Composition for delivery of a mitochondrial genome to a cell
The invention provides composition and methods for delivering a composition according to the invention comprising a wild-type (wt) mitochondrial DNA (mtDNA) molecule to a mammalian cell. The wt-mtDNA molecule is a functional mtDNA molecule that includes the entire mitochondrial genome. The wt-mtDNA molecule in the composition of the invention has a mitochondrial leader sequence (MLS) peptide attached to facilitate the uptake of the mtDNA molecule into a mitochondrion of the mammalian cell. The delivery of the wt-mtDNA/MLS peptide complex is used as a general replenishment therapy for conditions attributed to mtDNA defects, independent of the specific defect in the mtDNA.
1. A composition for delivering a wild-type mitochondrial DNA genome to a mammalian cell, said composition comprising:
a wild-type mammalian mitochondrial DNA genome molecule;
a mitochondrial leader sequence peptide attached to said mitochondrial DNA genome molecule; and
a carrier vehicle comprising two delocalized cationic centers separated by a hydrocarbon chain.
2. The composition of claim 1 , wherein said mitochondrial leader sequence peptide is attached in a non-coding region.
3. The composition of claim 1 , wherein said mitochondrial leader sequence peptide is attached in a region of the membrane attachment site from nucleotide position 15925 to nucleotide position 499 of said mitochondrial DNA genome molecule.
4. The composition of claim 1 , wherein said mitochondrial leader sequence peptide is attached in a region of the D-loop from nucleotide position 16024 to nucleotide position 576 of said mitochondrial DNA genome molecule.
5. The composition of claim 1 , wherein said wild-type mammalian mitochondrial DNA genome molecule originates from a mammalian cell.
6. The composition of claim 1 , wherein said wild-type mammalian mitochondrial DNA genome molecule is isolated from cell culture.
7. The composition of claim 1 , wherein said wild-type mammalian mitochondrial DNA genome molecule is chemically synthesized.