Streptococcal C5a peptidase vaccine
Novel vaccines for use against β-hemolytic Streptococcus colonization or infection are disclosed. The vaccines contain an immunogenic amount of a variant of streptococcal C5 a peptidase (SCP). Also disclosed is a method of protecting a susceptible mammal against β-hemolytic Streptococcus colonization or infection by administering such a vaccine. Enzymatically inactive SCP, and polynucleotides encoding these SCP proteins are further disclosed.
1. An isolated and purified enzymatically inactive streptococcal C5a peptidase (SCP) which is a variant of a wild-type SCP, wherein the variant is said wild-type SCP having amino acid substitutions at its amino acid residues 130 and 512.
2. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP has reduced binding activity as compared to the wild-type SCP.
3. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP is expressed from an isolated DNA sequence encoding the enzymatically inactive SCP.
4. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP has a catalytic domain.
5. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP comprises contiguous amino acid residues from residue 130 to residue 512.
6. The enzymatically inactive SCP of claim 1 , wherein the substitutions at the amino acid residues 130 and 512 are conserved amino acid substitutions.
7. The enzymatically inactive SCP of claim 6 , wherein the amino acid residues 130 and 512 are substituted with alanine.
8. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP varies from the wild-type SCP in that it does not contain a signal sequence.
9. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP varies from the wild-type SCP in that it does not contain a cell wall insert.
10. The enzymatically inactive SCP of claim 1 , wherein the enzymatically inactive SCP is from group A Streprococcus , group B Streprococcus , group C Streprococcus , or group G Streprococcus.
11. The euzymatically inactive SCP of claim 10 , wherein the enzymatically inactive SCP is from group A Streprococcus .