Human neuronal attachment factor-1 antibodies
A human F-spondin-like protein and DNA (RNA) encoding such protein and a procedure for producing such protein by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for treating spinal cord injuries and damage to peripheral nerves by promoting neural-cell adhesion and neurite extension, inhibiting tumor metastases and tumor angiogenesis, and stimulating wound repair. Antagonists are also disclosed which may be utilized to prevent malaria. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention for detecting diseases, for example, cancer, are also disclosed.
1. An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of:
(a) a protein whose amino acid sequence consists of amino acid residues 1 to 331 of SEQ ID NO:2;
(b) a protein whose amino acid sequence consists of amino acid residues 2 to 331 of SEQ ID NO:2;
(c) a protein whose amino acid sequence consists of amino acid residues 24 to 331 of SEQ ID NO:2;
(d) a protein whose amino acid sequence consists of amino acid residues 27 to 331 of SEQ ID NO:2;
(e) a protein whose amino acid sequence consists of amino acid residues 284 to 330 of SEQ ID NO:2;
(f) a protein whose amino acid sequence consists of amino acid residues 75 to 100 of SEQ ID NO:2;
(g) a protein whose amino acid sequence consists of amino acid residues 168 to 180 of SEQ ID NO:2;
(h) a protein whose amino acid sequence consists of amino acid residues 204 to 226 of SEQ ID NO:2;
(i) a protein whose amino acid sequence consists of amino acid residues 258 to 281 of SEQ ID NO:2;
(j) a protein whose amino acid sequence consists of amino acid residues 291 to 327 of SEQ ID NO:2;
(k) a protein whose amino acid sequence consists of the amino acid sequence of the full-length polypeptide encoded by the cDNA contained in ATCC Deposit No. 97343; and
(l) a protein whose amino acid sequence consists of the mature form of NAF-1 encoded by the cDNA contained in ATCC Deposit No. 97343.
2. The antibody or fragment thereof of claim 1 that specifically binds protein (a).
3. The antibody or fragment thereof of claim 1 that specifically binds protein (b).
4. The antibody or fragment thereof of claim 1 that specifically binds protein (c).
5. The antibody or fragment thereof of claim 1 that specifically binds protein (d).
6. The antibody or fragment thereof of claim 1 that specifically binds protein (e).
7. The antibody or fragment thereof of claim 1 that specifically binds protein (f).
8. The antibody or fragment thereof of claim 1 that specifically binds protein (g).
9. The antibody or fragment thereof of claim 1 that specifically binds protein (h).
10. The antibody or fragment thereof of claim 1 that specifically binds protein (i).
11. The antibody or fragment thereof of claim 1 that specifically binds protein (j).
12. The antibody or fragment thereof of claim 1 that specifically binds protein (k).
13. The antibody or fragment thereof of claim 1 that specifically binds protein (l).
14. The antibody or fragment thereof of claim 1 which is a monoclonal antibody.
15. The antibody or fragment thereof of claim 1 which is a polyclonal antibody.
16. The antibody or fragment thereof of claim 1 which is selected from the group consisting of:
(a) a chimeric antibody;
(b) a humanized antibody; and
(c) a Fab fragment.
17. A composition comprising the antibody or fragment thereof of claim 1 and a carrier.
18. An isolated cell that produces the antibody or fragment thereof of claim 1 .
19. A hybridoma that produces the antibody or fragment thereof of claim 1 .
20. An isolated antibody or fragment thereof that specifically binds a NAF-1 protein purified from a cell culture, wherein said NAF-1 protein is encoded by a polynucleotide encoding amino acids 1 to 331 of SEQ ID NO:2.
21. The antibody or fragment thereof of claim 20 which is a monoclonal antibody.
22. The antibody or fragment thereof of claim 20 which is a polyclonal antibody.
23. The antibody or fragment thereof of claim 20 which is selected from the group consisting of:
(a) a chimeric antibody;
(b) a humanized antibody; and
(c) a Fab fragment.
24. A composition comprising the antibody or fragment thereof of claim 20 and a carrier.
25. An isolated cell that produces the antibody or fragment thereof of claim 20 .
26. A hybridoma that produces the antibody or fragment thereof of claim 20 .