Diagnostic methods for age related macular degeneration
View Patent ↗Diagnostic methods for identifying a test subject who has or is at risk of developing age-related macular degeneration (AMD) or an analogous disease associated with oxidation of DHA-containing lipids are provided. In one aspect, the methods comprise: assaying for the presence of elevated levels of 2-(ω-carboxyethyl) pyrrole (CEP) adducts in a bodily fluid which has been obtained from the test subject. In a preferred embodiment, such methods comprise providing an antibody that is immunospecific for CEP, contacting a bodily fluid from the subject with the anti-CEP antibody, and assaying for the formation of a complex between the antibody and an antigen in the sample. In another aspect, the methods comprise assaying for the presence of elevated levels of an antibody that binds to or is immunospecific for a CEP adduct in the bodily fluid of the test subject. The present invention also relates to CEP protein and peptide adducts, an antibody reactive with a CEP adduct and a diagnostic kit comprising such antibody.
1. A method of diagnosing age-related macular degeneration (AMD) in a test subject, comprising
determining the level of anti-2-(ω-carboxyethyl) pyrrole (CEP) antibodies in a bodily fluid obtained from the test subject, wherein the presence of an elevated level of anti-CEP antibodies in the test subject's bodily fluid as compared to a control value based on the level of anti-CEP antibodies found in a corresponding bodily fluid obtained from control subjects indicates that the test subject is more likely to have AMD than a test subject whose levels of anti-CEP antibodies are equal to or less than the control value.
2. The method of claim 1 , wherein the bodily fluid is whole blood, serum, or plasma.
3. The method of claim 1 , wherein levels of anti-CEP antibodies in the bodily fluid are determined by contacting the bodily fluid with a CEP adduct and assaying for the formation of an antigen-antibody complex between the CEP adduct and antibodies in the bodily fluid.