Copper-inducible promoter system
The present invention comprises a copper-inducible promoter system of yeast for the controlled expression of recombinant genes. The invention comprises a promoter element comprising at least two tandemly-repeated MRE sequences and a minimal TATA element from the 35S-promoter of the cauliflower mosaic virus.
1. An isolated promoter comprising not more than one MRE duplex consisting of two tandemly-repeated MRE sequences wherein each MRE sequence comprises an ACE1 binding site.
2. The promoter of claim 1 further comprising a minimal TATA element from a 35S-promoter of a cauliflower mosaic virus.
3. The promoter of claim 2 wherein said promoter is inserted into a vector.
4. The promoter of claim 3 wherein said vector comprises pGEM3zf.
5. The promoter of claim 4 wherein said promoter is placed between the EcoRI and BamHI sites of pGEM3zf.
6. The promoter of claim 2 comprising SEQ ID NO:5.
7. The promoter of claim 2 consisting essentially of SEQ ID NO:5.
8. An isolated promoter comprising not more than one MRE duplex consisting of two tandemly-repeated MRE sequences wherein each MRE sequence comprises an ACE1 binding site; and
a minimal TATA element from a 35S-promoter of a cauliflower mosaic virus, said TATA element placed immediately downstream of said MRE duplex; and
wherein said MRE duplex and said TATA element are disposed in a PGEM3zf vector between the EcoRI and BamHI sites of said vector.