Protease regulator screening assay
A polypeptide containing an anchor region, a protease recognition site, and a detectable signal region can be produced recombinantly and directly attached to a solid support. The polypeptide is useful for screening protease regulators, especially protease inhibitors.
1. A method of forming a substrate for use in a screening assay for protease regulators comprising binding a polypeptide consisting of a polyamino acid sequence to a solid support comprising a protein via a protein-protein interaction having a K d of no more than 10 −8 , wherein the polypeptide comprises:
an anchor region,
a protease recognition site which comprises
a) a cleavable peptide bond, and
b) at least 3 naturally adjacent amino acids on each side of the cleavable peptide bond; and
a detectable signal region, wherein the polypeptide binds to the protein via the anchor region.
2. The method of claim 1 wherein the protein is an antibody.