Cartilage intermediate layer protein
A novel, N-oligosaccharide-substituted-specific protein of approximately 91.5 kDa was purified from extracts of human articular cartilage and designated Cartilage Intermediate Layer Protein (CILP). The protein is a chondrocyte product which is deposited in the interterritorial matrix. The gene encoding CILP, as well as its mRNA, were isolated and characterized. A single 4.2 kb mRNA detected in human articular cartilage encodes a polypeptide of 1184 amino acids with a calculated molecular weight of 132.5 kDa. The protein has a putative signal peptide of 21 amino acids, and is a proform of two polypeptides. The amino-terminal half corresponds to CILP (molecular weight of 78.5 kDa, not including post-translational modifications) and the carboxy-terminal half corresponds to a protein homologous to a porcine nucleotide pyrophosphohydrolase, NTPPHase (molecular weight of 51.8 kDa, not including post-translational modifications).
1. An isolated or purified cartilage intermediate layer protein (CILP), wherein said CILP consists of residues 1–703 of SEQ ID NO:2 and has a molecular mass of 92,000 when expressed in a cell.
2. An isolated or purified cartilage intermediate layer protein (CILP), wherein said CILP is produced by expressing a nucleic acid comprising the sequence of SEQ ID NO:1 in a host cell and permitting the host cell to cleave the polypeptide encoded by said nucleic acid into said CILP and a nucleotide pyrophosphohydrolase, wherein said CILP has a molecular mass of 92,000 after processing by the cell.
3. An isolated or purified cartilage intermediate layer protein (CILP) encoded by SEQ ID NO:1.
4. An isolated or purified peptide consisting of residues 1–703 of SEQ ID NO:2.
5. The peptide of claim 4 , wherein the peptide is a mammalian peptide.
6. The peptide of claim 4 , wherein the peptide is a human peptide.
7. The peptide of claim 4 , wherein the peptide is a recombinant peptide.
8. The peptide of claim 4 , wherein the peptide is isolated from chondrocyte-containing tissues.
9. The peptide of claim 4 , wherein the peptide is expressed in early osteoarthritis.