Compositions comprising mixtures of positional PEG isomers of PEGylated G-CSF
A method for increasing the stability and uniformity of a PEGylated G-CSF polypeptide having at least one PEG moiety attached to the epsilon amino group of a lysine residue or the N-terminal amino group and at least one PEG moiety attached to a hydroxyl group, comprising subjecting the polypeptide to an elevated pH of above 8.0 for a period of time suitable to remove PEG moieties attached to a hydroxyl group, and reducing the pH to about 8.0 or lower; as well as PEGylated G-CSF polypeptides and compositions produced according to the method and methods for increasing neutrophil levels in a patient using the PEGylated G-CSF polypeptides and compositions.
1. A composition comprising a mixture of positional PEG isomers of a PEGylated G-CSF polypeptide, wherein the polypeptide comprises an amino acid sequence consisting of SEQ ID NO:1 with the substitutions K16R, K34R, K40R, T105K and S159K, and wherein at least about 80% of the mixture of positional PEG isomers consists of two positional PEG isomers each consisting of three attached PEG moieties,
wherein one of the two isomers has PEG moieties attached at the N-terminal, Lys23 and Lys159 and the other of the two isomers has PEG moieties attached at the N-terminal, Lys105 and Lys159, and wherein the attached PEG moieties are about 5 kDa molecular weight.
2. The composition of claim 1 , wherein at least about 85% of the mixture of positional PEG isomers consists of the two isomers.
3. The composition of claim 1 , wherein at least about 90% of the mixture of positional PEG isomers consists of the two isomers.
4. A composition comprising
a pharmaceutically acceptable carrier, and
a mixture of positional PEG isomers of a PEGylated G-CSF polypeptide, wherein the polypeptide comprises an amino acid sequence consisting of SEQ ID NO:1 with the substitutions K16R, K34R, K40R. T105K and S159K, and wherein at least about 80% of the mixture of positional PEG isomers consists of two positional PEG isomers each consisting of three attached PEG moieties,
wherein one of the two isomers has PEG moieties attached at the N-terminal, Lys23 and Lys159 and the other of the two isomers has PEG moieties attached at the N-terminal, Lys105 and Lys159, and wherein the attached PEG moieties are about 5 kDa molecular weight.
5. The composition of claim 4 , wherein at least about 85% of the mixture of positional PEG isomers consists of the two isomers.
6. The composition of claim 4 , wherein at least about 90% of the mixture of positional PEG isomers consists of the two isomers.