Composition and method for modulating dendritic cell-T cell interaction
The present invention relates to the use of a compound that binds to a C-type lectin on the surface of a dendritic cell, in the preparation of a composition for modulating, in particular reducing, the immune response in an animal, in particular a human or another mammal. The composition in particular modulates the interactions between a dendritic cell and a T-cell, more specifically between a C-type lectin on the surface of a dendritic cell and an ICAM receptor on the surface of a T-cell. The compositions can be used for preventing/inhibiting immune responses to specific antigens, for inducing tolerance, for immunotherapy, for immunosuppression, for the treatment of autoimmune diseases, and the treatment of allergy. The compound that binds to a C-type lectin is preferably chosen from mannose, fucose, plant lectins, antibiotics, sugars, proteins or antibodies against C-type lectins. The invention also relates to such antibodies.
1. A method for increasing an immune response in an animal, comprising administering a compound which binds to a protein with the amino acid sequence of SEQ ID NO: 2 on the surface of a dendritic cell, wherein said compound is bound to an antigen.
2. The method of claim 1 wherein said animal is a mammal.
3. The method of claim 2 wherein said mammal is a human.
4. The method of claim 1 wherein said antigen is bound to said compound by a) covalent binding, b) ligand-ligand interaction, c) complexing, d) ligation, or e) expression of a fusion protein comprising said antigen and said compound.
5. The method of claim 1 wherein said antigen is a cancer antigen.
6. The method of claim 5 wherein said method generates an immune response against tumor cells containing or expressing said cancer antigen.
7. The method of claim 1 wherein said compound is selected from the group consisting of a mannose carbohydrate, a fucose carbohydrate, an antibiotic, a sugar, a protein, and an antibody.
8. The method of claim 7 wherein said mannose carbohydrate is mannan or D-mannose.
9. The method of claim 7 wherein said fucose carbohydrate is L-fucose.
10. The method of claim 7 wherein said antibiotic is pradimicin A.
11. The method of claim 7 wherein said sugar is selected from the group consisting of N-acetyl-D-glucosamine and galactose.
12. The method of claim 7 wherein said protein is selected from the group consisting of gp120, analogs of gp120 and fragments of gp120.
13. The method of claim 7 wherein said antibody is a monoclonal antibody.
14. The method of claim 7 wherein said antibody is selected from the group consisting of i) an antibody produced by hybridoma ECACC accession number 99040818 and ii) an antibody produced by hybridoma ECACC accession number 99040819.
15. A method for generating an immune response against tumor cells in an animal, the method comprising administering a compound which binds to a protein with the amino acid sequence of SEQ ID NO: 2 on the surface of a dendritic cell, the compound having at least a portion of a cancer antigen attached thereto.