Nell peptide expression systems and bone formation activity of nell peptide
View Patent ↗The invention generally relates to a bone growth factor, and more particularly to compositions including NELL1, articles of manufacture including NELL1 and methods of using NELL1 to induce bone formation. This invention also provides methods for the expression and purification of NELL1 and NELL2 peptides.
1. A method of expressing a functional peptide in a mammalian cell, said method comprising:
providing a nucleic acid construct including at least a nucleic acid encoding at least a NELL 1 peptide in frame with a nucleic acid encoding a secretory signal peptide;
transfecting a mammalian cell with said nucleic acid construct;
culturing said mammalian cell under conditions that permit expression of the NELL 1 peptide;
optionally collecting NELL 1 peptide secreted from the cell line;
optionally substantially purifying the NELL 1 peptide; and
optionally testing the activity of the NELL 1 peptide to induce bone formation,
wherein the secretory signal peptide is selected from the group consisting of a melittin signal sequence, a drosphila immunoglobulin-binding protein signal sequence, an equine interferon-gamma (elFN-gamma) signal peptide, a snake phospholipase A2 inhibitor signal peptide, a human lysozyme signal peptide, and a chicken lyzozyme signal peptide.
2. The method of claim 1 , wherein said mammalian cell is a COS7 cell.
3. The method of claim 1 , wherein the nucleic acid encoding NELL 1 is selected from the group comprising: SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5.
4. The method of claim 1 , wherein the NELL 1 peptide is selected from the group comprising: SEQ ID NO:2, SEQ ID NO:4, or SEQ ID NO:6.
5. A nucleic acid construct for expressing a NELL 1 peptide in a mammalian cell, said nucleic acid construct comprising at least a nucleic acid encoding at least a NELL 1 peptide in frame with a nucleic acid encoding a secretory signal peptide,
wherein the secretory signal peptide is selected from the group consisting of a melittin signal sequence, a drosphila immunoglobulin-binding protein signal sequence, an equine interferon-gamma (elFN-gamma) signal peptide. a snake phospholipase A2 inhibitor signal peptide, a human lysozyme signal peptide, and a chicken lyzozyme signal peptide.
6. The nucleic acid construct of claim 5 , wherein the nucleic acid encoding NELL 1 is selected from the group comprising: SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5.
7. The nucleic acid construct of claim 5 , wherein the NELL 1 peptide is selected from the group comprising: SEQ ID NO:2, SEQ ID NO:4, or SEQ ID NO:6.
8. A cell line for expressing a functional NELL 1 peptide, said cell line including a nucleic acid construct comprising at least a nucleic acid encoding at least a NELL 1 peptide in frame with a nucleic acid encoding a secretory signal peptide,
wherein the secretory signal peptide is selected from the group consisting of a melittin signal sequence, a drosphila immunoglobulin-binding protein signal sequence, an equine interferon-gamma (elFN-gamma) signal peptide, a snake phospholipase A2 inhibitor signal peptide, a human lysozyme signal peptide, and a chicken lyzozyme signal peptide.
9. The cell of claim 8 , wherein said cell is a mammalian cell.
10. The cell of claim 9 , wherein said cell is a COS7 cell.
11. The cell of claim 8 , wherein the secretory signal peptide is a NELL peptide signal sequence.
12. The cell of claim 8 , wherein the nucleic acid encoding NELL 1 peptide is selected from the group comprising: SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5.
13. The cell of claim 8 , wherein the NELL 1 peptide is selected from the group comprising: SEQ ID NO:2, SEQ ID NO:4, or SEQ ID NO:6.