Method for efficiently determining a DNA strand break
The present invention is directed to directly measure distribution in vivo and the frequency of generation in vivo of DNA strand breaks which induce cell death and mutations. The present inventors accomplished the present invention by providing a method for detecting a DNA strand break in a sample, which comprises a step of binding a PprA protein derived from Deinococcus radiodurans to a DNA strand break and a step of detecting the PprA protein which is bound to the DNA strand break; as well as by providing a kit for detecting a DNA strand break in a sample which comprises PprA proteins derived from Deinococcus radiodurans and a means for detecting a PprA protein which is bound to a DNA strand break.
1. A method for detecting in situ a DNA strand break, comprising:
binding a PprA protein having an amino acid sequence of SEQ ID NO: 1, which is obtained from Deinococcus radiodurans , to a DNA strand break; and
detecting in situ the PprA protein bound to the DNA strand break using an antibody or a fragment thereof which specifically binds to the PprA protein.
2. The method for detecting in situ a DNA strand break according to claim 1 , wherein the antibody is a polyclonal antibody or a monoclonal antibody or the fragment thereof which is selected from the group consisting of Fab, F(ab′) 2 , and Fab′.
3. A kit for detecting in situ a DNA strand break, comprising:
an amount of PprA protein having an amino acid sequence of SEQ ID NO: 1, which is obtained from Deinococcus radiodurans ; and
an antibody or a fragment thereof which specifically binds to the PprA protein.
4. The kit for detecting in situ a DNA strand break according to claim 3 , wherein the antibody is a polyclonal antibody or a monoclonal antibody or the fragment thereof which is selected from the group consisting of Fab, F(ab′) 2 , and Fab′.