Lamprey GnRH-III polypeptides and methods of making thereof
Lamprey GnRH-III polypeptides for the four species of fish lamprey are disclosed. Also disclosed is a procedure for producing such polypeptides by recombinant techniques. Also disclosed are methods for utilizing such polypeptides for sterilizing fish.
1. An isolated polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 8.
2. A fusion protein comprising said isolated polypeptide of claim 1 , and a heterologous polypeptide.
3. The isolated polypeptide of claim 1 , produced by a host cell.
4. A composition comprising said isolated polypeptide of claim 1 and a pharmaceutically acceptable carrier.
5. The isolated polypeptide of claim 1 , produced by a method comprising the steps of:
(a) culturing a cell comprising the nucleic acid of SEQ ID NO: 7 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.
6. An isolated polypeptide produced by a method comprising the steps of:
(a) synthesizing said polypeptide of claim 1 using peptide synthesis; and
(b) recovering said polypeptide.
7. A method of sterilizing fish comprising administering to said fish an effective amount of a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 8.