IP Library › Granted Patent US 7,285,650
Granted Patent B2
US 7,285,650 · App. 10/303,426 · Granted Oct 23, 2007

Cloning and sequencing of the allergen Dac g5 of

Assignees: Seita Groupe Altadis; Stallergenes
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Quick Facts
Patent No.
US 7,285,650
App. No.
10/303,426
Granted
Oct 23, 2007
Kind
B2
Abstract

A purified nucleic acid molecule comprising a nucleotide sequence coding for allergen Dac g5 having amino acid sequence SEQ ID NO. 2, a derivative or a fragment thereof.

Claims (17)

1. A purified nucleic acid molecule comprising a nucleotide sequence coding for allergen Dac g 5 comprising the amino acid sequence selected from the group consisting of SEQ ID NO. 2, SEQ ID NO. 4, SEQ ID NO. 6, and SEQ ID NO. 8.

2. The purified nucleic acid molecule according to claim 1 , comprising the nucleotide sequence coding for an allergen Dac g 5 comprising amino acid sequence SEQ ID NO. 2.

3. The purified nucleic acid molecule according to claim 1 , comprising the nucleotide sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5 and SEQ ID NO. 7.

4. The purified nucleic acid molecule according to claim 2 , comprising the nucleotide sequence SEQ ID NO. 1.

5. A recombinant nucleic acid molecule comprising the nucleic acid molecule according to any one of claims 1 to 4 , a promoter bound to said nucleic acid molecule, a selection gene placed under control of a promoter, and a termination sequence downstream of said nucleic acid molecule.

6. An expression vector comprising an origin of eukaryote or prokaryote replication, and the recombinant nucleic acid molecule according to claim 5 .

7. An isolated eukaryote or prokaryote host transformed by the nucleic acid molecule according to claim 5 .

8. A eukaryote or prokaryote host transformed by an expression vector according to claim 6 .

9. A tobacco plant having incorporated in its genome the nucleic acid molecule according to any one of claims 1 to 4 , placed under regulation sequence control in a manner to express allergen Dac g 5 in a selected part of the plant.

10. A tobacco plant cell suspension, whose cells have incorporated in their genome the nucleic acid molecule according to any one of claims 1 to 4 , placed under regulation sequence control in a manner to express allergen Dac g 5 in said cells.

11. A yeast having incorporated in its genome the nucleic acid molecule according to any one of claims 1 to 4 , placed under regulation sequence control in a manner to express allergen Dac g 5.

12. A process for producing recombinant allergen Dac g 5 comprising:

culturing a prokaryote or eukaryote organism transformed by the nucleic acid molecule according to claim 5 under conditions and for a sufficient length of time to enable expression of said allergen, and

isolating allergens produced from the transformed organisms.

13. A process for producing recombinant allergen Dac g 5 comprising:

culturing a prokaryote or eukaryote organism transformed by the expression vector according to claim 6 under conditions and for a sufficient length of time to enable expression of said allergen, and

isolating allergens produced from the transformed organisms.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 22, 2015
From: STALLERGENES SA
To: STALLERGENES
Reel/Frame 036852/0573 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 10, 2003
From: VAN REE, RONALD; VAN OORT, ERICA; BONNEAU, CAROLINE; FAYE, LOIC; GOMORD, VERONIQUE
To: SEITA GROUPE ALTADIS, A FRENCH CORPORATION; STALLERGENES, A FRENCH CORPORATION
Reel/Frame 013739/0644 →
Priority Claims (1)
FR 00 06857 · May 29, 2000 · national
Continuity (2)
Continuation PCTFR010166600 · May 29, 2001
Related Publication 20030147880A1 · Aug 7, 2003