Polynucleotide and polypeptide fat metabolism regulators and uses thereof
In general, this invention relates to nucleic acid and amino acid sequences involved in fat metabolism regulation and the use of these sequences as targets for the diagnosis, treatment, and prevention of obesity and obesity-related diseases. In addition, the invention relates to screening methods for identifying modulators of body fat metabolism and the development of treatments for obesity and obesity-related diseases.
1. A method of identifying a candidate compound that modulates fat metabolism, said method comprising
a) contacting a mammalian cell or a nematode cell with a candidate compound;
b) obtaining mRNA from said mammalian cell or said nematode cell;
c) contacting a microarray with said mRNA, wherein said microarray consists of at least two mammalian or nematode fat metabolism regulator nucleic acids or fragment thereof, wherein said fragment encodes at least 50 contiguous amino acids of a fat metabolism polypeptide, and wherein inactivation of each of said mammalian or nematode fat metabolism regulator nucleic acids results in an alteration in fat content of an organism compared to a control organism; and
d) detecting an alteration in cellular mRNA levels of at least two fat metabolism regulator nucleic acid molecules in said mammalian cell or said nematode cell contacted with said candidate compound compared to a control cell, wherein said alteration identifies the candidate compound as a candidate compound that modulates fat metabolism.
2. The microarray of claim 1 , wherein said mammalian cell is contacted with said candidate compound and said microarray consists of at least two mammalian fat metabolism regulator nucleic acids.
3. The microarray of claim 1 , wherein said nematode cell is contacted with said candidate compound and said microarray consists of at least two nematode fat metabolism regulator nucleic acids.