test compound upmodulates PD-1 signaling and thereby downmodulates of ERK 1 or ERK 2.
3. The method of claim 1 wherein the test compound downmodulates PD-1 signaling and thereby upmodulates of ERK 1 or ERK 2.
4. A method for screening for compounds which modulate PD-1 signaling comprising:
a) contacting a T cell activated in the presence of PD-L1 with a test compound;
b) contacting a T cell activated in the absence of PD-L1 with the test compound; and
c) determining the ability of the test compound to modulate phosphorylation of PKC-θ;
wherein modulation of phosphorylation of PKC-θ in the presence of PD-L1 and not in the absence of PD-L1 indicates modulation of PD-1 signaling, thereby identifying a compound which modulates PD-1 signaling.
5. The method of claim 4 wherein the test compound upmodulates PD-1 signaling and thereby downmodulates phosphorylation of PKC-θ.
6. The method of claim 4 wherein the test compound downmodulates PD-1 signaling and thereby upmodulates phosphorylation of PKC-θ.