Patent
An isolated microbial consortium is described that includes a first microbial consortium having Thermacetogenium phaeum to metabolize a complex hydrocarbon substrate into metabolic products comprising an acetate compound. The consortium also includes a second microbial consortium having a methanogen to convert the acetate compound into a final product that includes methane. Also, a method of increasing production of materials with enhanced hydrogen content. The method includes isolating Thermacetogenium phaeum from geologic formation water, culturing the isolated Thermacetogenium phaeum to increase the Thermacetogenium phaeum population, and introducing a consortium of the cultured Thermacetogenium phaeum , which may include spores of Thermacetogenium phaeum , into a hydrocarbon formation containing a complex hydrocarbon substrate.
1. A method of stimulating biological activity of Thermacetogenium phaeum in a geologic formation, the method comprising:
detecting the Thermacetogenium phaeum in the geologic formation;
measuring one or more environmental characteristics of the geologic formation, and determining a composition of carbonaceous material in the formation; and
adding an amendment to the formation based on the measurements of the environmental characteristics and the composition of the carbonaceous material.
2. The method of claim 1 , wherein the amendment increases a rate at which the Thermacetogenium phaeum metabolizes the carbonaceous material into metabolic products comprising an acetate compound.
3. The method of claim 1 , wherein the amendment increases population growth of the Thermacetogenium phaeum in the geologic formation.
4. The method of claim 1 , wherein the amendment comprises adding a growth stimulant to the formation.
5. The method of claim 4 , wherein the growth stimulant comprises sodium pyruvate.
6. The method of claim 1 , wherein the amendment comprises adding a nutrient to the formation.
7. The method of claim 6 , wherein the nutrient comprises methanol, ethanol, n-propanol, n-butanol, 2,3-butanediol, ethanolamine, 3,4,5-trimethoxybenzoate, syringate, vanillate, glycine, cysteine, formate, hydrogen, or carbon dioxide.