GFP expression vector localized in mitochondria

1. A transgenic mouse whose genome comprises a green fluorescent protein (GFP) gene operably linked to a CAG promoter and a cytochrome c oxidase subunit VIII signal sequence, wherein the GFP is specifically expressed in mitochondria.

2. An isolated tissue or cell derived from the transgenic mouse of claim 1 .

3. A screening method for an inducer of apoptosis, comprising the steps of:

(a) contacting the transgenic mouse of claim 1 or the tissue or cell of claim 2 with a test substance; and

(b) confirming whether or not apoptosis is induced on the basis of dynamics of mitochondria visualized by GFP.

4. A screening method for an apoptosis suppressor, comprising the steps of:

(a) contacting the transgenic mouse of claim 1 , or the tissue or cell of claim 2 with a test substance;

(b) treating the mouse or the tissue or cell with the apoptosis inducer; and

(c) confirming whether or not apoptosis is suppressed on the basis of dynamics of mitochondria visualized by GFP.

5. A method for producing a transgenic mouse whose genome comprises GFP expression specifically in the mitochondria, comprising the steps of:

(a) introducing an expression vector comprising a GFP gene operably linked to a CAG promoter and a cytochrome c oxidase subunit VIII signal sequence into totipotent cells;

(b) transferring the totipotent cells obtained in step (a) to the oviduct or uterus of a pseudo-pregnant mouse;

(c) generating progeny from the pseudo-pregnant mouse of step (b);

(d) selecting the transgenic strains, which have GFP transgene expression and fluorescence in isolated tissue or cell samples.

6. The transgenic mouse of claim 1 , wherein the expression vector comprises the DNA fragment shown in SEQ ID NO:9.