Method of assaying lupus anticoagulant and assay reagent
A monoclonal antibody having inhibitory activity on blood coagulation reactions, which reacts with prothrombin forming a complex with phosphatidylserine but does not react with prothrombin not forming a complex with phosphatidylserine; and a method for measuring inhibitory activity of test plasma on blood coagulation reactions, which comprises the steps of (1) measuring inhibitory activity of the test plasma on the blood coagulation reactions and inhibitory activity of this on the blood coagulation reactions, and (2) comparing the inhibitory activity of the test plasma with the inhibitory activity of this antibody.
1. An antibody produced by 231D cells (FERM BP-7936).
2. A method for comparing an inhibitory activity of plasma from a lupus patient with the inhibitory activity of a standard antibody on a blood coagulation reaction, comprising the steps of:
(1) measuring the inhibitory activity of the plasma on the blood coagulation reaction;
(2) measuring the inhibitory activity of the antibody as defined in claim 1 on the blood coagulation reaction, and
(3) comparing the inhibitory activity of the plasma with the inhibitory activity of the antibody.
3. A method for determining a lupus anticoagulant level in plasma from a lupus patient, comprising the steps of:
(1) measuring the inhibitory activity of the lupus patient's plasma on a blood coagulation reaction;
(2) measuring the inhibitory activity of the antibody as defined in claim 1 on the blood coagulation reaction, and
(3) comparing the inhibitory activity of the lupus patient's plasma with the inhibitory activity of the antibody to express the inhibitory activity of the lupus patient's plasma as a level of the antibody; and
(4) determining a lupus anticoagulant level by using the level of the antibody as an index.
4. The method according to claim 2 , wherein the method for measuring the inhibitory activity on blood coagulation reactions is selected from the group consisting of measurement of prolongation of activated partial thromboplastin time, measurement of prolongation of kaolin clotting time, and measurement of prolongation of dilute Russell's viper venom time, or a combination thereof.
5. A reagent for measuring lupus anticoagulant, which comprises the monoclonal antibody as defined in claim 1 and a carrier.