Expression of human serum albumin (HSA) in monocot seeds
A method of producing human serum albumin (HSA) in monocot plant seeds, by transforming a monocot plant cell with a chimeric gene containing a promoter from the gene of a maturation-specific monocot plant storage protein, a first DNA sequence operably linked to the promoter and encoding a signal sequence, and a second DNA sequence linked in translation frame with the first DNA sequence and encoding HSA, where the first DNA sequence and the second DNA sequence together encode a fusion protein containing an N-terminal signal sequence and the HSA; growing a monocot plant from the transformed monocot plant cell for a time sufficient to produce seeds containing the HSA; and harvesting the seeds from the plant.
1. A method of producing human serum albumin (HSA) in monocot plant seeds, comprising the steps of:
(a) transforming a monocot plant cell with a chimeric gene comprising
(i) a promoter from the gene of a maturation-specific monocot plant storage protein, wherein the promoter comprises SEQ ID NO.:8,
(ii) a first DNA sequence, operably linked to the promoter, encoding a signal sequence, and
(iii) a second DNA sequence, linked in translation frame with the first DNA sequence, encoding HSA, wherein the first DNA sequence and the second DNA sequence together encode a fusion protein comprising an N-terminal signal sequence and the HSA;
(b) growing a monocot plant from the transformed monocot plant cell for a time sufficient to produce seeds containing the HSA; and
(c) harvesting the seeds from the plant,
wherein the HSA constitutes at least about 10% of the total soluble protein in the harvested seeds.
2. The method of claim 1 , further comprising purifying the HSA from the harvested seeds.