Human cord blood derived unrestricted somatic stem cells (USSC)
A composition in human cord and placental blood which comprises unrestricted somatic stem cells is described here which can be amplified in vitro to large quantities sufficient for medical applications as regenerative medicines. Initiation and maintenance as well as ex vivo expansion protocols of such stem cells from cord blood is described. Furthermore, it is shown that from these cells employing varying differentiation induction protocols distinct lineage progenitors for hematopoiesis and endothel, as well as mesenchymal progenitors for muscle bone, cartilage and fat as well as neural progenitors can be cultured and expanded for use in regenerative medicine.
1. A method for treating a cardiac muscle disease in a human patient in need of treatment for cardiac muscle disease, said method comprising administering to said patient isolated human unrestricted somatic stem cells (USSCs) from umbilical cord blood, wherein said USSCs are negative for the CD14 and CD45 antigens and positive for the CD13, CD29, CD44 and CD49e antigens, and wherein said USSCs express fibulin-2 and lack expression of hyaluronan synthase and fibromodulin, whereby said USSCs regenerate cardiac muscle cells in the heart of said patient and thereby treating said disease.