Human heme-regulated initiation factor 2-alpha kinase
The present invention provides an isolated nucleic acid sequence encoding a human heme-regulated initiation factor 2 alpha kinase. In addition, the invention provides a method for inhibiting protein synthesis, inducing cellular differentiation, or inhibiting infection in human cells by administering an effective amount of a heme-regulated initiation factor 2 alpha kinase to the cells. Methods are also provided for modulating heme-regulated initiation factor 2 alpha kinase activity and determining the level of heme-regulated initiation factor 2 alpha kinase expression.
1. An isolated protein comprising:
the amino acid sequence of SEQ ID NO:2; or
an amino acid sequence having at least 90% overall identity to the amino acid sequence of SEQ ID NO:2,
wherein the isolated protein has heme-regulated initiation factor 2-alpha kinase activity.
2. An isolated fragment of the protein according to claim 1 , wherein the isolated fragment has heme-regulated initiation factor 2-alpha kinase activity.
3. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain I.
4. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain II.
5. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain III.
6. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain IV.
7. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain V.
8. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain VI.
9. The fragment according to claim 2 , wherein the fragment comprises kinase subdomain VII.
10. The fragment according to claim 2 , wherein the fragment comprises wherein the fragment comprises kinase subdomain VIII.
11. The fragment according to claim 2 , wherein the fragment comprises wherein the fragment comprises kinase subdomain IX.
12. The fragment according to claim 2 , wherein the fragment wherein the fragment comprises kinase subdomain X.
13. The fragment according to claim 2 , wherein the fragment wherein the fragment comprises kinase subdomain XI.
14. The fragment of the protein according to claim 2 , wherein the fragment comprises a heme-binding kinase insertion domain.
15. The fragment according to claim 2 , wherein the fragment comprises heme regulatory motif 1 (HRM 1).
16. The fragment according to claim 2 , wherein the fragment comprises heme regulatory motif 2 (HRM2).