Process for the fermentative production of S-adenosylmethionine
A microorganism strain secreting S-adenosylmethionine having increased activity of the cmr (mdfA) gene product is provided.
1. An isolated E. coli cell which secrets S-adenosylmethionine as a result of a modification in said cell to increase the activity of the poypeptide of SEQ ID NO: 2 compared to the activity of the polypeptide of SEQ ID NO: 2 in the corresponding E. coli cell prior to modification, wherein said increase in ativity is due to over-expression of a nucleic acid encoding the polypeptide of SEQ ID NO: 2 by placing said nucleic acid under the control of a strong heterologous promoter.
2. The isolated E. coli cell of claim 1 wherein said E. coli cell exhibits at least 2 fold increased activity of the polypeptide of SEQ ID NO: 2 compared to the activity of the polypeptide SEQ ID NO: 2 in the corresponding E. coli cell prior to modification.
3. The isolated E. coli cell of claim 1 wherein said E. coli cell exhibits at least 5 fold increased activity of the polypeptide of SEQ ID NO: 2 compared to the activity of the polypeptide of SEQ ID NO: 2 in the corresponding E. coli cell prior to modification.
4. An isolated E. coli cell which secrets 5-adenosylmethionine as a result of a modification in said cell to increase the activity of the polypeptide of SEQ ID NO: 2 compared to the activity of the polypeptide of SEQ ID NO: 2 in the corresponding E. coli cell prior to modification, wherein said increase in activity is due to an increase in the copy number of a nucleic acid encoding the polypeptide of SEQ ID NO: 2.
5. The isolated E. coli cell of claim 4 wherein said E. coli cell exhibits at least 5 fold increased activity of the polypeptide of SEQ ID NO: 2 compared to the activity of the polypeptide of SEQ ID NO: 2 in the corresponding E. coli cell prior to modification.
6. The isolated E. coli cell of claim 4 wherein E. coli cell exhibits at least 5 fold increased activity of the polypeptide of SEQ ID NO: 2 compared to the activity of the polypeptide of SEQ ID NO: 2 in the corresponding E.coli cell prior to modification.