Viral delivery system for infectious transfer of large genomic DNA inserts
The present invention relates to HSV-based amplicon vectors carrying a genomic DNA fragment, and methods of constructing and using the same. Included within the present invention is a method of converting any large capacity DNA cloning vector, such as a BAC or PAC, into an HSV amplicon or hybrid HSV amplicon using site-specific, or other types of recombination, so that genomic DNA inserts within the BAC or PAC clone can be delivered by infection to a cell, and efficiently expressed. The present invention also relates to a system for the rapid creation of viral vectors carrying transgenes of interest. This aspect of the invention is accomplished through recombination between: (a) a large-capacity cloning vector carrying a viral genome, and (b) a transfer vector containing the transgene of interest. Finally, an expression-ready genomic DNA library is disclosed.
1. A method for converting a large capacity cloning vector into a herpes simplex virus (HSV)-based amplicon, said method comprising recombining:
(a) a large capacity cloning vector comprising a genomic DNA insert; and
(b) an amplicon vector comprising a herpesvirus cleavage/packaging sequence and a herpesvirus origin of replication;
thereby producing an HSV-based amplicon vector comprising said genomic DNA insert; wherein said large capacity cloning vector is a bacterial artificial chromosome (BAC), P1 phage-based vector (PAC), cosmid, yeast artificial chromosome (YAC), or viral based vector.
2. The method of claim 1 , wherein said herpesvirus cleavage/packaging sequence is an HSV-1 cleavage/packaging sequence.
3. The method of claim 1 , wherein said herpesvirus origin of replication is an HSV-1 origin of replication.
4. The method of claim 1 , wherein said herpesvirus cleavage/packaging sequence is an HSV-1 cleavage/packaging sequence, and said herpesvirus origin of replication is an HSV-1 origin of replication.
5. The method of claim 1 , wherein said amplicon vector of (b) further comprises a genetic element from Epstein-Barr virus (EBV).
6. The method of claim 5 , wherein said genetic element from EBV is oriP.
7. The method of claim 1 , wherein said large capacity cloning vector is a bacterial artificial chromosome (BAC).
8. The method of claim 1 , wherein said large capacity cloning vector is a P1 phage-based vector (PAC).
9. The method of claim 1 , wherein said recombining comprises site-specific recombination of (a) and (b) in the presence of a site-specific recombinase.
10. The method of claim 9 , wherein said site-specific recombinase is selected from the group consisting of: P1 bacteriophage CRE, yeast FLP, and yeast R recombinase.
11. The method of claim 9 , wherein said site-specific recombinase is P1 bacteriophage CRE.
12. The method of claim 1 , wherein said recombining comprises homologous recombination of (a) and (b).
13. The method of claim 1 , wherein said recombining comprises ligation of (a) and (b).
14. The method of claim 1 , wherein said genomic DNA insert is 50 to 100 kb in size.
15. The method of claim 1 , wherein said genomic DNA insert is 110 to 150 kb in size.
16. The method of claim 1 , further comprising packaging said HSV-based amplicon vector comprising said genomic DNA insert into an infectious particle.
17. The method of claim 16 , wherein said packaging is accomplished using a helper virus-free system.