Method of enhancing myogenesis by electrical stimulation
The present invention provides a method for enhancing regeneration of the myocardium. The method comprises the steps of applying electrical stimulation to an injury site in the myocardium. The method can be used in combination with implantation of myogenic cells into the injury site. The electrical stimulation may be applied before or after the implantation.
1. A method of enhancing myogenesis in myocardium comprising the steps of:
identifying an injury site in the myocardium; and applying electrical stimulation to the site, wherein the application of the electrical stimulation causes myogenesis, wherein the electrical stimulation is provided as 30 pulses per second with a voltage of 3.5 mV, and successively alternating currents of 700 to 1500 picoamps for one minute, 200 picoamps for 10 seconds, and again with 700 to 1500 picoamps for one minute.
2. The method of claim 1 , wherein the electrical stimulation recruits stem cells to the injury site.
3. The method of claim 1 , wherein the direction of the current of the electrostimulation is periodically reversed.
4. The method of claim 1 , further comprising the step of implanting myogenic cells into the site of injury before or after application of electrical stimulation.
5. The method of claim 4 , wherein the myogenic cells are first electrostimulated in culture.
6. The method of claim 5 , wherein the cells are electrostimulated with 15 mV and a current of 500 picoamps at 70 pulses per minute.
7. The method of claim 5 , wherein the cells are plated on collagen coated substrates.
8. The method of claim 5 , wherein the cells are stretched 0.2 mms at 70 beats per minute.