Methods of identifying compounds useful for modulating intraflagellar transport
View Patent ↗The invention relates to various intraflagellar transport (IFT) polypeptides and the nucleic acids that encode them. The new IFT particle polypeptides and nucleic acids can be used in a variety of diagnostic, screening, and therapeutic methods.
1. A method for identifying a candidate compound that modulates flagellar function, the method comprising:
providing a sample comprising at least one isolated intraflagellar transport (IFT) particle polypeptide that comprises a sequence selected from the group consisting of SEQ ID NO: 23, 24, 25, 26, 27, 28, 30, 31, 33, 34, 36, 38, 39, and 41;
contacting said sample with a test compound;
determining whether said test compound binds to said isolated IFT particle polypeptide;
selecting a test compound that binds to said isolated IFT particle polypeptide; and
determining whether said selected test compound modulates flagellar function, thereby identifying a candidate compound that modulates flagellar function.
2. The method of claim 1 , wherein determining whether said selected test compound modulates flagellar function comprises a step of providing a sample comprising cells having flagella, and contacting said sample with said selected test compound.
3. The method of claim 2 , wherein determining whether said selected test compound modulates flagellar function comprises evaluating the size of flagella in the cells.
4. The method of claim 2 , wherein determining whether said selected test compound modulates flagellar function comprises evaluating beating of the flagella in the cells.
5. The method of claim 2 , wherein determining whether said selected test compound modulates flagellar function comprises evaluating cell motility in the sample.
6. A method for identifying a candidate compound that modulates ciliar function, the method comprising:
providing a sample comprising at least one isolated intraflagellar transport (IFT) particle polypeptide that comprises a sequence selected from the group consisting of SEQ ID NO:23, 24, 25, 26, 27, 28, 30, 31, 33, 34, 36, 38, 39, and 41;
contacting said sample with a test compound;
determining whether said test compound binds to said isolated IFT particle polypeptide;
selecting a test compound that binds to said isolated IFT particle polypeptide, and
determining whether said selected test compound modulates ciliar function, thereby identifying a candidate compound that modulates ciliar function.
7. The method of claim 6 , wherein determining whether said selected test compound modulates ciliar function comprises providing a sample comprising cells having cilia, and contacting said sample with said selected test compound.
8. The method of claim 7 , wherein determining whether said selected test compound modulates ciliar function evaluating the size of cilia in the cells.
9. The method of claim 7 , wherein determining whether said selected test compound modulates ciliar function comprises evaluating beating of cilia in the cells.