Tumor associated antigens and methods of using the same
View Patent ↗The present invention provides methods and compositions for the diagnosis of hyperproliferative disease and autoimmune disease. Tumor associated antigens, nucleic acids encoding them and antibodies to the tumor associated antigens are provided for the diagnosis of hyperproliferative disease, such as, for example, ovarian cancer, breast cancer, lung cancer, colorectal cancer, and other epithelial cancers, and for the diagnosis of autoimmune disease.
1. A method for diagnosis of cancer in a subject, comprising:
obtaining a sample from the subject, the sample comprising antibodies;
contacting the sample with at least one tumor associated polypeptide antigen, the tumor associated antigen selected from the group consisting of
(a) ZFP161,
(b) Ubiquilin-1,
(c) HOX-B6,
(d) YB-1,
(e) Osteonectin,
(f) ILF3, and
(g) a polypeptide comprising an antigenic fragment of any (a) through (f); and
detecting complex formation between the tumor associated antigen and the antibodies in the sample; wherein complex formation indicates a diagnosis of a cancer.
2. The method of claim 1 , wherein the subject is a mammal.
3. The method of claim 2 , wherein the subject is human.
4. The method of claim 1 , wherein the sample is blood, serum, ascites fluid, mucosal fluid, cervical wash, nipple aspirate fluid, stool, urine, saliva, tears, or sputum.
5. The method of claim 4 , wherein the sample comprises serum.
6. The method of claim 1 , wherein the detecting is by Western blot, radioimmunoassay, ELISA, sandwich immunoassay, immunoprecipitation assay, precipitin reaction, gel diffusion precipitin reaction, immunodiffusion assay, agglutination assay, complement-fixation assay, immunoradiometric assay, fluorescent immunoassay or protein A immunoassay.
7. The method of claim 1 , wherein the cancer is epithelial cancer.
8. The method of claim 7 , wherein the epithelial cancer is ovarian cancer, breast cancer, lung cancer or colorectal cancer.
9. The method of claim 1 , further comprising:
comparing the complex formation between the tumor associated antigen and the antibodies in the sample with complex formation of a standard to determine the diagnosis of the cancer.