Microfluidic device
The object of the present invention is to provide a production process of a microfluidic device in which a porous resin layer, which is capable of optimally fixing a large amount of enzyme, antigen or other protein or catalyst on the inner surface of a minute channel of a microfluidic device without obstructing said channel, is formed at a uniform thickness on the surface of said channel. In the present invention, by preliminarily forming a porous resin layer having a large number of pores in its surface on a substrate, and forming an indentation having a porous resin layer on its bottom surface by using an activating energy beam-curable composition on said porous resin layer, followed by forming a channel by adhering a member serving as a cover to said indentation, a porous resin layer can easily be formed at a uniform thickness on the surface of said channel.
1. A microfluidic device comprising a substrate, a porous resin layer having a three-dimensional mesh structure, a channel and a cover; wherein, said microfluidic device (I) has said porous resin layer in the upper portion of the substrate, (II) said porous resin layer is filled with a curable resin of an activating energy beam-curable resin composition (X) impregnated excluding the channel portion, and (III) the channel has wall surfaces consisting of a porous resin layer having a three-dimensional mesh structure that is not filled with activating energy beam-curable resin composition (X), a curable resin layer of the activating energy beam-curable resin composition (X) formed in the upper portion of the porous resin layer having a three-dimensional mesh structure filled with the curable resin of activating energy beam-curable resin composition (X), and a cover, and is formed into the form of a cavity.
2. The microfluidic device according to claim 1 , wherein the porous resin layer having a three-dimensional mesh structure is composed of an activating energy beam-curable resin composition.
3. The microfluidic device according to claim 1 , wherein, in a cross-section in the direction perpendicular to the direction of flow of a fluid in a portion of the channel having a porous resin layer having a three-dimensional mesh structure, if an arbitrary point in said cross-section is designated as x, the portion of the porous resin layer having a three-dimensional mesh structure that is closest to said arbitrary point in terms of straight line distance is designated as y, the straight line distance between x and y is designated as r, and the maximum distance that r is able to have in said cross-section is designated as r max , then said r max is within the range of 1 to 50 μm.
4. The microfluidic device according to claim 1 , wherein the shape of a cross-section of the channel in the direction perpendicular to the direction of fluid flow is rectangular or trapezoidal.
5. The microfluidic device according to claim 4 , wherein the porous resin layer having a three-dimensional mesh structure is formed on only one inner wall of the channel, and the average distance from said porous resin layer to the opposing inner wall is within the range of 1 to 50 μm.
6. The microfluidic device according to claim 1 , wherein the thickness of the porous resin layer having a three-dimensional mesh structure is within the range of 0.5 to 30 μm.
7. The microfluidic device according to claim 1 , wherein the mean pore diameter of the porous resin layer having a three-dimensional mesh structure is within the range of 0.05 to 3 μm.
8. The microfluidic device according to claim 1 , wherein the porous resin layer having a three-dimensional mesh structure is a porous resin layer to which a probe having affinity for an analysis target substance is fixed.
9. The microfluidic device according to claim 8 , wherein the probe is an oligonucleotide.