Culturable mitochondrial cells with no nucleus, showing mitochondrial activity
Provided are a method of producing a culturable cell with no nucleus, showing mitochondrial activity, comprising: performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cell derived from a cancer cell; culturing resulting cybrid cells; and recovering floating cells from obtained cultured cells, and a cell obtained by the method.
1. A method of producing a culturable cell population, showing mitochondrial activity, wherein the cell population contains at least one cell with a nucleus and cells with no nucleus, and wherein 95% or more among the cell population are cells with no nucleus, comprising:
performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cancer cell or cell of an established cell line;
culturing resulting cybrid cells; and
recovering floating cells which separate from adherent cybrid cells during the culture thereof.
2. A method according to claim 1 , wherein the nucleus-less cell having mitochondrial DNA is a platelet.
3. A method according to claim 1 , wherein the mitochondrial DNA-less cultured cell is a Hela Rho 0 cell.
4. A culturable cell population, showing mitochondrial activity obtained by the method according to any one of claims 1 to 3 .
5. A method of producing a population of cells with no nucleus showing mitochondrial activity, comprising:
performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cancer cell or cell of an established cell line;
culturing resulting cybrid cells;
recovering floating cells which separate from adherent cybrid cells during the culture thereof;
determining the presence or absence of a nucleus in the floating cells; and
selecting cells with no nucleus.