Patent
Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of the Brassica juncea B genome. Two B. juncea AHAS gene sequences (BjAHAS-a and BjAHAS-b) and one B. nigra AHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutant B. juncea lines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.
1. A seed of the Brassica juncea line J04E-0044, wherein a representative sample of the seed was deposited under ATCC Accession No. PTA-6324.
2. A B. juncea plant, or a part thereof, produced by growing the seed of claim 1 .
3. A tissue culture of regenerable cells produced from the plant of claim 2 , wherein the regenerable cells are selected from the group consisting of protoplasts, callus cells, embryos, immature embryos, meristematic cells, microspores, pollen, leaves, anthers, roots, root tips, flowers, and stalks.
4. A B. juncea plant regenerated from the tissue culture of claim 3 , wherein the B. juncea plant has all of the physiological and morphological characteristics of the B. juncea line designated JO4L-0044.
5. The regenerated B. juncea plant, of claim 4 or a part thereof, wherein the regenerated B. juncea plant or the part thereof has been transformed so that the genetic material of the regenerated B. juncea plant or the part thereof contains one or more transgenes operably linked to one or more promoters.
6. A method for producing a Brassica plant that contains in its genetic material one or more transgenes, wherein the method comprises crossing the regenerated B. juncea plant of claim 5 with either a second plant of another Brassica variety comprising a transgene or a transformed Brassica plant, so that the genetic material of the progeny that result from the cross contains the transgene(s) operably linked to a promoter.
7. A Brassica plant, or a part thereof, produced by the method of claim 6 .
8. A method for developing a Brassica plant in a Brassica plant breeding program using plant breeding techniques which include using a Brassica plant, or a part thereof, as the source of plant breeding material wherein the method comprises using the Brassica plant of claim 2 , or a part thereof, as a source of said breeding material.
9. A method for producing an F1 hybrid Brassica seed, wherein the method comprises crossing the plant of claim 2 with a different Brassica plant and harvesting the resultant F1 hybrid Brassica seed.
10. A hybrid Brassica seed produced by the method of claim 9 .
11. A hybrid Brassica plant, or a part thereof, produced by growing said hybrid seed of claim 10 .
12. A progeny plant or part thereof obtained from any line derived from a cross using the plant of claim 2 wherein said progeny plant or part thereof is herbicide-resistant and comprises a BjAHAS-b gene comprising the nucleotide sequence set forth in SEQ ID NO: 14.
13. The plant of claim 12 , wherein the herbicide resistance is to an imidazolinone herbicide.
14. A method of producing a Brassica plant wherein the method comprises:
a) crossing the plant of claim 2 with a Brassica plant to form F1 progeny plants;
b) propagating the progeny plants; and
c) selecting from the progeny plants a plant that is genetically stable and generates seeds having mutant allele BjAHAS-bR, wherein said selected plant is herbicide resistant.
15. The method of claim 14 , wherein said propagating is selected from the group consisting of self-pollination and the development of doubled haploid plants.
16. The method of claim 14 , wherein the herbicide is an imidazolinone herbicide.