Method for producing yeast expressed HPV types 6 and 16 capsid proteins
Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production of conformational antibodies against both L1 proteins upon administration to mice.
1. A method for producing VLPs comprising capsid proteins from at least two types of viruses, said method comprising
a) cloning said capsid proteins into expression cassettes comprising the same promoters and termination sequences, wherein said capsid proteins comprise a first late 1 (L 1 ) capsid protein from one type of virus and a second L 1 capsid protein from a second type of virus; and
b) expressing said cassettes in the same host cell.
2. The method of claim 1 wherein the host cell is a yeast cell.
3. The method of claim 2 wherein the yeast is Saccharomyces cerevisiae.
4. The method of claim 1 wherein said viruses are different types of HPV.
5. The method of claim 1 further comprising L2 capsid proteins.
6. The method of claim 5 wherein said L1 protein expression cassettes are cloned into non-integrative vectors, and said L2 protein expression cassettes are cloned into integrative vectors.
7. The method of claim 6 wherein the non-integrative vector is pBS24. 1.
8. The method of claim 6 wherein the integrative vector is pUC8.