AAV5 nucleic acids
The present invention provides an adeno-associated virus 5 (AAV5) virus and vectors and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using the AAV5 vectors and particles.
1. An isolated nucleic acid comprising an adeno-associated virus 5 (AAV5)-specific nucleic acid or a nucleic acid encoding an AAV5-specific protein, wherein the isolated nucleic acid further comprises a heterologous nucleic acid.
2. The isolated nucleic acid of claim 1 , wherein the AAV5-specific protein is an AAV5 Rep protein.
3. The isolated nucleic acid of claim 1 , wherein the AAV5-specific protein is an AAV5 capsid protein.
4. The isolated nucleic acid of claim 1 , wherein the AAV5-specific nucleic acid is an AAV5 inverted terminal repeat (ITR).
5. The isolated nucleic acid of claim 1 , wherein the AAV5-specific nucleic acid is an AAV5 p5 promoter.
6. The nucleic acid of claim 2 , wherein the AAV5 Rep protein is Rep40.
7. The nucleic acid of claim 2 , wherein the AAV5 Rep protein is Rep52.
8. The nucleic acid of claim 2 , wherein the AAV5 Rep protein is Rep68.
9. The nucleic acid of claim 2 , wherein the AAV5 Rep protein is Rep78.
10. The nucleic acid of claim 2 , wherein the AAV5 Rep protein is Rep52.
11. The nucleic acid of claim 6 , wherein the Rep40 protein comprises the amino acid sequence set forth in SEQ ID NO:12.
12. The nucleic acid of claim 7 , wherein the Rep52 protein comprises the amino acid sequence set forth in SEQ ID NO:2.
13. The nucleic acid of claim 8 , wherein the Rep68 protein comprises the amino acid sequence set forth in SEQ ID NO: 14.
14. The nucleic acid of claim 9 , wherein the Rep78 protein comprises the amino acid sequence set forth in SEQ ID NO:3.
15. The nucleic acid of claim 10 , wherein the Rep52 protein comprises the amino acid sequence set forth in SEQ ID NO:2.
16. The nucleic acid of claim 3 , wherein the AAV5 capsid protein is VP1.
17. The nucleic acid of claim 3 , wherein the AAV5 Capsid protein is VP2.
18. The nucleic acid of claim 3 , wherein the AAV5 Capsid protein is VP3.
19. The nucleic acid of claim 16 , wherein the VP1 capsid protein comprises the amino acid sequence set forth in SEQ ID NO:4.
20. The nucleic acid of claim 17 , wherein the VP2 capsid protein comprises the amino acid sequence set forth in SEQ ID NO:5.
21. The nucleic acid of claim 18 , wherein the VP3 capsid protein comprises the amino acid sequence set forth in SEQ ID NO:6.
22. The nucleic acid of claim 4 , wherein the AAV5 ITR comprises the nucleic acid sequence set forth in SEQ ID NO:19.
23. The nucleic acid of claim 4 , wherein the AAV5 ITR comprises the nucleic acid sequence set forth in SEQ ID NO:20.
24. The nucleic acid of claim 4 , wherein the AAV5 ITR comprises the nucleic acid sequence set forth in SEQ ID NO:23.
25. The nucleic acid of claim 4 , wherein the AAV5 ITR comprises the nucleic acid sequence set forth in SEQ ID NO:21.
26. The nucleic acid of claim 5 , wherein the AAV5 p5 promoter has the nucleic acid sequence set forth in SEQ ID NO:18.